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A5JTQ2

- XYL1_MEDSV

UniProt

A5JTQ2 - XYL1_MEDSV

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Protein

Beta-xylosidase/alpha-L-arabinofuranosidase 1

Gene

Xyl1

Organism
Medicago sativa subsp. varia (Alfalfa) (Medicago varia)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

A bifunctional beta-xylosidase/alpha-L-arabinosidase, exo-enzyme that acts synergistically with endohydrolases. Releases xylose and arabinose from cell walls. Does not cleave xylan from oat spelts although xylan from oat spelts was degraded to xylose when this enzyme was used in combination with xylanase. Also releases xylose and arabinose from aryl glycosides, xylo-oligosaccharides, arabinan from sugar beet and arabino-oligosaccharides, arabinan from sugar beet and arabinoxylan from wheat.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.1 Publication
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=0.59 mM for pNP-beta-D-xlyoside (at 37 degrees Celsius)1 Publication
  2. KM=0.94 mM for pNP-alpha-L-arabinofuranoside (at 37 degrees Celsius)1 Publication
  3. KM=1.20 mM for pNP-alpha-L-arabinopyranoside (at 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei303 – 3031By similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC
  2. xylan 1,4-beta-xylosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. xylan catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-xylosidase/alpha-L-arabinofuranosidase 1Imported1 Publication
Alternative name(s):
Xylan 1,4-beta-xylosidase/Alpha-L-arabinofuranosidase 1
Short name:
MsXyl11 Publication
Including the following 2 domains:
Alternative name(s):
1,4-beta-D-xylan xylohydrolaseBy similarity
Xylan 1,4-beta-xylosidase
Alpha-L-arabinofuranosidase (EC:3.2.1.55)
Short name:
ArabinosidaseBy similarity
Gene namesi
Name:Xyl1Imported
OrganismiMedicago sativa subsp. varia (Alfalfa) (Medicago varia)
Taxonomic identifieri36902 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

Secretedextracellular spaceextracellular matrix By similarity

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 33›33Sequence AnalysisAdd
BLAST
Chaini34 – 774741Beta-xylosidase/alpha-L-arabinofuranosidase 1Sequence AnalysisPRO_0000392640Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi437 – 4371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi530 – 5301N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Proteolytically cleaved in roots to form a 65 kDa protein.1 Publication

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA5JTQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycoside hydrolase 3 family.Sequence Analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5JTQ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
ANTKNREPKV SSVFLCFSIF YVTVLLNCNH VYGQTSTVFA CDVAKNTNVS
60 70 80 90 100
SYGFCDNSLS VEDRVSDLVK RLTLQEKIGN LGNSAVEVSR LGIPKYEWWS
110 120 130 140 150
EALHGVSNIG PGTHFSSLVP GATNFPMPIL TAASFNTSLF QAIGSVVSNE
160 170 180 190 200
ARAMYNVGLA GLTYWSPNIN IFRDPRWGRG QETPGEDPLL SSKYAAGYVK
210 220 230 240 250
GLQQTDDGDS DKLKVAACCK HYTAYDVDNW KGVQRYTFDA VVSQQDLDDT
260 270 280 290 300
FQPPFKSCVI DGNVASVMCS YNKVNGKPTC ADPDLLKGVI RGKWKLNGYI
310 320 330 340 350
VSDCDSVEVL YKDQHYTKTP EEAAAKTILS GLDLDCGSYL GQYTGGAVKQ
360 370 380 390 400
GLVDEASITN AVSNNFATLM RLGFFDGDPS KQPYGNLGPK DVCTPENQEL
410 420 430 440 450
AREAARQGIV LLKNSPRSLP LSSKAIKSLA VIGPNANATR VMIGNYEGIP
460 470 480 490 500
CKYTSPLQGL TAFVPTSYAP GCPDVQCANA QIDDAAKIAA SADATIIVVG
510 520 530 540 550
ANLAIEAESL DRVNILLPGQ QQQLVNEVAN VSKGPVILVI MSGGGMDVSF
560 570 580 590 600
AKTNDKITSI LWVGYPGEAG GAAIADVIFG SYNPSGRLPM TWYPQSYVEK
610 620 630 640 650
VPMTNMNMRA DPATGYPGRT YRFYKGETVF SFGDGMSFGT VEHKIVKAPQ
660 670 680 690 700
LVSVPLAEDH ECRSLECKSL DVADKHCQNL AFDIHLSVKN MGKMSSSHSV
710 720 730 740 750
LLFFTPPNVH NAPQKHLLGF EKVQLAGKSE GMVRFKVDVC NDLSVVDELG
760 770
NRKVPLGDHM LHVGNLKHSL SVRI
Length:774
Mass (Da):83,727
Last modified:June 26, 2007 - v1
Checksum:i6C45493BA13ABF5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF569968 mRNA. Translation: ABQ45227.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF569968 mRNA. Translation: ABQ45227.1 .

3D structure databases

ProteinModelPortali A5JTQ2.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProi IPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR30620. PTHR30620. 1 hit.
Pfami PF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view ]
PRINTSi PR00133. GLHYDRLASE3.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a bifunctional beta-xylosidase/alpha-L-arabinosidase from alfalfa roots: heterologous expression in Medicago truncatula and substrate specificity of the purified enzyme."
    Xiong J.S., Balland-Vanney M., Xie Z.P., Schultze M., Kondorosi A., Kondorosi E., Staehelin C.
    J. Exp. Bot. 58:2799-2810(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PROTEOLYTIC PROCESSING.
    Strain: cv. A21 Publication.
    Tissue: Root noduleImported.

Entry informationi

Entry nameiXYL1_MEDSV
AccessioniPrimary (citable) accession number: A5JTQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: June 26, 2007
Last modified: October 29, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3