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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycoplasma agalactiae (strain PG2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36S-adenosyl-L-methionineUniRule annotation1
Binding sitei61S-adenosyl-L-methionineUniRule annotation1
Binding sitei88S-adenosyl-L-methionineUniRule annotation1
Active sitei109By similarity1
Binding sitei109S-adenosyl-L-methionineUniRule annotation1
Binding sitei113SubstrateUniRule annotation1
Binding sitei145SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAGA347257:GC07-710-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MAG6780
OrganismiMycoplasma agalactiae (strain PG2)
Taxonomic identifieri347257 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000007065 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001363561 – 205tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST205

Structurei

3D structure databases

ProteinModelPortaliA5IZB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 120Interaction with RNAUniRule annotation6
Regioni183 – 186Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IZB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRYDKNAE GKLEMFNYLI KQGDSKILLD DKTVLEIGMG KGEMIVELAK
60 70 80 90 100
AHPELNFIGL EKYPTVAAKC IKKANEYNLS NFKILIDDAF KVDEIFEGQC
110 120 130 140 150
KVIWLTFSDP WPKARHEKRR LTHPLFLEKY RKILDKDGIL KFKSDNDGLY
160 170 180 190 200
EFSLSSLQNS NWRIIDHGID LHNSKYNENN YQTGYERKWS ARGKKINYIF

AQKGE
Length:205
Mass (Da):24,047
Last modified:June 26, 2007 - v1
Checksum:iEC201514A04AA1BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU179680 Genomic DNA. Translation: CAL59378.1.
RefSeqiWP_011949831.1. NC_009497.1.

Genome annotation databases

EnsemblBacteriaiCAL59378; CAL59378; MAG6780.
KEGGimaa:MAG6780.
PATRICi32272894. VBIMycAga119067_0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU179680 Genomic DNA. Translation: CAL59378.1.
RefSeqiWP_011949831.1. NC_009497.1.

3D structure databases

ProteinModelPortaliA5IZB9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL59378; CAL59378; MAG6780.
KEGGimaa:MAG6780.
PATRICi32272894. VBIMycAga119067_0722.

Phylogenomic databases

HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciMAGA347257:GC07-710-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_MYCAP
AccessioniPrimary (citable) accession number: A5IZB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.