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A5IY09

- SYI_MYCAP

UniProt

A5IY09 - SYI_MYCAP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma agalactiae (strain PG2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi862 – 8621ZincUniRule annotation
    Metal bindingi865 – 8651ZincUniRule annotation
    Metal bindingi879 – 8791ZincUniRule annotation
    Metal bindingi882 – 8821ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMAGA347257:GC07-237-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MAG2200
    OrganismiMycoplasma agalactiae (strain PG2)
    Taxonomic identifieri347257 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000007065: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 892892Isoleucine--tRNA ligasePRO_1000189181Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi347257.MAG_2200.

    Structurei

    3D structure databases

    ProteinModelPortaliA5IY09.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5IY09-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEIDYKETL NMPKTDFEMR ANLTTKEPLF REKWEKDNLY ARVLEKNKNN    50
    TPFVLHDGPP YANGSIHIGH ALNKILKDII VRYKSMCGFY SPYVPGWDTH 100
    GLPIELKMLT DAKINYKVIS PIELRKRASE YADIQIENQI SQFKSLQLLT 150
    DFKKIYKTKE PKFEAAQLKL FKKMVLDGLV YKGLKPVYWS PSSQTALAEA 200
    EVEYHDVDSP SIYVALEIID QNGSQKVKNG DKLVIWTTTP WTLLANAAVA 250
    IGENFVYCRV EHNNQGYIVA KELANKFIEI SKLDNAQISV DFNANELIGI 300
    KYKSVLNDLV CPVVIGHHVS LESGTGLVHI APLFGEDDFQ IGTANSLEMI 350
    MHVEDDGVLN DAAGKYKGIF YEKANAQIFD KLTSKSALLA KGTIRHSYPH 400
    DWRTHKPILF RGTPQWFVSI DKIRQQLLNE LESINTYPEW AKKRLVNMIS 450
    ERKDWTISRQ RTWGVPIIIF YDKDGKPVIN SAIFDYVIDL VEKYGTDIWW 500
    EKEADELLPE EFRSNGYTKE MDIMDVWFDS GTTSLAVEID EKLKPPYDLY 550
    LEGSDQYRGW FNSSLINSVA YTHKSPYKQI VSHGFVLDSK GEKMSKSKGN 600
    VVDPLKVIKK YGADILRLWV ANAEYTNDVN ISDEIINQNS EIYRKIRNTI 650
    KFLLGNLNGY EYDESVKRTG VHQYIYNELE SIKEKVYKAY DEYNFSSVIK 700
    TINKYVVELS SFYLNITKDI LYVHEFDSNE RMMTLANFYD ITNFLIKAIA 750
    PIIPTTAEDA YMHFYKKNKL ESIHLENFDI VKPYDSNVLK EWEEFFSLKD 800
    EVNLLLENAI KSGLIKRTNE AKVTIKNPSE VIKGYDLKQL LMVGAIEFGN 850
    VSKVESFMSE KCNRCWNHFS PAQIKDNLCP LCYKIVQKVN GK 892
    Length:892
    Mass (Da):102,793
    Last modified:June 26, 2007 - v1
    Checksum:i84FAA196980A3941
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU179680 Genomic DNA. Translation: CAL58918.1.
    RefSeqiWP_011949399.1. NC_009497.1.
    YP_001256362.1. NC_009497.1.

    Genome annotation databases

    EnsemblBacteriaiCAL58918; CAL58918; MAG2200.
    GeneIDi5183418.
    KEGGimaa:MAG_2200.
    PATRICi32271886. VBIMycAga119067_0236.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU179680 Genomic DNA. Translation: CAL58918.1 .
    RefSeqi WP_011949399.1. NC_009497.1.
    YP_001256362.1. NC_009497.1.

    3D structure databases

    ProteinModelPortali A5IY09.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 347257.MAG_2200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL58918 ; CAL58918 ; MAG2200 .
    GeneIDi 5183418.
    KEGGi maa:MAG_2200.
    PATRICi 32271886. VBIMycAga119067_0236.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MAGA347257:GC07-237-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PG2.

    Entry informationi

    Entry nameiSYI_MYCAP
    AccessioniPrimary (citable) accession number: A5IY09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3