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A5IY09

- SYI_MYCAP

UniProt

A5IY09 - SYI_MYCAP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma agalactiae (strain PG2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi862 – 8621ZincUniRule annotation
Metal bindingi865 – 8651ZincUniRule annotation
Metal bindingi879 – 8791ZincUniRule annotation
Metal bindingi882 – 8821ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMAGA347257:GC07-237-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MAG2200
OrganismiMycoplasma agalactiae (strain PG2)
Taxonomic identifieri347257 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000007065: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Isoleucine--tRNA ligasePRO_1000189181Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi347257.MAG_2200.

Structurei

3D structure databases

ProteinModelPortaliA5IY09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IY09-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEIDYKETL NMPKTDFEMR ANLTTKEPLF REKWEKDNLY ARVLEKNKNN
60 70 80 90 100
TPFVLHDGPP YANGSIHIGH ALNKILKDII VRYKSMCGFY SPYVPGWDTH
110 120 130 140 150
GLPIELKMLT DAKINYKVIS PIELRKRASE YADIQIENQI SQFKSLQLLT
160 170 180 190 200
DFKKIYKTKE PKFEAAQLKL FKKMVLDGLV YKGLKPVYWS PSSQTALAEA
210 220 230 240 250
EVEYHDVDSP SIYVALEIID QNGSQKVKNG DKLVIWTTTP WTLLANAAVA
260 270 280 290 300
IGENFVYCRV EHNNQGYIVA KELANKFIEI SKLDNAQISV DFNANELIGI
310 320 330 340 350
KYKSVLNDLV CPVVIGHHVS LESGTGLVHI APLFGEDDFQ IGTANSLEMI
360 370 380 390 400
MHVEDDGVLN DAAGKYKGIF YEKANAQIFD KLTSKSALLA KGTIRHSYPH
410 420 430 440 450
DWRTHKPILF RGTPQWFVSI DKIRQQLLNE LESINTYPEW AKKRLVNMIS
460 470 480 490 500
ERKDWTISRQ RTWGVPIIIF YDKDGKPVIN SAIFDYVIDL VEKYGTDIWW
510 520 530 540 550
EKEADELLPE EFRSNGYTKE MDIMDVWFDS GTTSLAVEID EKLKPPYDLY
560 570 580 590 600
LEGSDQYRGW FNSSLINSVA YTHKSPYKQI VSHGFVLDSK GEKMSKSKGN
610 620 630 640 650
VVDPLKVIKK YGADILRLWV ANAEYTNDVN ISDEIINQNS EIYRKIRNTI
660 670 680 690 700
KFLLGNLNGY EYDESVKRTG VHQYIYNELE SIKEKVYKAY DEYNFSSVIK
710 720 730 740 750
TINKYVVELS SFYLNITKDI LYVHEFDSNE RMMTLANFYD ITNFLIKAIA
760 770 780 790 800
PIIPTTAEDA YMHFYKKNKL ESIHLENFDI VKPYDSNVLK EWEEFFSLKD
810 820 830 840 850
EVNLLLENAI KSGLIKRTNE AKVTIKNPSE VIKGYDLKQL LMVGAIEFGN
860 870 880 890
VSKVESFMSE KCNRCWNHFS PAQIKDNLCP LCYKIVQKVN GK
Length:892
Mass (Da):102,793
Last modified:June 26, 2007 - v1
Checksum:i84FAA196980A3941
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU179680 Genomic DNA. Translation: CAL58918.1.
RefSeqiWP_011949399.1. NC_009497.1.
YP_001256362.1. NC_009497.1.

Genome annotation databases

EnsemblBacteriaiCAL58918; CAL58918; MAG2200.
GeneIDi5183418.
KEGGimaa:MAG_2200.
PATRICi32271886. VBIMycAga119067_0236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU179680 Genomic DNA. Translation: CAL58918.1 .
RefSeqi WP_011949399.1. NC_009497.1.
YP_001256362.1. NC_009497.1.

3D structure databases

ProteinModelPortali A5IY09.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 347257.MAG_2200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL58918 ; CAL58918 ; MAG2200 .
GeneIDi 5183418.
KEGGi maa:MAG_2200.
PATRICi 32271886. VBIMycAga119067_0236.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MAGA347257:GC07-237-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PG2.

Entry informationi

Entry nameiSYI_MYCAP
AccessioniPrimary (citable) accession number: A5IY09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 26, 2007
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3