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A5IY09

- SYI_MYCAP

UniProt

A5IY09 - SYI_MYCAP

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Protein

Isoleucine--tRNA ligase

Gene
ileS, MAG2200
Organism
Mycoplasma agalactiae (strain PG2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi862 – 8621Zinc By similarity
Metal bindingi865 – 8651Zinc By similarity
Metal bindingi879 – 8791Zinc By similarity
Metal bindingi882 – 8821Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMAGA347257:GC07-237-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MAG2200
OrganismiMycoplasma agalactiae (strain PG2)
Taxonomic identifieri347257 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000007065: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Isoleucine--tRNA ligaseUniRule annotationPRO_1000189181Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi347257.MAG_2200.

Structurei

3D structure databases

ProteinModelPortaliA5IY09.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotationAdd
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IY09-1 [UniParc]FASTAAdd to Basket

« Hide

MSEIDYKETL NMPKTDFEMR ANLTTKEPLF REKWEKDNLY ARVLEKNKNN    50
TPFVLHDGPP YANGSIHIGH ALNKILKDII VRYKSMCGFY SPYVPGWDTH 100
GLPIELKMLT DAKINYKVIS PIELRKRASE YADIQIENQI SQFKSLQLLT 150
DFKKIYKTKE PKFEAAQLKL FKKMVLDGLV YKGLKPVYWS PSSQTALAEA 200
EVEYHDVDSP SIYVALEIID QNGSQKVKNG DKLVIWTTTP WTLLANAAVA 250
IGENFVYCRV EHNNQGYIVA KELANKFIEI SKLDNAQISV DFNANELIGI 300
KYKSVLNDLV CPVVIGHHVS LESGTGLVHI APLFGEDDFQ IGTANSLEMI 350
MHVEDDGVLN DAAGKYKGIF YEKANAQIFD KLTSKSALLA KGTIRHSYPH 400
DWRTHKPILF RGTPQWFVSI DKIRQQLLNE LESINTYPEW AKKRLVNMIS 450
ERKDWTISRQ RTWGVPIIIF YDKDGKPVIN SAIFDYVIDL VEKYGTDIWW 500
EKEADELLPE EFRSNGYTKE MDIMDVWFDS GTTSLAVEID EKLKPPYDLY 550
LEGSDQYRGW FNSSLINSVA YTHKSPYKQI VSHGFVLDSK GEKMSKSKGN 600
VVDPLKVIKK YGADILRLWV ANAEYTNDVN ISDEIINQNS EIYRKIRNTI 650
KFLLGNLNGY EYDESVKRTG VHQYIYNELE SIKEKVYKAY DEYNFSSVIK 700
TINKYVVELS SFYLNITKDI LYVHEFDSNE RMMTLANFYD ITNFLIKAIA 750
PIIPTTAEDA YMHFYKKNKL ESIHLENFDI VKPYDSNVLK EWEEFFSLKD 800
EVNLLLENAI KSGLIKRTNE AKVTIKNPSE VIKGYDLKQL LMVGAIEFGN 850
VSKVESFMSE KCNRCWNHFS PAQIKDNLCP LCYKIVQKVN GK 892
Length:892
Mass (Da):102,793
Last modified:June 26, 2007 - v1
Checksum:i84FAA196980A3941
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU179680 Genomic DNA. Translation: CAL58918.1.
RefSeqiWP_011949399.1. NC_009497.1.
YP_001256362.1. NC_009497.1.

Genome annotation databases

EnsemblBacteriaiCAL58918; CAL58918; MAG2200.
GeneIDi5183418.
KEGGimaa:MAG_2200.
PATRICi32271886. VBIMycAga119067_0236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU179680 Genomic DNA. Translation: CAL58918.1 .
RefSeqi WP_011949399.1. NC_009497.1.
YP_001256362.1. NC_009497.1.

3D structure databases

ProteinModelPortali A5IY09.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 347257.MAG_2200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL58918 ; CAL58918 ; MAG2200 .
GeneIDi 5183418.
KEGGi maa:MAG_2200.
PATRICi 32271886. VBIMycAga119067_0236.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MAGA347257:GC07-237-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PG2.

Entry informationi

Entry nameiSYI_MYCAP
AccessioniPrimary (citable) accession number: A5IY09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 26, 2007
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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