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Protein

L-lactate dehydrogenase 2

Gene

ldh2

Organism
Staphylococcus aureus (strain JH9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Contributes to S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells, despite playing a secondary role in this resistance mechanism.By similarity

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91SubstrateBy similarity1
Binding sitei123NAD or substrateBy similarity1
Binding sitei154SubstrateBy similarity1
Active sitei178Proton acceptorBy similarity1
Binding sitei231SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 42NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 2 (EC:1.1.1.27)
Short name:
L-LDH 2
Gene namesi
Name:ldh2
Ordered Locus Names:SaurJH9_2624
OrganismiStaphylococcus aureus (strain JH9)
Taxonomic identifieri359786 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003438371 – 319L-lactate dehydrogenase 2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA5IW27.
SMRiA5IW27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiDQWKNIH.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

A5IW27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTFGKKVVL IGDGSVGSSY AFAMVTQGVA DEFVIIDIAK DKVKADVQDL
60 70 80 90 100
NHGTVHSPSP VDVKAGEYED CKDADLVVIT AGAPQKPGET RLQLVEKNTK
110 120 130 140 150
IMKSIVKSVM DSGFDGYFLI AANPVDILTR FVKEYTGLPA ERVIGSGTVL
160 170 180 190 200
DSARLQYLIS QELGVAPSSV DASIIGEHGD TELAVWSQAN VAGISVYDTL
210 220 230 240 250
KEQTGSEAKA EEIYVNTRDA AYEIIQAKGS TYYGIALALM RISKAILNNE
260 270 280 290 300
NNVLNVSIQL DGQYGGHKGV YLGVPTLVNQ HGAVKIYEMP LSAEEQALFD
310
KSVKILEDTF DSIKYLLED
Length:319
Mass (Da):34,432
Last modified:June 26, 2007 - v1
Checksum:i7779343BCA801CED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ50400.1.
RefSeqiWP_000846636.1. NC_009487.1.

Genome annotation databases

EnsemblBacteriaiABQ50400; ABQ50400; SaurJH9_2624.
KEGGisaj:SaurJH9_2624.

Similar proteinsi

Entry informationi

Entry nameiLDH2_STAA9
AccessioniPrimary (citable) accession number: A5IW27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: June 26, 2007
Last modified: October 25, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families