A5IVH2 (NREC_STAA9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxygen regulatory protein NreC

Alternative name(s):
Nitrogen regulation protein C

Gene names

Name:	nreC
Ordered Locus Names:	SaurJH9_2415

Organism

Staphylococcus aureus (strain JH9) [Complete proteome] [HAMAP]

Taxonomic identifier

359786 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. Phosphorylated NreC binds to a GC-rich palindromic sequence at the promoters of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT, and activates their expression By similarity.
Subcellular location	Cytoplasm Potential.
Post-translational modification	Phosphorylated by NreB By similarity.
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Oxygen regulatory protein NreC		PRO_0000349345
Regions
	Domain	2 – 119	118	Response regulatory
	Domain	148 – 213	66	HTH luxR-type
	DNA binding	172 – 191	20	H-T-H motif By similarity
Amino acid modifications
	Modified residue	53	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5IVH2 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: DDF2969C5B277320	FASTA	217	24,368
	10 20 30 40 50 60 MKIVIADDHA VVRTGFSMIL NYQNDMEVVA TAADGVEAYQ KVMEYKPDVL LMDLSMPPGE 70 80 90 100 110 120 SGLIATSKIA DSFPETKILI LTMFDDEEYL FHVLRNGAKG YILKNAPDEQ LLLAIRTVYK 130 140 150 160 170 180 GETYVDMKLT TSLVNEFVSN SNQDTANTTD PFKILSKREL EILPLIAKGY GNKEIAEKLF 190 200 210 VSVKTVEAHK THIMTKLGLK SKPELVEYAL KKKLLEF

References

[1]

"Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH9." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Tomasz A., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JH9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000703 Genomic DNA. Translation: ABQ50195.1.
RefSeq	YP_001247771.1. NC_009487.1.
3D structure databases
ProteinModelPortal	A5IVH2.
SMR	A5IVH2. Positions 1-215.
ModBase	Search...
Protein-protein interaction databases
STRING	359786.SaurJH9_2415.
Proteomic databases
PRIDE	A5IVH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ50195; ABQ50195; SaurJH9_2415.
GeneID	5168495.
KEGG	saj:SaurJH9_2415.
PATRIC	19542831. VBIStaAur42398_2554.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2197.
HOGENOM	HOG000034813.
KO	K07696.
OMA	FKEADWI.
ProtClustDB	CLSK885949.
Enzyme and pathway databases
BioCyc	SAUR359786:GJEM-2498-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR016032. Sig_transdc_resp-reg_C-effctor. IPR001789. Sig_transdc_resp-reg_receiver. IPR000792. Tscrpt_reg_LuxR_C. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00196. GerE. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00038. HTHLUXR.
SMART	SM00421. HTH_LUXR. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF46894. Bipartite_resp_reg_C-effector. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS00622. HTH_LUXR_1. 1 hit. PS50043. HTH_LUXR_2. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NREC_STAA9

Accession

Primary (citable) accession number: A5IVH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program