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Protein

Shikimate kinase

Gene

aroK

Organism
Staphylococcus aureus (strain JH9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.UniRule annotation

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19MagnesiumUniRule annotation1
Binding sitei37SubstrateUniRule annotation1
Binding sitei61SubstrateUniRule annotation1
Binding sitei82Substrate; via amide nitrogenUniRule annotation1
Binding sitei120ATPUniRule annotation1
Binding sitei138SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 20ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
Gene namesi
Name:aroKUniRule annotation
Ordered Locus Names:SaurJH9_1596
OrganismiStaphylococcus aureus (strain JH9)
Taxonomic identifieri359786 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000759611 – 174Shikimate kinaseAdd BLAST174

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5IT66.
SMRiA5IT66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000032569.
KOiK00891.
OMAiTTIGSHL.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IT66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHDKSPIIL IGFMGTGKST IGKYVADEQN LSFIDIDSYI EEKYKLTIPE
60 70 80 90 100
IFSKHGEQYF RNLEFTCLQE CINTADIIAT GGGIIESEEA FNFLKNQKNI
110 120 130 140 150
IWLDCNIDII YSRINDDPHR PNANNKTIKQ LNDLYCSRIL RYNEIAFKKF
160 170
DSHLLSISEI YYELLNLIKA SDQY
Length:174
Mass (Da):20,205
Last modified:June 26, 2007 - v1
Checksum:i0A93299B320B0968
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ49389.1.
RefSeqiWP_001015120.1. NC_009487.1.

Genome annotation databases

EnsemblBacteriaiABQ49389; ABQ49389; SaurJH9_1596.
KEGGisaj:SaurJH9_1596.
PATRICi19540994. VBIStaAur42398_1687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ49389.1.
RefSeqiWP_001015120.1. NC_009487.1.

3D structure databases

ProteinModelPortaliA5IT66.
SMRiA5IT66.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ49389; ABQ49389; SaurJH9_1596.
KEGGisaj:SaurJH9_1596.
PATRICi19540994. VBIStaAur42398_1687.

Phylogenomic databases

HOGENOMiHOG000032569.
KOiK00891.
OMAiTTIGSHL.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROK_STAA9
AccessioniPrimary (citable) accession number: A5IT66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.