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Protein

Ribonuclease Z

Gene

rnz

Organism
Staphylococcus aureus (strain JH9)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Zinc 1; catalyticUniRule annotation1
Metal bindingi65Zinc 1; catalyticUniRule annotation1
Active sitei67Proton acceptorUniRule annotation1
Metal bindingi67Zinc 2; catalyticUniRule annotation1
Metal bindingi68Zinc 2; catalyticUniRule annotation1
Metal bindingi141Zinc 1; catalyticUniRule annotation1
Metal bindingi211Zinc 1; catalyticUniRule annotation1
Metal bindingi211Zinc 2; catalyticUniRule annotation1
Metal bindingi269Zinc 2; catalyticUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Ordered Locus Names:SaurJH9_1561
OrganismiStaphylococcus aureus (strain JH9)
Taxonomic identifieri359786 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000883431 – 306Ribonuclease ZAdd BLAST306

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5IT32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000272419.
KOiK00784.
OMAiIFFTHYH.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

A5IT32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVTFFGTSA GLPTKERNTQ AIALNLEPYS NSIWLFDVGE GTQHQILHHA
60 70 80 90 100
IKLGKVTHIF ITHMHGDHIF GLPGLLSSRS FQGGEQKPLT LVGPKGIKAY
110 120 130 140 150
VEMSMNLSES HLNYPITYIE IDDHLTYHHD GFTVEAHLLN HGIPSYGYRV
160 170 180 190 200
MAPETTGTIN VEALKNIGLE PGPKYQEVKS HDTFEHNGQV YQSKDFRGES
210 220 230 240 250
KQGPVVAIFG DTKPCSNERV ISRDADVMVH EATYIDGEKH LANNYHHSHI
260 270 280 290 300
EDVFALIKEA NVKRTLITHL SNRYNTEDIN EIYQILIQNE DTPNFNFVKD

FDSFKV
Length:306
Mass (Da):34,583
Last modified:June 26, 2007 - v1
Checksum:i5B0F75A23CD0F15F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ49355.1.
RefSeqiWP_000454063.1. NC_009487.1.

Genome annotation databases

EnsemblBacteriaiABQ49355; ABQ49355; SaurJH9_1561.
KEGGisaj:SaurJH9_1561.
PATRICi19540922. VBIStaAur42398_1651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ49355.1.
RefSeqiWP_000454063.1. NC_009487.1.

3D structure databases

ProteinModelPortaliA5IT32.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ49355; ABQ49355; SaurJH9_1561.
KEGGisaj:SaurJH9_1561.
PATRICi19540922. VBIStaAur42398_1651.

Phylogenomic databases

HOGENOMiHOG000272419.
KOiK00784.
OMAiIFFTHYH.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNZ_STAA9
AccessioniPrimary (citable) accession number: A5IT32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 26, 2007
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.