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Protein

Glycerol kinase

Gene

glpK

Organism
Staphylococcus aureus (strain JH9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121SubstrateUniRule annotation
Binding sitei16 – 161ATPUniRule annotation
Binding sitei134 – 1341SubstrateUniRule annotation
Binding sitei266 – 2661ATPUniRule annotation
Binding sitei309 – 3091ATP; via carbonyl oxygenUniRule annotation
Binding sitei313 – 3131ATP; via amide nitrogenUniRule annotation
Binding sitei328 – 3281ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 143ATPUniRule annotation
Nucleotide bindingi410 – 4145ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR359786:GJEM-1386-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:SaurJH9_1359
OrganismiStaphylococcus aureus (strain JH9)
Taxonomic identifieri359786 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Glycerol kinasePRO_1000077433Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301Phosphohistidine; by HPrUniRule annotation

Post-translational modificationi

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA5ISI2.

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5ISI2.
SMRiA5ISI2. Positions 1-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingUniRule annotation
Regioni244 – 2452Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000222134.
KOiK00864.
OMAiPMEIFAT.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5ISI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYILSIDQ GTTSSRAILF NQKGEIAGVA QREFKQYFPQ SGWVEHDANE
60 70 80 90 100
IWTSVLAVMT EVINENDVRA DQIAGIGITN QRETTVVWDK HTGRPIYHAI
110 120 130 140 150
VWQSRQTQSI CSELKQQGYE QTFRDKTGLL LDPYFAGTKV KWILDNVEGA
160 170 180 190 200
REKAENGDLL FGTIDTWLVW KLSGKAAHIT DYSNASRTLM FNIHDLEWDD
210 220 230 240 250
ELLELLTVPK NMLPEVKPSS EIYGKTIDYH FYGQEVPIAG VAGDQQAALF
260 270 280 290 300
GQACFECGDV KNTYGTGGFM LMNTGDKAVK SESGLLTTIA YGIDGKVNYA
310 320 330 340 350
LEGSIFVSGS AIQWLRDGLR MINSAPQSES YATRVDSTEG VYVVPAFVGL
360 370 380 390 400
GTPYWDSEAR GAIFGLTRGT EKEHFIRATL ESLCYQTRDV MEAMSKDSGI
410 420 430 440 450
DVQSLRVDGG AVKNNFIMQF QADIVNTSVE RPEIQETTAL GAAFLAGLAV
460 470 480 490
GFWESKDDIA KNWKLEEKFD PKMDEGEREK LYRGWKKAVE ATQVFKTE
Length:498
Mass (Da):55,613
Last modified:June 26, 2007 - v1
Checksum:iC20BCE90B3CA83F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ49155.1.
RefSeqiWP_000417372.1. NC_009487.1.

Genome annotation databases

EnsemblBacteriaiABQ49155; ABQ49155; SaurJH9_1359.
KEGGisaj:SaurJH9_1359.
PATRICi19540502. VBIStaAur42398_1441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000703 Genomic DNA. Translation: ABQ49155.1.
RefSeqiWP_000417372.1. NC_009487.1.

3D structure databases

ProteinModelPortaliA5ISI2.
SMRiA5ISI2. Positions 1-498.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA5ISI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ49155; ABQ49155; SaurJH9_1359.
KEGGisaj:SaurJH9_1359.
PATRICi19540502. VBIStaAur42398_1441.

Phylogenomic databases

HOGENOMiHOG000222134.
KOiK00864.
OMAiPMEIFAT.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciSAUR359786:GJEM-1386-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK_STAA9
AccessioniPrimary (citable) accession number: A5ISI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 26, 2007
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.