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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881MagnesiumUniRule annotation
Metal bindingi494 – 4941MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciTPET390874:GHJI-1484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Tpet_1438
OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Taxonomic identifieri390874 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000006558: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Polyribonucleotide nucleotidyltransferasePRO_0000329914Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi390874.Tpet_1438.

Structurei

3D structure databases

ProteinModelPortaliA5IMM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61561KHUniRule annotationAdd
BLAST
Domaini625 – 69268S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF13014. KH_3. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IMM8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEWRRNILG RELVVQYGKV AKQSSGSALV RFGDTVVLAT ANISDKAVEG
60 70 80 90 100
IDFVPLTVEF QERFYAAGKI PGGFIKREGK PSESAILSAR LIDRPIRPLF
110 120 130 140 150
PKKLRNEVQV IVTVLSVDPN VPPDVVGIFA ASLALNVSKI PFEGIVAGIR
160 170 180 190 200
VGYRDGQFIA LPSEEDIEKG LMDITVAGTK DAVTMVEGEA KEVAEEDMVK
210 220 230 240 250
ALRFAHSVIK ELVDFQEEIL SEFNVEKIPV VEPTPPEGLV EAFKDLLNKE
260 270 280 290 300
ELERRILVKV KKEREVALKE YEEQLLNQIA EKLSVTDLEG IKPFVSELYE
310 320 330 340 350
DAVKKTMRRL IVEKGIRADG RKPTEIRPIS CEVGLFPRTH GSALFTRGET
360 370 380 390 400
QSLGIVTLGA PMDVQIIDTL LEEGVKRFML HYNFPPFCTG EVKPLRGPSR
410 420 430 440 450
REIGHGHLAE RALKNMLPPE EEFPYTIRVV SEILESNGSS SMATVCSGSL
460 470 480 490 500
ALMDAGVPIK KHVAGIAMGL ILEEDAEIIL TDIIGMEDHY GDMDFKVAGT
510 520 530 540 550
RDGITAFQMD CKVSGVSDEL LMKALMQARE ARMYILDRMY ETISAPRPHL
560 570 580 590 600
SKYAPIIKVT KVDPEKVADV IGPGGRVIKK IIKDFDVKVE IDDETGLVKV
610 620 630 640 650
VGSSEENVDK AIELIREIAK EIEVGEVLEG KVTRIEPYGL FIEVRPGKIG
660 670 680 690 700
LLHQSKVGED MRQFLKKVKV GDTIKVQVIN IDDLGRLQFK RVTEGENTQH

GKTHSKRN
Length:708
Mass (Da):78,591
Last modified:June 26, 2007 - v1
Checksum:iEC60D4CE5F025BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ47451.1.
RefSeqiYP_001245027.1. NC_009486.1.

Genome annotation databases

EnsemblBacteriaiABQ47451; ABQ47451; Tpet_1438.
GeneIDi5170628.
KEGGitpt:Tpet_1438.
PATRICi23945723. VBIThePet65348_1458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ47451.1.
RefSeqiYP_001245027.1. NC_009486.1.

3D structure databases

ProteinModelPortaliA5IMM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390874.Tpet_1438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ47451; ABQ47451; Tpet_1438.
GeneIDi5170628.
KEGGitpt:Tpet_1438.
PATRICi23945723. VBIThePet65348_1458.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciTPET390874:GHJI-1484-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF13014. KH_3. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Entry informationi

Entry nameiPNP_THEP1
AccessioniPrimary (citable) accession number: A5IMM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 26, 2007
Last modified: February 4, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.