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A5IML2

- SYI_THEP1

UniProt

A5IML2 - SYI_THEP1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
    Binding sitei597 – 5971ATPUniRule annotation
    Metal bindingi887 – 8871ZincUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTPET390874:GHJI-1468-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Tpet_1422
    OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
    Taxonomic identifieri390874 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000006558: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000022138Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi390874.Tpet_1422.

    Structurei

    3D structure databases

    ProteinModelPortaliA5IML2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5IML2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKNTLNLP KTSFPMKANL VNKEKVFLEE WEKMDLYNYV LEQRKGKPLF    50
    VLHDGPPYAN GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP 100
    IEHRVSQELG EKIKEMSPAE IRKKCEEFAL RFVDIQREEF KRLGVRGDWE 150
    NPYITLKPDY EVKILDVFKT LVEEGNVYRS LKPIYWCPRC RTALAEAEIE 200
    YHDHKSPSIY VKFRSKEDPN FFIVIWTTTP WTLPANVGIA LHPDYEYSVV 250
    KVGEEKWVIA TDLLDAFSKE TGIDCSNVVE KIKGKDLEGK EFVHPIFDDK 300
    TSRVILADYV SLETGTGCVH IAPGHGEEDY IYGHVQYGLP IVSPVDEEGR 350
    FTEEAGKYKG MFIEDANEVI IEDLKKKGIL VHASSITHSY PHCWRCKGPV 400
    IFRATEQWFI SVDHNNLRQK VLEEIDKVKW IPEWGRNRIR SMVEERPDWC 450
    ISRQRVWGTP IPAVKCKECG EVTLDPKVIE HFMKIVEKEG TNAWFEKEVE 500
    ELIPEDFVCP KCGKRSFEKM LDTLDVWIDS GASFEYITTK REDHPFPLDM 550
    YLEGSDQHRG WFHSSIFLAV AKRGSAPYKE VLTHGFIKDE QGRKMSKSLG 600
    NVVDPMEVVE KYGAEILRLW LASSDYFNDI KISMRIVEQQ TEVYRKIRNT 650
    FRFLLGNLEG FDPELDRVPY EKLLTIDRWA LGRLQEIIKR ATEYYDSYEF 700
    SKVYNLVVKY CTTELSSLYL DVVKDRLYVE AKDSIYRRSA QTVMHEILIA 750
    LMKILAPIMT FTMEEVYSHL HEKDRKYKTV QAEYWPEYKE EFIDRELMED 800
    FEKLLSIRED VLKALEEKRQ QDVIGHSLDA EVVLVPKNDT IKALLEKYRD 850
    ILEELFIVSK VSLSDASGEL KGELVEVTVK HAEGEKCQRC WKYTTEISTS 900
    EDFPGVCPRC LAVLKGERK 919
    Length:919
    Mass (Da):107,044
    Last modified:June 26, 2007 - v1
    Checksum:iC80CC27CF8AA62DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000702 Genomic DNA. Translation: ABQ47435.1.
    RefSeqiWP_011943889.1. NC_009486.1.
    YP_001245011.1. NC_009486.1.

    Genome annotation databases

    EnsemblBacteriaiABQ47435; ABQ47435; Tpet_1422.
    GeneIDi5171003.
    KEGGitpt:Tpet_1422.
    PATRICi23945691. VBIThePet65348_1442.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000702 Genomic DNA. Translation: ABQ47435.1 .
    RefSeqi WP_011943889.1. NC_009486.1.
    YP_001245011.1. NC_009486.1.

    3D structure databases

    ProteinModelPortali A5IML2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390874.Tpet_1422.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ47435 ; ABQ47435 ; Tpet_1422 .
    GeneIDi 5171003.
    KEGGi tpt:Tpet_1422.
    PATRICi 23945691. VBIThePet65348_1442.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TPET390874:GHJI-1468-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

    Entry informationi

    Entry nameiSYI_THEP1
    AccessioniPrimary (citable) accession number: A5IML2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3