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A5IML2

- SYI_THEP1

UniProt

A5IML2 - SYI_THEP1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
Binding sitei597 – 5971ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTPET390874:GHJI-1468-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tpet_1422
OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Taxonomic identifieri390874 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000006558: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000022138Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi390874.Tpet_1422.

Structurei

3D structure databases

ProteinModelPortaliA5IML2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IML2-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDYKNTLNLP KTSFPMKANL VNKEKVFLEE WEKMDLYNYV LEQRKGKPLF
60 70 80 90 100
VLHDGPPYAN GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP
110 120 130 140 150
IEHRVSQELG EKIKEMSPAE IRKKCEEFAL RFVDIQREEF KRLGVRGDWE
160 170 180 190 200
NPYITLKPDY EVKILDVFKT LVEEGNVYRS LKPIYWCPRC RTALAEAEIE
210 220 230 240 250
YHDHKSPSIY VKFRSKEDPN FFIVIWTTTP WTLPANVGIA LHPDYEYSVV
260 270 280 290 300
KVGEEKWVIA TDLLDAFSKE TGIDCSNVVE KIKGKDLEGK EFVHPIFDDK
310 320 330 340 350
TSRVILADYV SLETGTGCVH IAPGHGEEDY IYGHVQYGLP IVSPVDEEGR
360 370 380 390 400
FTEEAGKYKG MFIEDANEVI IEDLKKKGIL VHASSITHSY PHCWRCKGPV
410 420 430 440 450
IFRATEQWFI SVDHNNLRQK VLEEIDKVKW IPEWGRNRIR SMVEERPDWC
460 470 480 490 500
ISRQRVWGTP IPAVKCKECG EVTLDPKVIE HFMKIVEKEG TNAWFEKEVE
510 520 530 540 550
ELIPEDFVCP KCGKRSFEKM LDTLDVWIDS GASFEYITTK REDHPFPLDM
560 570 580 590 600
YLEGSDQHRG WFHSSIFLAV AKRGSAPYKE VLTHGFIKDE QGRKMSKSLG
610 620 630 640 650
NVVDPMEVVE KYGAEILRLW LASSDYFNDI KISMRIVEQQ TEVYRKIRNT
660 670 680 690 700
FRFLLGNLEG FDPELDRVPY EKLLTIDRWA LGRLQEIIKR ATEYYDSYEF
710 720 730 740 750
SKVYNLVVKY CTTELSSLYL DVVKDRLYVE AKDSIYRRSA QTVMHEILIA
760 770 780 790 800
LMKILAPIMT FTMEEVYSHL HEKDRKYKTV QAEYWPEYKE EFIDRELMED
810 820 830 840 850
FEKLLSIRED VLKALEEKRQ QDVIGHSLDA EVVLVPKNDT IKALLEKYRD
860 870 880 890 900
ILEELFIVSK VSLSDASGEL KGELVEVTVK HAEGEKCQRC WKYTTEISTS
910
EDFPGVCPRC LAVLKGERK
Length:919
Mass (Da):107,044
Last modified:June 26, 2007 - v1
Checksum:iC80CC27CF8AA62DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ47435.1.
RefSeqiYP_001245011.1. NC_009486.1.

Genome annotation databases

EnsemblBacteriaiABQ47435; ABQ47435; Tpet_1422.
GeneIDi5171003.
KEGGitpt:Tpet_1422.
PATRICi23945691. VBIThePet65348_1442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ47435.1 .
RefSeqi YP_001245011.1. NC_009486.1.

3D structure databases

ProteinModelPortali A5IML2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 390874.Tpet_1422.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ47435 ; ABQ47435 ; Tpet_1422 .
GeneIDi 5171003.
KEGGi tpt:Tpet_1422.
PATRICi 23945691. VBIThePet65348_1442.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TPET390874:GHJI-1468-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Entry informationi

Entry nameiSYI_THEP1
AccessioniPrimary (citable) accession number: A5IML2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3