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A5IML2

- SYI_THEP1

UniProt

A5IML2 - SYI_THEP1

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Tpet_1422
Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi887 – 8871Zinc By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTPET390874:GHJI-1468-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Tpet_1422
OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Taxonomic identifieri390874 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000006558: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligaseUniRule annotationPRO_1000022138Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi390874.Tpet_1422.

Structurei

3D structure databases

ProteinModelPortaliA5IML2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IML2-1 [UniParc]FASTAAdd to Basket

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MDYKNTLNLP KTSFPMKANL VNKEKVFLEE WEKMDLYNYV LEQRKGKPLF    50
VLHDGPPYAN GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP 100
IEHRVSQELG EKIKEMSPAE IRKKCEEFAL RFVDIQREEF KRLGVRGDWE 150
NPYITLKPDY EVKILDVFKT LVEEGNVYRS LKPIYWCPRC RTALAEAEIE 200
YHDHKSPSIY VKFRSKEDPN FFIVIWTTTP WTLPANVGIA LHPDYEYSVV 250
KVGEEKWVIA TDLLDAFSKE TGIDCSNVVE KIKGKDLEGK EFVHPIFDDK 300
TSRVILADYV SLETGTGCVH IAPGHGEEDY IYGHVQYGLP IVSPVDEEGR 350
FTEEAGKYKG MFIEDANEVI IEDLKKKGIL VHASSITHSY PHCWRCKGPV 400
IFRATEQWFI SVDHNNLRQK VLEEIDKVKW IPEWGRNRIR SMVEERPDWC 450
ISRQRVWGTP IPAVKCKECG EVTLDPKVIE HFMKIVEKEG TNAWFEKEVE 500
ELIPEDFVCP KCGKRSFEKM LDTLDVWIDS GASFEYITTK REDHPFPLDM 550
YLEGSDQHRG WFHSSIFLAV AKRGSAPYKE VLTHGFIKDE QGRKMSKSLG 600
NVVDPMEVVE KYGAEILRLW LASSDYFNDI KISMRIVEQQ TEVYRKIRNT 650
FRFLLGNLEG FDPELDRVPY EKLLTIDRWA LGRLQEIIKR ATEYYDSYEF 700
SKVYNLVVKY CTTELSSLYL DVVKDRLYVE AKDSIYRRSA QTVMHEILIA 750
LMKILAPIMT FTMEEVYSHL HEKDRKYKTV QAEYWPEYKE EFIDRELMED 800
FEKLLSIRED VLKALEEKRQ QDVIGHSLDA EVVLVPKNDT IKALLEKYRD 850
ILEELFIVSK VSLSDASGEL KGELVEVTVK HAEGEKCQRC WKYTTEISTS 900
EDFPGVCPRC LAVLKGERK 919
Length:919
Mass (Da):107,044
Last modified:June 26, 2007 - v1
Checksum:iC80CC27CF8AA62DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000702 Genomic DNA. Translation: ABQ47435.1.
RefSeqiWP_011943889.1. NC_009486.1.
YP_001245011.1. NC_009486.1.

Genome annotation databases

EnsemblBacteriaiABQ47435; ABQ47435; Tpet_1422.
GeneIDi5171003.
KEGGitpt:Tpet_1422.
PATRICi23945691. VBIThePet65348_1442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000702 Genomic DNA. Translation: ABQ47435.1 .
RefSeqi WP_011943889.1. NC_009486.1.
YP_001245011.1. NC_009486.1.

3D structure databases

ProteinModelPortali A5IML2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 390874.Tpet_1422.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ47435 ; ABQ47435 ; Tpet_1422 .
GeneIDi 5171003.
KEGGi tpt:Tpet_1422.
PATRICi 23945691. VBIThePet65348_1442.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TPET390874:GHJI-1468-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Entry informationi

Entry nameiSYI_THEP1
AccessioniPrimary (citable) accession number: A5IML2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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