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A5IMI1 (ISPD_THEP1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
Gene names
Name:ispD
Ordered Locus Names:Tpet_1390
OrganismThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) [Complete proteome] [HAMAP]
Taxonomic identifier390874 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108

Sequence similarities

Belongs to the IspD family.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2222222-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
PRO_1000022956

Sites

Site141Transition state stabilizer By similarity
Site211Transition state stabilizer By similarity
Site1491Positions MEP for the nucleophilic attack By similarity
Site2011Positions MEP for the nucleophilic attack By similarity

Sequences

Sequence LengthMass (Da)Tools
A5IMI1 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: 4C1283E81078A9FD

FASTA22225,388
        10         20         30         40         50         60 
MNVAILLAAG KGERMSENVP KQFLEIEGRM LFEYPLSTFL KSEAIDGVVI VTRREWFEVV 

        70         80         90        100        110        120 
EKRVFHEKVL GIVEGGDTRS QSVRSALEFL EKFSPSYVLV HDSARPFLRK KHVSEVLRRA 

       130        140        150        160        170        180 
RETGAATLAL KNSDALVRVE NDRMEYIPRK GVYRILTPQA FSYEILKKAH ENGGEWADDT 

       190        200        210        220 
EPVQKLGVKI ALVEGDPLCF KVTFKEDLEL ARIIAREWER IP

« Hide

References

[1]"Complete sequence of Thermotoga petrophila RKU-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000702 Genomic DNA. Translation: ABQ47404.1.
RefSeqYP_001244980.1. NC_009486.1.

3D structure databases

ProteinModelPortalA5IMI1.
SMRA5IMI1. Positions 1-219.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5IMI1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5171469.
GenomeReviewsGene locus Tpet_1390 in contig CP000702_GR.
KEGGtpt:Tpet_1390.
PATRIC23945616. VBIThePet65348_1409.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAPSNIKVT.
ProtClustDBCLSK875736.

Enzyme and pathway databases

BioCycTPET390874:TPET_1390-MONOMER.

Family and domain databases

HAMAPMF_00108. IspD.
[Tree]
InterProIPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
[Graphical view]
KOK00991.
PfamPF01128. IspD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. IspD. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPD_THEP1
AccessionPrimary (citable) accession number: A5IMI1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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