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A5IK57

- BIOB_THEP1

UniProt

A5IK57 - BIOB_THEP1

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Protein

Biotin synthase

Gene

bioB

Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi176 – 1761Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi247 – 2471Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTPET390874:GHJI-574-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Tpet_0559
OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Taxonomic identifieri390874 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000006558: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Biotin synthasePRO_0000381685Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi390874.Tpet_0559.

Structurei

3D structure databases

ProteinModelPortaliA5IK57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5IK57-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKEILSME TKELILKANR MTSNLKLDLC SIVNAKSGIC DQDCKFCAQS
60 70 80 90 100
SLYNTGVKKY PLLDKESILE KAKEAEKMGA IRFGIVTSGK RLTRKEILKV
110 120 130 140 150
AKIIEFLKAN TNLKICASLG VLEKDELRYL KENGLDRYHH NLETSPGFFR
160 170 180 190 200
NICTTHTFYD RVKTVENAKN VELEVCSGGI FGVGENFDDR LELAKILKDL
210 220 230 240 250
EVDSVPINFL IPIRGTPFEN FQKLDVVERI RTIAMFRVVL GENVTIKIAA
260 270 280 290
GREDFGDFQA LAFFAGANGM IVGGYLTLKG RSYDDDIKLI EGLRELMRW
Length:299
Mass (Da):33,777
Last modified:June 26, 2007 - v1
Checksum:iA16B9D774A0ED6D0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000702 Genomic DNA. Translation: ABQ46580.1.
RefSeqiYP_001244156.1. NC_009486.1.

Genome annotation databases

EnsemblBacteriaiABQ46580; ABQ46580; Tpet_0559.
GeneIDi5170440.
KEGGitpt:Tpet_0559.
PATRICi23943878. VBIThePet65348_0565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000702 Genomic DNA. Translation: ABQ46580.1 .
RefSeqi YP_001244156.1. NC_009486.1.

3D structure databases

ProteinModelPortali A5IK57.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 390874.Tpet_0559.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ46580 ; ABQ46580 ; Tpet_0559 .
GeneIDi 5170440.
KEGGi tpt:Tpet_0559.
PATRICi 23943878. VBIThePet65348_0565.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci TPET390874:GHJI-574-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Entry informationi

Entry nameiBIOB_THEP1
AccessioniPrimary (citable) accession number: A5IK57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 26, 2007
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3