NH(3)-dependent NAD(+) synthetase - Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)

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A5IJE0 (A5IJE0_THEP1) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 31. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Tpet_0284

Organism

Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) [Complete proteome] [HAMAP]

Taxonomic identifier

390874 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 EMBL ABQ46313.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

24 – 31

ATP By similarity HAMAP MF_00193

Sites

Active site

By similarity HAMAP MF_00193

Sequences

Sequence

Length

Mass (Da)

Tools

A5IJE0 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: 1CE941E517A26739
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FASTA

281

32,385

        10         20         30         40         50         60 
MKESLISFIR EKIEEYNYKG AVVGVSGGID SAVVLSLCVQ ALGKDRVFGL ILPERDSSKD 

        70         80         90        100        110        120 
SVKDAVELCE SLNVEYKIKS ITPILRKIGV YRLFPPKLFF PESVVKRYVL NKWNSLSKDP 

       130        140        150        160        170        180 
FLDDLRNSGP DEFLKGLAYY RIKHRIRMCL LYFEAEKRGY AVVGTTNRTE YLTGLYVKWG 

       190        200        210        220        230        240 
DEAVDIEPIM HLYKTQVFEL ARELNIPEKI QKKPPSPDLI PGVTDEMAFG MSYIELDRIL 

       250        260        270        280 
MKIERKEDLS DEDPEKVERV KKILELAEKH RRGIPVTFDK I

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References

[1]

"Complete sequence of Thermotoga petrophila RKU-1."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.

Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000702 Genomic DNA. Translation: ABQ46313.1.
RefSeq	YP_001243889.1. NC_009486.1.
3D structure databases
ProteinModelPortal	A5IJE0.
ModBase	Search...
Protein-protein interaction databases
STRING	A5IJE0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5170573.
GenomeReviews	Gene locus Tpet_0284 in contig CP000702_GR.
KEGG	tpt:Tpet_0284.
PATRIC	23943293. VBIThePet65348_0284.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	CLSK875266.
Enzyme and pathway databases
BioCyc	TPET390874:TPET_0284-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 2 hits.
ProtoNet	Search...

Entry information

Entry name

A5IJE0_THEP1

Accession

Primary (citable) accession number: A5IJE0

Entry history

Integrated into UniProtKB/TrEMBL:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information