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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 126FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciTPET390874:GHJI-199-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Tpet_0195
OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Taxonomic identifieri390874 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000006558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000346417Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi390874.Tpet_0195.

Structurei

3D structure databases

ProteinModelPortaliA5IJ51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

A5IJ51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVNVIGAGL AGSEVAYNLG KRGIRVRLFE MRPKKMTEVH KTGYFAELVC
60 70 80 90 100
SNSLKSEDIT NAEGLLKAEM KLMGSITLEA AEKARVPSGK ALAVDRNIFA
110 120 130 140 150
KEVTEAIESL ESVEVIREEV TEFDPEEGLW VVATGPATSD GLFSFLRNLL
160 170 180 190 200
GDDFLFFFDA VSPIVTFESI DMEHAFWGDR FGKGKDYINC PLTDGEYEEL
210 220 230 240 250
WKALVEAEVI EMEDFDRKLL FERCQPIEEI ARSGKDALRY GPLRPTGLVD
260 270 280 290 300
PRTGKEPYAV VQLRREDKEG RFYSLVGFQT RLKWNEQKRV LRKIPCLRNA
310 320 330 340 350
EIVRYGVMHR NVYINSPKLL DIFFRLKKQP NIFFAGQITG VEGYMESAAS
360 370 380 390 400
GIYVAYNVYR ILKGLSPLKL PEETMMGALF SYIIEKVEGD LKPMYANFGL
410 420 430
LPPLKTRVKD KFGKRKKLAE RAIEAMRKFL EEYPW
Length:435
Mass (Da):49,557
Last modified:June 26, 2007 - v1
Checksum:i969BD5B076202902
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ46224.1.
RefSeqiWP_011942878.1. NC_009486.1.

Genome annotation databases

EnsemblBacteriaiABQ46224; ABQ46224; Tpet_0195.
KEGGitpt:Tpet_0195.
PATRICi23943115. VBIThePet65348_0195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ46224.1.
RefSeqiWP_011942878.1. NC_009486.1.

3D structure databases

ProteinModelPortaliA5IJ51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390874.Tpet_0195.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ46224; ABQ46224; Tpet_0195.
KEGGitpt:Tpet_0195.
PATRICi23943115. VBIThePet65348_0195.

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciTPET390874:GHJI-199-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Entry informationi

Entry nameiTRMFO_THEP1
AccessioniPrimary (citable) accession number: A5IJ51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 26, 2007
Last modified: May 27, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.