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A5IJ51 (TRMFO_THEP1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:Tpet_0195
OrganismThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) [Complete proteome] [HAMAP]
Taxonomic identifier390874 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 435435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000346417

Regions

Nucleotide binding7 – 126FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A5IJ51 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: 969BD5B076202902

FASTA43549,557
        10         20         30         40         50         60 
MIVNVIGAGL AGSEVAYNLG KRGIRVRLFE MRPKKMTEVH KTGYFAELVC SNSLKSEDIT 

        70         80         90        100        110        120 
NAEGLLKAEM KLMGSITLEA AEKARVPSGK ALAVDRNIFA KEVTEAIESL ESVEVIREEV 

       130        140        150        160        170        180 
TEFDPEEGLW VVATGPATSD GLFSFLRNLL GDDFLFFFDA VSPIVTFESI DMEHAFWGDR 

       190        200        210        220        230        240 
FGKGKDYINC PLTDGEYEEL WKALVEAEVI EMEDFDRKLL FERCQPIEEI ARSGKDALRY 

       250        260        270        280        290        300 
GPLRPTGLVD PRTGKEPYAV VQLRREDKEG RFYSLVGFQT RLKWNEQKRV LRKIPCLRNA 

       310        320        330        340        350        360 
EIVRYGVMHR NVYINSPKLL DIFFRLKKQP NIFFAGQITG VEGYMESAAS GIYVAYNVYR 

       370        380        390        400        410        420 
ILKGLSPLKL PEETMMGALF SYIIEKVEGD LKPMYANFGL LPPLKTRVKD KFGKRKKLAE 

       430 
RAIEAMRKFL EEYPW 

« Hide

References

[1]"Complete sequence of Thermotoga petrophila RKU-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000702 Genomic DNA. Translation: ABQ46224.1.
RefSeqYP_001243800.1. NC_009486.1.

3D structure databases

ProteinModelPortalA5IJ51.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390874.Tpet_0195.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ46224; ABQ46224; Tpet_0195.
GeneID5170571.
KEGGtpt:Tpet_0195.
PATRIC23943115. VBIThePet65348_0195.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMARFAGQIT.
OrthoDBEOG6J74VT.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycTPET390874:GHJI-199-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMFO_THEP1
AccessionPrimary (citable) accession number: A5IJ51
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families