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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Thioimide intermediateUniRule annotation
Active sitei52 – 521Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciTPET390874:GHJI-141-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:Tpet_0138
OrganismiThermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
Taxonomic identifieri390874 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000006558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 137137NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000062414Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi390874.Tpet_0138.

Structurei

3D structure databases

ProteinModelPortaliA5IIZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 703Substrate bindingUniRule annotation
Regioni87 – 882Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410816Z. Bacteria.
COG0780. LUCA.
HOGENOMiHOG000009528.
KOiK09457.
OMAiTSVCPMT.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00818. QueF_type1.
InterProiIPR029500. QueF.
IPR016856. QueF_type1.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
[Graphical view]
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.

Sequencei

Sequence statusi: Complete.

A5IIZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKAEGRIFD FKGHDAIRTD FLEAIDFDGK DEYIKIETDE FSAVCPFSGL
60 70 80 90 100
PDIGRVIIEY YPDGGKIVEL KSLKYYFVSF RNVGIYQEEA TKRIYEDLKN
110 120 130
LLKTDRIRVT VIYNIRGGIK TTTQMGSLEG KKSGEVE
Length:137
Mass (Da):15,675
Last modified:June 26, 2007 - v1
Checksum:i597073A219ECF27C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ46167.1.
RefSeqiWP_004080885.1. NC_009486.1.

Genome annotation databases

EnsemblBacteriaiABQ46167; ABQ46167; Tpet_0138.
KEGGitpt:Tpet_0138.
PATRICi23943001. VBIThePet65348_0139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000702 Genomic DNA. Translation: ABQ46167.1.
RefSeqiWP_004080885.1. NC_009486.1.

3D structure databases

ProteinModelPortaliA5IIZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390874.Tpet_0138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ46167; ABQ46167; Tpet_0138.
KEGGitpt:Tpet_0138.
PATRICi23943001. VBIThePet65348_0139.

Phylogenomic databases

eggNOGiENOG410816Z. Bacteria.
COG0780. LUCA.
HOGENOMiHOG000009528.
KOiK09457.
OMAiTSVCPMT.
OrthoDBiEOG6D5G0M.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciTPET390874:GHJI-141-MONOMER.

Family and domain databases

HAMAPiMF_00818. QueF_type1.
InterProiIPR029500. QueF.
IPR016856. QueF_type1.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
[Graphical view]
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RKU-1 / ATCC BAA-488 / DSM 13995.

Entry informationi

Entry nameiQUEF_THEP1
AccessioniPrimary (citable) accession number: A5IIZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 26, 2007
Last modified: December 9, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.