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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Legionella pneumophila (strain Corby)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei559Aminoacyl-adenylateUniRule annotation1
Binding sitei603ATPUniRule annotation1
Metal bindingi894ZincUniRule annotation1
Metal bindingi897ZincUniRule annotation1
Metal bindingi914ZincUniRule annotation1
Metal bindingi917ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LPC_2353
OrganismiLegionella pneumophila (strain Corby)
Taxonomic identifieri400673 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220871 – 931Isoleucine--tRNA ligaseAdd BLAST931

Proteomic databases

PRIDEiA5IFX5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5IFX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi600 – 604"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A5IFX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYKDTLNL PNTSFPMKAS LSVREPEMLA DWQAKGIYQK IRKARVGSKR
60 70 80 90 100
FILHDGPPYA NGHLHCGHAL NKILKDIIIK SKTFSGFDAP FVPGWDCHGL
110 120 130 140 150
PIELNVEKKV GKAGSKISPR EFRAKCREYA ASQIDIQRDE FQRLGVLGDW
160 170 180 190 200
YNPYVTMDYH YEANIVRALG LMIKNGHLQQ GFKPVHWCID CGSALAEAEV
210 220 230 240 250
DYEDKTSPSI DVAFSAVNPS EFLNCFETQP AVKPLILPIW TTTPWTLPAN
260 270 280 290 300
EAVCLHPEID YALIDAGNSY YIVATDLVES VMARYGISHY KTSGFAKGRV
310 320 330 340 350
FEHFKLQHPF YKRQVPVVLA EHVTTESGTG CVHTAPAHGP DDYLVGQSYQ
360 370 380 390 400
LPLINPVMAN GCFAENVELF AGISVLKANE TILAVLSERN VLLASESIRH
410 420 430 440 450
SYPHCWRHKS PMIFLATPQW FISMDKSNLR QAIINEIDKV NWVPDWGKAR
460 470 480 490 500
ISNMVENRPD WCISRQRSWG TPMPLFVHKT TRELHPDTLE LIERVAVMIE
510 520 530 540 550
KSGIDAWFDL DSSELLGDDA KHYDKITDTM DVWLDSGISH YSVLKHNNDL
560 570 580 590 600
DFPADVYFEG SDQHRGWFNS SLTTAVAMYG VAPYKTVLTH GYTVDAEGKK
610 620 630 640 650
LSKSKGNYVA LDKLVNQHGA DILRLWVAST DYRHEVSISE EIIKRNADAY
660 670 680 690 700
RRIRNTARFL LANLFDFNPA SDCIDAKELL ELDRWALKRC QLLQEEIITA
710 720 730 740 750
YENYHFHLIY QKIHNFCAVD MGSFYLDLIK DRQYTTAKDS IARRSCQTAM
760 770 780 790 800
YHMVKAFTIW LAPILSFTAE EIWQTIPGNN SESIFIEHWY NAWPTIDAVN
810 820 830 840 850
MEDWEQLHIV RDEVNKALEE TRQRGEIGSA LAAEVTVYAD AKVLPKLTRL
860 870 880 890 900
GEELRFLFIT SEAKACPISQ SPKGLAVTDC GVSIQVTASV HEKCARCWHR
910 920 930
REDVGQNQEH PELCLRCVGN ISRYHEERLY I
Length:931
Mass (Da):105,933
Last modified:June 26, 2007 - v1
Checksum:iE02C6D9F6C2A3DFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000675 Genomic DNA Translation: ABQ56275.1
RefSeqiWP_011946026.1, NC_009494.2

Genome annotation databases

EnsemblBacteriaiABQ56275; ABQ56275; LPC_2353
KEGGilpc:LPC_2353

Similar proteinsi

Entry informationi

Entry nameiSYI_LEGPC
AccessioniPrimary (citable) accession number: A5IFX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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