Reviewed, UniProtKB/Swiss-Prot A5IF70 (KATG1_LEGPC)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	LPC_2090

Organism

Legionella pneumophila (strain Corby) [Complete proteome] [HAMAP]

Taxonomic identifier

400673 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. HAMAP MF_01961
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354816
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5IF70-1 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: DE864894F5075A59	FASTA	721	80,488
	10 20 30 40 50 60 MDGKVGNTTT GCPVMHGGMT SAGTSNTAWW PNALNLDILH QHDTKTNPMG KDFNYREEVK 70 80 90 100 110 120 KLNFEALKKD LHALMTDSQA WWPADWGHYG GLMIRMSWHA AGSYRVADGR GGAGTGNQRF 130 140 150 160 170 180 APLNSWPDNV NLDKARRLLW PIKKKYGNKI SWADLIVLAG TIAYESMGLK TFGFGFGRED 190 200 210 220 230 240 IWHPEKDVYW GSEQEWLGAK RYDDKDRQSL ETPLAAVQMG LIYVNPEGVN GQPDPLRTAQ 250 260 270 280 290 300 DVRVTFGRMA MNDEETVALT AGGHTVGKCH GNGNAKFLGP EPEAADIEDQ GLGWINKTTR 310 320 330 340 350 360 GIGRNTVSSG IEGAWTTHPT QWDNGYFYLL LNYDWELKKS PAGAWQWEPI HIKEEDKPVD 370 380 390 400 410 420 VEDPAIRHNP IMTDADMAIK MDPVYRKIAE RFYKDPDYFA EVFARAWFKL THRDMGPKTR 430 440 450 460 470 480 YIGPDVPKED LIWQDPVPAG NRAYDIAAAK AKIAASNLTI GEMVSTAWDS ARTFRGSDKR 490 500 510 520 530 540 GGANGARIRL KPQKDWEGNE PQRLTKVLRI LEDIAADTGA SVADVIVLAG NVGIEKAAKA 550 560 570 580 590 600 AGFDIIVPFA PGRGDATDDM TDAESFDVLE PLHDGYRNWL KKAYDVRPEE LMLDRTQLMG 610 620 630 640 650 660 LTAHEMTVLV GGLRVLGTNH NNTQHGVFTD RVGALTNDFF VNLTDMANVW IPSKDNLYEI 670 680 690 700 710 720 RDRKAGNIKW TATRVDLVFG SNSILRSYAE VYAQDDNKGK FIQDFVAAWT KVMNADRFDL A

References

[1]

"Identification and characterization of a new conjugation/ type IVA secretion system (trb/tra) of L. pneumophila Corby localized on a mobile genomic island." Gloeckner G., Albert-Weissenberger C., Weinmann E., Jacobi S., Schunder E., Steinert M., Buchrieser C., Hacker J., Heuner K. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000675 Genomic DNA. Translation: ABQ56020.1.
RefSeq	YP_001251366.1.
3D structure databases
SMR	A5IF70. Positions 25-720.
ModBase	Search...
Protein-protein interaction databases
STRING	A5IF70.
Genome annotation databases
GeneID	5179928.
GenomeReviews	Gene locus LPC_2090 in contig CP000675_GR.
KEGG	lpc:LPC_2090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_LEGPC

Accession

Primary (citable) accession number: A5IF70

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 26, 2007
Last modified:	February 9, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)