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A5ICM0

- GLND_LEGPC

UniProt

A5ICM0 - GLND_LEGPC

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Legionella pneumophila (strain Corby)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciLPNE400673:GCIT-1827-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:LPC_1154
OrganismiLegionella pneumophila (strain Corby)
Taxonomic identifieri400673 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
ProteomesiUP000001569: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022345Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi400673.LPC_1154.

Structurei

3D structure databases

ProteinModelPortaliA5ICM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini441 – 570130HDUniRule annotationAdd
BLAST
Domaini679 – 76082ACT 1UniRule annotationAdd
BLAST
Domaini788 – 86174ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 321321UridylyltransferaseAdd
BLAST
Regioni322 – 678357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR005105. GlnD_Uridyltrans_N.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF03445. DUF294. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5ICM0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNDNRIIKN TIKQFKEKLC KDFSQKANIT SITRKLAVFI DTILIQLFIK
60 70 80 90 100
NKLHFGDNFC LLALGSYGRR ELQLHSDIDL LILHTEKVSN IQLQRAQKFI
110 120 130 140 150
QDCWDVGLEV SHQITTVSSC ANLASQDLSV ISTIMDMFLL CGHGALMEEL
160 170 180 190 200
IYQTHTLHMW PSHQYFFAKL QEQQNRYAKY GETAYNLEPN IKNGPGGLRD
210 220 230 240 250
LQILLSISKR HFKIKKLAEG IGYGFITDKE YEELKYCQNF LWRVRFALHM
260 270 280 290 300
LAGKPEERLS FDYQVKLAQF FGYQDQSHIL AIEQFMKDYF KVIKRNRELN
310 320 330 340 350
EMLLQWFNET IVYHQKQKII RLDDEFQLSN RFIEVRNNRV FKQNPQSILK
360 370 380 390 400
LFYWLVKRPD IEGVRASTIR LIRESLFLMG KRFRESKETA NIFINIFRTG
410 420 430 440 450
NDPYDALQRM NRYGVLAHYL DCFATATGQM QYDLFHAYTV DQHTLFVIRN
460 470 480 490 500
ISRFKKNEYA KQFPLCAKII TALEKPEILY LGALFHDIAK GRGGDHSELG
510 520 530 540 550
AIEAQQFTQR HYMEAEDSKL IVWLVRYHLL MSQTAQRKDI YDPKTIEQFC
560 570 580 590 600
QLLPHARYLD YLYLLTVADI CGTNPTLWNA WKDSLLKELY HAAKTRLHKQ
610 620 630 640 650
QELLDEAALI SIRKQYAMDI LISDGISSRV IQDLWSQFKG KYFLHESPEV
660 670 680 690 700
IARHTKAILN SKQFPVVIIM PHHSQGGTEV FIYMPHKDER FTITTSVLSN
710 720 730 740 750
HHVTIQEAAI ITCDNQFDLD TYIILDENNQ AFLNEQRARD IQKNLCDHLA
760 770 780 790 800
NTGRLPAVSR RRLSRALTHF NVKTQINFID DNTNHQTQLF LVTNDRPGLL
810 820 830 840 850
ATISRVFLTL NIHLHNAKIA TAGERVEDMF YISNQTGYSL NHEEKTILKE
860
KLILEISKSK Y
Length:861
Mass (Da):100,711
Last modified:June 26, 2007 - v1
Checksum:i5D4C195868AFC94F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000675 Genomic DNA. Translation: ABQ55120.1.
RefSeqiYP_001250466.1. NC_009494.2.

Genome annotation databases

EnsemblBacteriaiABQ55120; ABQ55120; LPC_1154.
GeneIDi5180977.
KEGGilpc:LPC_1154.
PATRICi22308727. VBILegPne45588_1196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000675 Genomic DNA. Translation: ABQ55120.1 .
RefSeqi YP_001250466.1. NC_009494.2.

3D structure databases

ProteinModelPortali A5ICM0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 400673.LPC_1154.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ55120 ; ABQ55120 ; LPC_1154 .
GeneIDi 5180977.
KEGGi lpc:LPC_1154.
PATRICi 22308727. VBILegPne45588_1196.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci LPNE400673:GCIT-1827-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR005105. GlnD_Uridyltrans_N.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF03445. DUF294. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification and characterization of a new conjugation/ type IVA secretion system (trb/tra) of L. pneumophila Corby localized on a mobile genomic island."
    Gloeckner G., Albert-Weissenberger C., Weinmann E., Jacobi S., Schunder E., Steinert M., Buchrieser C., Hacker J., Heuner K.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Corby.

Entry informationi

Entry nameiGLND_LEGPC
AccessioniPrimary (citable) accession number: A5ICM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3