Reviewed, UniProtKB/Swiss-Prot A5IBB3 (HEM6_LEGPC)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	LPC_0684

Organism

Legionella pneumophila (strain Corby) [Complete proteome] [HAMAP]

Taxonomic identifier

400673 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019472
Regions
	Region	56 – 65	10	Important for dimerization By similarity
	Region	116 – 118	3	Substrate binding By similarity
	Region	248 – 283	36	Important for dimerization By similarity
	Region	266 – 271	6	Substrate binding By similarity
Sites
	Active site	114	1	Proton donor By similarity
	Binding site	100	1	Substrate By similarity
	Site	183	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5IBB3-1 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: 24DA5D277CAC0B90	FASTA	311	35,820
	10 20 30 40 50 60 MPISKTLPYN AIEQIKSYLL QLQNTICVSL ESIDGKTRFH EDSWQRAAGG GGKTRIMANG 70 80 90 100 110 120 NVFEKAGVNF SHVSGEQLPA SASAHREELA GRHFSALGVS LVIHPQNPYV PTTHANVRFF 130 140 150 160 170 180 VAEKEDSEPV WWFGGGFDLT PYYGFVEDCE HWHQTALNAC LPFGETIYPK FKRWCDDYFF 190 200 210 220 230 240 IKHRNEARGI GGLFFDDYNE ISFDHSFELM RSIGDHFILA YEPIVARRKD IPFGNREKAF 250 260 270 280 290 300 QNYRRGRYAE FNLVYDRGTL FGLQSGGRTE SILMSLPPIV HWEYNWHPEK GSDEEKLYTD 310 FLPAKDWLKK E

References

[1]

"Identification and characterization of a new conjugation/ type IVA secretion system (trb/tra) of L. pneumophila Corby localized on a mobile genomic island." Gloeckner G., Albert-Weissenberger C., Weinmann E., Jacobi S., Schunder E., Steinert M., Buchrieser C., Hacker J., Heuner K. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000675 Genomic DNA. Translation: ABQ54663.1.
RefSeq	YP_001250009.1.
3D structure databases
SMR	A5IBB3. Positions 11-308.
ModBase	Search...
Protein-protein interaction databases
STRING	A5IBB3.
Genome annotation databases
GeneID	5180516.
GenomeReviews	Gene locus LPC_0684 in contig CP000675_GR.
KEGG	lpc:LPC_0684.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	HMNTRFV.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_LEGPC

Accession

Primary (citable) accession number: A5IBB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 26, 2007
Last modified:	January 19, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)