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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Legionella pneumophila (strain Corby)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521Manganese 2UniRule annotation
Metal bindingi257 – 2571Manganese 1UniRule annotation
Metal bindingi257 – 2571Manganese 2UniRule annotation
Active sitei264 – 2641UniRule annotation
Metal bindingi275 – 2751Manganese 2UniRule annotation
Metal bindingi334 – 3341Manganese 1UniRule annotation
Metal bindingi336 – 3361Manganese 1UniRule annotation
Metal bindingi336 – 3361Manganese 2UniRule annotation
Active sitei338 – 3381UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciLPNE400673:GCIT-2852-MONOMER.

Protein family/group databases

MEROPSiM17.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:LPC_0510
OrganismiLegionella pneumophila (strain Corby)
Taxonomic identifieri400673 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
ProteomesiUP000001569: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Probable cytosol aminopeptidasePRO_1000203833Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi400673.LPC_0510.

Structurei

3D structure databases

ProteinModelPortaliA5IAU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243132.
KOiK01255.
OMAiMPANICT.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5IAU5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYGLTHTPS LTMSECLVLG VFSDLALPDF ATAIDNEQKG LIKKLCQRVP
60 70 80 90 100
EPGNTVWQTD VEGHSLLIIQ CGKKEEFSAN SLQKRVGEIT EALIKQRFSS
110 120 130 140 150
ATVCLPRLNQ ESAEWQLEQM IVQIDNLRYQ LLDFKTKHAK SHKLESVIFH
160 170 180 190 200
LPGATEKSLE MAKAIVTGVE FCRDLANMPA NICTPTYLGE QAISLSKQFD
210 220 230 240 250
QISCQVMGPE EIKEMGMGAL LAVAQGSDQP PRLIDIHYHG NKNSTPVILV
260 270 280 290 300
GKGITFDSGG LSIKPANAMD EMKYDMSGAA SVLGVIKACA LLKLPINLIG
310 320 330 340 350
IIASAENLIS GSAVKSGDIV TTMSGQTVEI INTDAEGRLV LADALTYAER
360 370 380 390 400
YNPDFVIDIA TLTGAIIVAL GNIATGYMTR DEQLAKSIER AANESQDKVW
410 420 430 440 450
RMPLDEAYQD ALESPLADMI NAGFDRSAGS ITAACFLSRF TEKYRWAHLD
460 470 480
IAGTAWISGK KRNATGRPVP LLIQLLRHVA NSR
Length:483
Mass (Da):52,613
Last modified:June 26, 2007 - v1
Checksum:i695CC2F46D38ACE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000675 Genomic DNA. Translation: ABQ54495.1.
RefSeqiWP_011947549.1. NC_009494.2.
YP_001249841.1. NC_009494.2.

Genome annotation databases

EnsemblBacteriaiABQ54495; ABQ54495; LPC_0510.
GeneIDi5182305.
KEGGilpc:LPC_0510.
PATRICi22307385. VBILegPne45588_0530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000675 Genomic DNA. Translation: ABQ54495.1.
RefSeqiWP_011947549.1. NC_009494.2.
YP_001249841.1. NC_009494.2.

3D structure databases

ProteinModelPortaliA5IAU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi400673.LPC_0510.

Protein family/group databases

MEROPSiM17.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ54495; ABQ54495; LPC_0510.
GeneIDi5182305.
KEGGilpc:LPC_0510.
PATRICi22307385. VBILegPne45588_0530.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243132.
KOiK01255.
OMAiMPANICT.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciLPNE400673:GCIT-2852-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and characterization of a new conjugation/ type IVA secretion system (trb/tra) of L. pneumophila Corby localized on a mobile genomic island."
    Gloeckner G., Albert-Weissenberger C., Weinmann E., Jacobi S., Schunder E., Steinert M., Buchrieser C., Hacker J., Heuner K.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Corby.

Entry informationi

Entry nameiAMPA_LEGPC
AccessioniPrimary (citable) accession number: A5IAU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 26, 2007
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.