A5I7T7 (MURI_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate racemase
EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	CBO3562, CLC_3542

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity. HAMAP MF_00258
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Glutamate racemase HAMAP MF_00258		PRO_1000047557

Sequences

	Sequence		Length	Mass (Da)
	A5I7T7 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: CFE981168CA1CD5B	FASTA	257	28,741
	10 20 30 40 50 60 MSINDKPIGF FDSGVGGISV LKEAFKLLPK EDFLYYGDSK NAPYGTKKVE EVKALTFNAT 70 80 90 100 110 120 DFLMSKGIKA LVVACNTATS VTINDLRENY DIPIIGIEPA LKPAVELKKG GKIIIMATPM 130 140 150 160 170 180 TLAEKKFANL MDLYKETEDI EPLPCPGLPE LIEQGIVSGD VIYNYLKDKF SKYDNEKISS 190 200 210 220 230 240 IVLGCTHYPF IEETLKEVTH NKACIIDGSF GTSRELKRQL KNSNMLTEEN RVGKVTIFNS 250 REDKDIIDLS YKLFNMK

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS39097.1. AM412317 Genomic DNA. Translation: CAL85122.1.
RefSeq	YP_001256043.1. NC_009495.1. YP_001389284.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5I7T7.
ModBase	Search...
Protein-protein interaction databases
STRING	A5I7T7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5187853. 5398350.
GenomeReviews	Gene locus CBO3562 in contig AM412317_GR. Gene locus CLC_3542 in contig CP000727_GR.
KEGG	cbh:CLC_3542. cbo:CBO3562.
PATRIC	19369289. VBICloBot22612_3539.
Phylogenomic databases
eggNOG	COG0796.
HOGENOM	HBG645102.
OMA	QCHVRLV.
ProtClustDB	PRK00865.
Enzyme and pathway databases
BioCyc	CBOT36826:CBO3562-MONOMER. CBOT441771:CLC_3542-MONOMER.
Family and domain databases
HAMAP	MF_00258. Glu_racemase. [Tree]
InterPro	IPR015942. Asp/Glu/hydantoin_racemase. IPR001920. Asp/Glu_race. IPR018187. Asp/Glu_racemase_AS. IPR004391. Glu_race. [Graphical view]
Gene3D	G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.
KO	K01776.
PANTHER	PTHR21198. PTHR21198. 1 hit.
Pfam	PF01177. Asp_Glu_race. 1 hit. [Graphical view]
SUPFAM	SSF53681. Asp/Glu_race. 2 hits.
TIGRFAMs	TIGR00067. Glut_race. 1 hit.
PROSITE	PS00923. ASP_GLU_RACEMASE_1. 1 hit. PS00924. ASP_GLU_RACEMASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURI_CLOBH

Accession

Primary (citable) accession number: A5I7T7
Secondary accession number(s): A7G919

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 26, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program