A5I7K8 (EFTU_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf1
Synonyms:	tufA
Ordered Locus Names:	CBO3482, CLC_3427
AND
Name:	tuf2
Synonyms:	tufB
Ordered Locus Names:	CBO3496, CLC_3441

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Reference proteome] [HAMAP]

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum A

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Elongation factor Tu HAMAP-Rule MF_00118		PRO_0000337357
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5I7K8 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: 75AD3C5B65D90FA8	FASTA	397	43,483
	10 20 30 40 50 60 MAKAKFERSK PHVNIGTIGH VDHGKTTLTA AITTVLAQKG GASATKYDEI DKAPEEKERG 70 80 90 100 110 120 ITINTSHVEY ETANRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLASRVGVQY IVVFLNKADQ VDDPELIELV EMEVRELLNE YGFPGDDTPI VVGSALEVLE 190 200 210 220 230 240 NQDNAEKTKC IDELMEAIDS YIPTPERATD QPFLMPVEDV FTITGRGTVA TGRVERGVLH 250 260 270 280 290 300 TGDEVELIGM KQEVSKTVCT GIEMFRKILD EAMAGDNIGA LLRGIQRDEI QRGQVLAKPG 310 320 330 340 350 360 SVTPHKKFVG QVYVLKKEEG GRHTPFFNGY RPQFYFRTTD VTGSINLPEG VEMVMPGDHI 370 380 390 DMAVELITPV AMHENLRFAI REGGRTVGSG VVTTISE

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS37793.1. CP000727 Genomic DNA. Translation: ABS39226.1. AM412317 Genomic DNA. Translation: CAL85043.1. AM412317 Genomic DNA. Translation: CAL85057.1.
RefSeq	YP_001255964.1. NC_009495.1. YP_001255978.1. NC_009495.1. YP_001389205.1. NC_009698.1. YP_001389219.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5I7K8.
SMR	A5I7K8. Positions 2-397.
ModBase	Search...
Protein-protein interaction databases
STRING	413999.CBO3496.
Proteomic databases
PRIDE	A5I7K8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS37793; ABS37793; CLC_3441. ABS39226; ABS39226; CLC_3427.
GeneID	5187735. 5187740. 5401095. 5401647.
KEGG	cbh:CLC_3427. cbh:CLC_3441. cbo:CBO3482. cbo:CBO3496.
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	THVEYES.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	CBOT413999:GJ72-3616-MONOMER. CBOT413999:GJ72-3630-MONOMER. CBOT441771:GIWX-3374-MONOMER. CBOT441771:GIWX-3388-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_CLOBH

Accession

Primary (citable) accession number: A5I7K8
Secondary accession number(s): A7G8U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program