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A5I6G4

- PBPA_CLOBH

UniProt

A5I6G4 - PBPA_CLOBH

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Protein
Penicillin-binding protein 1A
Gene
pbpA, CBO3083, CLC_2985
Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei398 – 3981Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro
  2. peptidase activity Source: InterPro
  3. transferase activity, transferring pentosyl groups Source: InterPro

GO - Biological processi

  1. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  2. regulation of cell shape Source: UniProtKB-KW
  3. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-3183-MONOMER.
CBOT441771:GIWX-2948-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:pbpA
Ordered Locus Names:CBO3083, CLC_2985
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini40 – 830791Extracellular Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Penicillin-binding protein 1A
PRO_5000246800Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO3083.

Structurei

3D structure databases

ProteinModelPortaliA5I6G4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 229173Transglycosylase By similarity
Add
BLAST
Regioni357 – 641285Transpeptidase By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 97Poly-Lys
Compositional biasi764 – 82865Asn-rich
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.
In the C-terminal section; belongs to the transpeptidase family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0744.
HOGENOMiHOG000041137.
KOiK05366.
OMAiFLSHQRT.
OrthoDBiEOG6KDKQS.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

A5I6G4-1 [UniParc]FASTAAdd to Basket

« Hide

MGKKKKKRKS SAFKIILNVF LSIFLVAGVA FGGIVFAMIK TAPPLNVQQV    50
LTFDEPSILY DDKGQYMDKV ITNEQRIVVD YKNVPQNLKN AFVSIEDERF 100
YKHHGVDIKR FTGVILINVT NKIKRSSKLQ GASTLTQQLI KNTVLSSEVS 150
IKRKVQEMYL SIQLEKELSK DEILGAYMNS IFLGGNALGV EAASKQYFNK 200
SVKDLSLIEC AFIAGVPQSP SVYYPYSSAS KKNPSIYLNR TKTVLYKMLD 250
NGYITQNDYN KALKDLDSKK LVFAKPSAPS NKLAYEWFSI PAIEQVKKDL 300
KTQYKYDDKQ IHNLLVNGGL KVYTTMNKNL QDKTQNTINN AYYLNSYKSN 350
GMIYPQASAV IMDYHNGEVK TIIGGRGDQP ARSYNRAASY NYLRPAGSSI 400
KPLTVYSAAI DSKKATAATG FEDSPIPNNI GRKYSSGAPY NPKNSPDIYY 450
GYVNVREALM RSINVVAVKL VDKIGLNTSI QYAEKFGIPI DQHDRSSIAS 500
LSLGELHKGT NPLIMAQAYG VFGNNGTYTE AKLYTKVVDR TGKVLLEPKT 550
NTKKVLSPEA AFITYDMLQG PVSESGTGPQ ANFGNMEVRG KTGTSSDMKN 600
LWFCGLTPYY SAAVWIGNDN SSTVDGVYSS TAARLWGDIM KEFHVNLPYK 650
QVQKPASVVT ANVDRISGKL PTQLSYRDPR GSTVYNEFFI NGTIPTEYDD 700
IHVEAQINKL TGKLASKFTP SFLVESRVFL RRDYSPGVEL LDQQWLLPYS 750
IDEGGSLPPT EEKNNSNTRD KNKDKNKNKN KDKNPSQDKP NNNNNDNNSN 800
NNNNNNDNNN NTKPPENDSN QNHEDNKNKQ 830
Length:830
Mass (Da):92,704
Last modified:June 26, 2007 - v1
Checksum:iDF9752C616B6D2C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS36996.1.
AM412317 Genomic DNA. Translation: CAL84646.1.
RefSeqiWP_012048098.1. NC_009698.1.
YP_001255575.1. NC_009495.1.
YP_001388812.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36996; ABS36996; CLC_2985.
GeneIDi5185759.
5401037.
KEGGicbh:CLC_2985.
cbo:CBO3083.
PATRICi19368243. VBICloBot22612_3061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS36996.1 .
AM412317 Genomic DNA. Translation: CAL84646.1 .
RefSeqi WP_012048098.1. NC_009698.1.
YP_001255575.1. NC_009495.1.
YP_001388812.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5I6G4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 413999.CBO3083.

Protein family/group databases

CAZyi GT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS36996 ; ABS36996 ; CLC_2985 .
GeneIDi 5185759.
5401037.
KEGGi cbh:CLC_2985.
cbo:CBO3083.
PATRICi 19368243. VBICloBot22612_3061.

Phylogenomic databases

eggNOGi COG0744.
HOGENOMi HOG000041137.
KOi K05366.
OMAi FLSHQRT.
OrthoDBi EOG6KDKQS.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci CBOT413999:GJ72-3183-MONOMER.
CBOT441771:GIWX-2948-MONOMER.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsi TIGR02074. PBP_1a_fam. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiPBPA_CLOBH
AccessioniPrimary (citable) accession number: A5I6G4
Secondary accession number(s): A7G7P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 26, 2007
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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