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A5I6G4 (PBPA_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Penicillin-binding protein 1A
Short name=PBP1a

Including the following 2 domains:

  1. Penicillin-insensitive transglycosylase
    EC=2.4.2.-
    Alternative name(s):
    Peptidoglycan TGase
  2. Penicillin-sensitive transpeptidase
    EC=3.4.-.-
    Alternative name(s):
    DD-transpeptidase
Gene names
Name:pbpA
Ordered Locus Names:CBO3083, CLC_2985
OrganismClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Reference proteome] [HAMAP]
Taxonomic identifier441771 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 51 family.

In the C-terminal section; belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 830830Penicillin-binding protein 1A
PRO_5000246800

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 3921Helical; Signal-anchor for type II membrane protein; Potential
Topological domain40 – 830791Extracellular Potential
Region57 – 229173Transglycosylase By similarity
Region357 – 641285Transpeptidase By similarity
Compositional bias3 – 97Poly-Lys
Compositional bias764 – 82865Asn-rich

Sites

Active site3981Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5I6G4 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: DF9752C616B6D2C1

FASTA83092,704
        10         20         30         40         50         60 
MGKKKKKRKS SAFKIILNVF LSIFLVAGVA FGGIVFAMIK TAPPLNVQQV LTFDEPSILY 

        70         80         90        100        110        120 
DDKGQYMDKV ITNEQRIVVD YKNVPQNLKN AFVSIEDERF YKHHGVDIKR FTGVILINVT 

       130        140        150        160        170        180 
NKIKRSSKLQ GASTLTQQLI KNTVLSSEVS IKRKVQEMYL SIQLEKELSK DEILGAYMNS 

       190        200        210        220        230        240 
IFLGGNALGV EAASKQYFNK SVKDLSLIEC AFIAGVPQSP SVYYPYSSAS KKNPSIYLNR 

       250        260        270        280        290        300 
TKTVLYKMLD NGYITQNDYN KALKDLDSKK LVFAKPSAPS NKLAYEWFSI PAIEQVKKDL 

       310        320        330        340        350        360 
KTQYKYDDKQ IHNLLVNGGL KVYTTMNKNL QDKTQNTINN AYYLNSYKSN GMIYPQASAV 

       370        380        390        400        410        420 
IMDYHNGEVK TIIGGRGDQP ARSYNRAASY NYLRPAGSSI KPLTVYSAAI DSKKATAATG 

       430        440        450        460        470        480 
FEDSPIPNNI GRKYSSGAPY NPKNSPDIYY GYVNVREALM RSINVVAVKL VDKIGLNTSI 

       490        500        510        520        530        540 
QYAEKFGIPI DQHDRSSIAS LSLGELHKGT NPLIMAQAYG VFGNNGTYTE AKLYTKVVDR 

       550        560        570        580        590        600 
TGKVLLEPKT NTKKVLSPEA AFITYDMLQG PVSESGTGPQ ANFGNMEVRG KTGTSSDMKN 

       610        620        630        640        650        660 
LWFCGLTPYY SAAVWIGNDN SSTVDGVYSS TAARLWGDIM KEFHVNLPYK QVQKPASVVT 

       670        680        690        700        710        720 
ANVDRISGKL PTQLSYRDPR GSTVYNEFFI NGTIPTEYDD IHVEAQINKL TGKLASKFTP 

       730        740        750        760        770        780 
SFLVESRVFL RRDYSPGVEL LDQQWLLPYS IDEGGSLPPT EEKNNSNTRD KNKDKNKNKN 

       790        800        810        820        830 
KDKNPSQDKP NNNNNDNNSN NNNNNNDNNN NTKPPENDSN QNHEDNKNKQ

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References

[1]"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes."
Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. expand/collapse author list , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J.
Genome Res. 17:1082-1092(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
[2]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000727 Genomic DNA. Translation: ABS36996.1.
AM412317 Genomic DNA. Translation: CAL84646.1.
RefSeqYP_001255575.1. NC_009495.1.
YP_001388812.1. NC_009698.1.

3D structure databases

ProteinModelPortalA5I6G4.
ModBaseSearch...

Protein-protein interaction databases

STRING413999.CBO3083.

Protein family/group databases

CAZyGT51. Glycosyltransferase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS36996; ABS36996; CLC_2985.
GeneID5185759.
5401037.
KEGGcbh:CLC_2985.
cbo:CBO3083.
PATRIC19368243. VBICloBot22612_3061.

Phylogenomic databases

eggNOGCOG0744.
HOGENOMHOG000041137.
KOK05366.
OMAQNTINDA.
ProtClustDBCLSK883109.

Enzyme and pathway databases

BioCycCBOT413999:GJ72-3183-MONOMER.
CBOT441771:GIWX-2948-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56601. PBP_transp_fold. 1 hit.
TIGRFAMsTIGR02074. PBP_1a_fam. 1 hit.
ProtoNetSearch...

Entry information

Entry namePBPA_CLOBH
AccessionPrimary (citable) accession number: A5I6G4
Secondary accession number(s): A7G7P7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 26, 2007
Last modified: May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents