Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A5I6G4

- PBPA_CLOBH

UniProt

A5I6G4 - PBPA_CLOBH

Protein

Penicillin-binding protein 1A

Gene

pbpA

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei398 – 3981Acyl-ester intermediateBy similarity

    GO - Molecular functioni

    1. penicillin binding Source: InterPro
    2. peptidase activity Source: InterPro
    3. transferase activity, transferring pentosyl groups Source: InterPro

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
    2. regulation of cell shape Source: UniProtKB-KW
    3. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Hydrolase, Transferase

    Keywords - Biological processi

    Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    BioCyciCBOT413999:GJ72-3183-MONOMER.
    CBOT441771:GIWX-2948-MONOMER.
    UniPathwayiUPA00219.

    Protein family/group databases

    CAZyiGT51. Glycosyltransferase Family 51.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 1A
    Short name:
    PBP1a
    Including the following 2 domains:
    Penicillin-insensitive transglycosylase (EC:2.4.2.-)
    Alternative name(s):
    Peptidoglycan TGase
    Penicillin-sensitive transpeptidase (EC:3.4.-.-)
    Alternative name(s):
    DD-transpeptidase
    Gene namesi
    Name:pbpA
    Ordered Locus Names:CBO3083, CLC_2985
    OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
    Taxonomic identifieri441771 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 830830Penicillin-binding protein 1APRO_5000246800Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi413999.CBO3083.

    Structurei

    3D structure databases

    ProteinModelPortaliA5I6G4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini40 – 830791ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni57 – 229173TransglycosylaseBy similarityAdd
    BLAST
    Regioni357 – 641285TranspeptidaseBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi3 – 97Poly-Lys
    Compositional biasi764 – 82865Asn-richAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
    In the C-terminal section; belongs to the transpeptidase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0744.
    HOGENOMiHOG000041137.
    KOiK05366.
    OMAiFLSHQRT.
    OrthoDBiEOG6KDKQS.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR001264. Glyco_trans_51.
    IPR023346. Lysozyme-like_dom.
    IPR011816. PBP_1a.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF00912. Transgly. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.
    SSF56601. SSF56601. 2 hits.
    TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5I6G4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKKKKKRKS SAFKIILNVF LSIFLVAGVA FGGIVFAMIK TAPPLNVQQV    50
    LTFDEPSILY DDKGQYMDKV ITNEQRIVVD YKNVPQNLKN AFVSIEDERF 100
    YKHHGVDIKR FTGVILINVT NKIKRSSKLQ GASTLTQQLI KNTVLSSEVS 150
    IKRKVQEMYL SIQLEKELSK DEILGAYMNS IFLGGNALGV EAASKQYFNK 200
    SVKDLSLIEC AFIAGVPQSP SVYYPYSSAS KKNPSIYLNR TKTVLYKMLD 250
    NGYITQNDYN KALKDLDSKK LVFAKPSAPS NKLAYEWFSI PAIEQVKKDL 300
    KTQYKYDDKQ IHNLLVNGGL KVYTTMNKNL QDKTQNTINN AYYLNSYKSN 350
    GMIYPQASAV IMDYHNGEVK TIIGGRGDQP ARSYNRAASY NYLRPAGSSI 400
    KPLTVYSAAI DSKKATAATG FEDSPIPNNI GRKYSSGAPY NPKNSPDIYY 450
    GYVNVREALM RSINVVAVKL VDKIGLNTSI QYAEKFGIPI DQHDRSSIAS 500
    LSLGELHKGT NPLIMAQAYG VFGNNGTYTE AKLYTKVVDR TGKVLLEPKT 550
    NTKKVLSPEA AFITYDMLQG PVSESGTGPQ ANFGNMEVRG KTGTSSDMKN 600
    LWFCGLTPYY SAAVWIGNDN SSTVDGVYSS TAARLWGDIM KEFHVNLPYK 650
    QVQKPASVVT ANVDRISGKL PTQLSYRDPR GSTVYNEFFI NGTIPTEYDD 700
    IHVEAQINKL TGKLASKFTP SFLVESRVFL RRDYSPGVEL LDQQWLLPYS 750
    IDEGGSLPPT EEKNNSNTRD KNKDKNKNKN KDKNPSQDKP NNNNNDNNSN 800
    NNNNNNDNNN NTKPPENDSN QNHEDNKNKQ 830
    Length:830
    Mass (Da):92,704
    Last modified:June 26, 2007 - v1
    Checksum:iDF9752C616B6D2C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000727 Genomic DNA. Translation: ABS36996.1.
    AM412317 Genomic DNA. Translation: CAL84646.1.
    RefSeqiWP_012048098.1. NC_009698.1.
    YP_001255575.1. NC_009495.1.
    YP_001388812.1. NC_009698.1.

    Genome annotation databases

    EnsemblBacteriaiABS36996; ABS36996; CLC_2985.
    GeneIDi5185759.
    5401037.
    KEGGicbh:CLC_2985.
    cbo:CBO3083.
    PATRICi19368243. VBICloBot22612_3061.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000727 Genomic DNA. Translation: ABS36996.1 .
    AM412317 Genomic DNA. Translation: CAL84646.1 .
    RefSeqi WP_012048098.1. NC_009698.1.
    YP_001255575.1. NC_009495.1.
    YP_001388812.1. NC_009698.1.

    3D structure databases

    ProteinModelPortali A5I6G4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 413999.CBO3083.

    Protein family/group databases

    CAZyi GT51. Glycosyltransferase Family 51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS36996 ; ABS36996 ; CLC_2985 .
    GeneIDi 5185759.
    5401037.
    KEGGi cbh:CLC_2985.
    cbo:CBO3083.
    PATRICi 19368243. VBICloBot22612_3061.

    Phylogenomic databases

    eggNOGi COG0744.
    HOGENOMi HOG000041137.
    KOi K05366.
    OMAi FLSHQRT.
    OrthoDBi EOG6KDKQS.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci CBOT413999:GJ72-3183-MONOMER.
    CBOT441771:GIWX-2948-MONOMER.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR001264. Glyco_trans_51.
    IPR023346. Lysozyme-like_dom.
    IPR011816. PBP_1a.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF00912. Transgly. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    SSF56601. SSF56601. 2 hits.
    TIGRFAMsi TIGR02074. PBP_1a_fam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
    2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
      Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
      PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

    Entry informationi

    Entry nameiPBPA_CLOBH
    AccessioniPrimary (citable) accession number: A5I6G4
    Secondary accession number(s): A7G7P7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3