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A5I6G4

- PBPA_CLOBH

UniProt

A5I6G4 - PBPA_CLOBH

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Protein

Penicillin-binding protein 1A

Gene

pbpA

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei398 – 3981Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro
  2. peptidase activity Source: InterPro
  3. transferase activity, transferring pentosyl groups Source: InterPro

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  3. regulation of cell shape Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-3183-MONOMER.
CBOT441771:GIWX-2948-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:pbpA
Ordered Locus Names:CBO3083, CLC_2985
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Penicillin-binding protein 1APRO_5000246800Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO3083.

Structurei

3D structure databases

ProteinModelPortaliA5I6G4.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini40 – 830791ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 229173TransglycosylaseBy similarityAdd
BLAST
Regioni357 – 641285TranspeptidaseBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 97Poly-Lys
Compositional biasi764 – 82865Asn-richAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0744.
HOGENOMiHOG000041137.
KOiK05366.
OMAiFLSHQRT.
OrthoDBiEOG6KDKQS.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02074. PBP_1a_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

A5I6G4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKKKKKRKS SAFKIILNVF LSIFLVAGVA FGGIVFAMIK TAPPLNVQQV
60 70 80 90 100
LTFDEPSILY DDKGQYMDKV ITNEQRIVVD YKNVPQNLKN AFVSIEDERF
110 120 130 140 150
YKHHGVDIKR FTGVILINVT NKIKRSSKLQ GASTLTQQLI KNTVLSSEVS
160 170 180 190 200
IKRKVQEMYL SIQLEKELSK DEILGAYMNS IFLGGNALGV EAASKQYFNK
210 220 230 240 250
SVKDLSLIEC AFIAGVPQSP SVYYPYSSAS KKNPSIYLNR TKTVLYKMLD
260 270 280 290 300
NGYITQNDYN KALKDLDSKK LVFAKPSAPS NKLAYEWFSI PAIEQVKKDL
310 320 330 340 350
KTQYKYDDKQ IHNLLVNGGL KVYTTMNKNL QDKTQNTINN AYYLNSYKSN
360 370 380 390 400
GMIYPQASAV IMDYHNGEVK TIIGGRGDQP ARSYNRAASY NYLRPAGSSI
410 420 430 440 450
KPLTVYSAAI DSKKATAATG FEDSPIPNNI GRKYSSGAPY NPKNSPDIYY
460 470 480 490 500
GYVNVREALM RSINVVAVKL VDKIGLNTSI QYAEKFGIPI DQHDRSSIAS
510 520 530 540 550
LSLGELHKGT NPLIMAQAYG VFGNNGTYTE AKLYTKVVDR TGKVLLEPKT
560 570 580 590 600
NTKKVLSPEA AFITYDMLQG PVSESGTGPQ ANFGNMEVRG KTGTSSDMKN
610 620 630 640 650
LWFCGLTPYY SAAVWIGNDN SSTVDGVYSS TAARLWGDIM KEFHVNLPYK
660 670 680 690 700
QVQKPASVVT ANVDRISGKL PTQLSYRDPR GSTVYNEFFI NGTIPTEYDD
710 720 730 740 750
IHVEAQINKL TGKLASKFTP SFLVESRVFL RRDYSPGVEL LDQQWLLPYS
760 770 780 790 800
IDEGGSLPPT EEKNNSNTRD KNKDKNKNKN KDKNPSQDKP NNNNNDNNSN
810 820 830
NNNNNNDNNN NTKPPENDSN QNHEDNKNKQ
Length:830
Mass (Da):92,704
Last modified:June 26, 2007 - v1
Checksum:iDF9752C616B6D2C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS36996.1.
AM412317 Genomic DNA. Translation: CAL84646.1.
RefSeqiYP_001255575.1. NC_009495.1.
YP_001388812.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36996; ABS36996; CLC_2985.
GeneIDi5185759.
5401037.
KEGGicbh:CLC_2985.
cbo:CBO3083.
PATRICi19368243. VBICloBot22612_3061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS36996.1 .
AM412317 Genomic DNA. Translation: CAL84646.1 .
RefSeqi YP_001255575.1. NC_009495.1.
YP_001388812.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5I6G4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 413999.CBO3083.

Protein family/group databases

CAZyi GT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS36996 ; ABS36996 ; CLC_2985 .
GeneIDi 5185759.
5401037.
KEGGi cbh:CLC_2985.
cbo:CBO3083.
PATRICi 19368243. VBICloBot22612_3061.

Phylogenomic databases

eggNOGi COG0744.
HOGENOMi HOG000041137.
KOi K05366.
OMAi FLSHQRT.
OrthoDBi EOG6KDKQS.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci CBOT413999:GJ72-3183-MONOMER.
CBOT441771:GIWX-2948-MONOMER.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsi TIGR02074. PBP_1a_fam. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiPBPA_CLOBH
AccessioniPrimary (citable) accession number: A5I6G4
Secondary accession number(s): A7G7P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 26, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3