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A5I5A4

- ASSY_CLOBH

UniProt

A5I5A4 - ASSY_CLOBH

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Protein
Argininosuccinate synthase
Gene
argG, CBO2670, CLC_2544
Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861Citrulline By similarity
Binding sitei91 – 911Citrulline By similarity
Binding sitei116 – 1161ATP; via amide nitrogen By similarity
Binding sitei118 – 1181Aspartate By similarity
Binding sitei122 – 1221Aspartate By similarity
Binding sitei122 – 1221Citrulline By similarity
Binding sitei123 – 1231Aspartate By similarity
Binding sitei126 – 1261Citrulline By similarity
Binding sitei175 – 1751Citrulline By similarity
Binding sitei184 – 1841Citrulline By similarity
Binding sitei260 – 2601Citrulline By similarity
Binding sitei272 – 2721Citrulline By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. argininosuccinate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-2755-MONOMER.
CBOT441771:GIWX-2508-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:CBO2670, CLC_2544
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Argininosuccinate synthaseUniRule annotation
PRO_0000321307Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi413999.CBO2670.

Structurei

3D structure databases

ProteinModelPortaliA5I5A4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiAPPEEAY.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5I5A4-1 [UniParc]FASTAAdd to Basket

« Hide

MKEKVVLAYS GGLDTSIIIP WLKENYDLDV IAVCVNVGQG DDMDYVKTKA    50
IKSGASKIYV EDVKEEFVVD YLYKAIKSEA LYEQDYMLGT SFARPLMAKK 100
LVEIAHKEQA KYICHGCTGK GNDQVRFEVG VKAQDPTIKI IAPWRIWDIK 150
SREDAIDYAK KVGVEVPVTK KKIYSVDKNL WHVSHEGGDL EDLKNEHKED 200
MYFMVTPPEK AKDEPTYLEI YFEKGAPVKI NGEVLNPVDI IDKLNTIGGE 250
NGIGIADIIE NRLVGMKSRG IYETPAGTLL YAAHKKLESV TLDKYTYQYK 300
KIVSAQYGEL VYNGLWFTSL REAIDAFVDK TQENVTGTVQ LKLYKGNIKP 350
CSVDTEYALY DEGISSFGES ELYSHKDAEG FINLFGLPSK IKALKNF 397
Length:397
Mass (Da):44,731
Last modified:June 26, 2007 - v1
Checksum:i762341059F7DF962
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS37966.1.
AM412317 Genomic DNA. Translation: CAL84231.1.
RefSeqiWP_011986966.1. NC_009698.1.
YP_001255169.1. NC_009495.1.
YP_001388382.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS37966; ABS37966; CLC_2544.
GeneIDi5186925.
5398862.
KEGGicbh:CLC_2544.
cbo:CBO2670.
PATRICi19367421. VBICloBot22612_2653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS37966.1 .
AM412317 Genomic DNA. Translation: CAL84231.1 .
RefSeqi WP_011986966.1. NC_009698.1.
YP_001255169.1. NC_009495.1.
YP_001388382.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5I5A4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 413999.CBO2670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS37966 ; ABS37966 ; CLC_2544 .
GeneIDi 5186925.
5398862.
KEGGi cbh:CLC_2544.
cbo:CBO2670.
PATRICi 19367421. VBICloBot22612_2653.

Phylogenomic databases

eggNOGi COG0137.
HOGENOMi HOG000230093.
KOi K01940.
OMAi APPEEAY.
OrthoDBi EOG6K9QCV.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
BioCyci CBOT413999:GJ72-2755-MONOMER.
CBOT441771:GIWX-2508-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiASSY_CLOBH
AccessioniPrimary (citable) accession number: A5I5A4
Secondary accession number(s): A7G6G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 26, 2007
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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