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A5I5A4

- ASSY_CLOBH

UniProt

A5I5A4 - ASSY_CLOBH

Protein

Argininosuccinate synthase

Gene

argG

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861CitrullineUniRule annotation
    Binding sitei91 – 911CitrullineUniRule annotation
    Binding sitei116 – 1161ATP; via amide nitrogenUniRule annotation
    Binding sitei118 – 1181AspartateUniRule annotation
    Binding sitei122 – 1221AspartateUniRule annotation
    Binding sitei122 – 1221CitrullineUniRule annotation
    Binding sitei123 – 1231AspartateUniRule annotation
    Binding sitei126 – 1261CitrullineUniRule annotation
    Binding sitei175 – 1751CitrullineUniRule annotation
    Binding sitei184 – 1841CitrullineUniRule annotation
    Binding sitei260 – 2601CitrullineUniRule annotation
    Binding sitei272 – 2721CitrullineUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 169ATPUniRule annotation

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBOT413999:GJ72-2755-MONOMER.
    CBOT441771:GIWX-2508-MONOMER.
    UniPathwayiUPA00068; UER00113.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
    Alternative name(s):
    Citrulline--aspartate ligaseUniRule annotation
    Gene namesi
    Name:argGUniRule annotation
    Ordered Locus Names:CBO2670, CLC_2544
    OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
    Taxonomic identifieri441771 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397Argininosuccinate synthasePRO_0000321307Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi413999.CBO2670.

    Structurei

    3D structure databases

    ProteinModelPortaliA5I5A4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0137.
    HOGENOMiHOG000230093.
    KOiK01940.
    OMAiAPPEEAY.
    OrthoDBiEOG6K9QCV.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5I5A4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKEKVVLAYS GGLDTSIIIP WLKENYDLDV IAVCVNVGQG DDMDYVKTKA    50
    IKSGASKIYV EDVKEEFVVD YLYKAIKSEA LYEQDYMLGT SFARPLMAKK 100
    LVEIAHKEQA KYICHGCTGK GNDQVRFEVG VKAQDPTIKI IAPWRIWDIK 150
    SREDAIDYAK KVGVEVPVTK KKIYSVDKNL WHVSHEGGDL EDLKNEHKED 200
    MYFMVTPPEK AKDEPTYLEI YFEKGAPVKI NGEVLNPVDI IDKLNTIGGE 250
    NGIGIADIIE NRLVGMKSRG IYETPAGTLL YAAHKKLESV TLDKYTYQYK 300
    KIVSAQYGEL VYNGLWFTSL REAIDAFVDK TQENVTGTVQ LKLYKGNIKP 350
    CSVDTEYALY DEGISSFGES ELYSHKDAEG FINLFGLPSK IKALKNF 397
    Length:397
    Mass (Da):44,731
    Last modified:June 26, 2007 - v1
    Checksum:i762341059F7DF962
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000727 Genomic DNA. Translation: ABS37966.1.
    AM412317 Genomic DNA. Translation: CAL84231.1.
    RefSeqiWP_011986966.1. NC_009698.1.
    YP_001255169.1. NC_009495.1.
    YP_001388382.1. NC_009698.1.

    Genome annotation databases

    EnsemblBacteriaiABS37966; ABS37966; CLC_2544.
    GeneIDi5186925.
    5398862.
    KEGGicbh:CLC_2544.
    cbo:CBO2670.
    PATRICi19367421. VBICloBot22612_2653.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000727 Genomic DNA. Translation: ABS37966.1 .
    AM412317 Genomic DNA. Translation: CAL84231.1 .
    RefSeqi WP_011986966.1. NC_009698.1.
    YP_001255169.1. NC_009495.1.
    YP_001388382.1. NC_009698.1.

    3D structure databases

    ProteinModelPortali A5I5A4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 413999.CBO2670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS37966 ; ABS37966 ; CLC_2544 .
    GeneIDi 5186925.
    5398862.
    KEGGi cbh:CLC_2544.
    cbo:CBO2670.
    PATRICi 19367421. VBICloBot22612_2653.

    Phylogenomic databases

    eggNOGi COG0137.
    HOGENOMi HOG000230093.
    KOi K01940.
    OMAi APPEEAY.
    OrthoDBi EOG6K9QCV.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .
    BioCyci CBOT413999:GJ72-2755-MONOMER.
    CBOT441771:GIWX-2508-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
    2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
      Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
      PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

    Entry informationi

    Entry nameiASSY_CLOBH
    AccessioniPrimary (citable) accession number: A5I5A4
    Secondary accession number(s): A7G6G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3