A5I4X1 (AROE_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	CBO2540, CLC_2398

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021275
Regions
	Nucleotide binding	125 – 129	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A5I4X1 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: 5BB7CDDE73F6FCF6	FASTA	258	29,691
	10 20 30 40 50 60 MYTTGLIGKN INYSESPEIH NNYYKKNNIP FFYKIFNLKQ DQIDDFIKNL HKNNIKGFNV 70 80 90 100 110 120 TIPYKETILQ YLNDIVYPAD KIGAVNTVAV QEDKLIGYNT DYIGFIKSLQ YYNIQVKNFK 130 140 150 160 170 180 CLIIGSGGSA KCIYYALKEL NARDICIVSR NPEKARLKFE KKVKILNIKD ENKLDRYDLI 190 200 210 220 230 240 VNCTPIGGPN LKEQKPIELK EIKKNCVVYD LNYTPKRSKL LKEAKENGAF IINGEKMLIF 250 QAYSAIGLWC LNGIKGGR

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS37187.1. AM412317 Genomic DNA. Translation: CAL84094.1.
RefSeq	YP_001255038.1. NC_009495.1. YP_001388241.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5I4X1.
ModBase	Search...
Protein-protein interaction databases
STRING	A5I4X1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5187986. 5399448.
GenomeReviews	Gene locus CBO2540 in contig AM412317_GR. Gene locus CLC_2398 in contig CP000727_GR.
KEGG	cbh:CLC_2398. cbo:CBO2540.
PATRIC	19367155. VBICloBot22612_2520.
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	TLFMKKG.
ProtClustDB	CLSK912105.
Enzyme and pathway databases
BioCyc	CBOT36826:CBO2540-MONOMER. CBOT441771:CLC_2398-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_CLOBH

Accession

Primary (citable) accession number: A5I4X1
Secondary accession number(s): A7G626

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 26, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program