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A5I4I7

- PNP_CLOBH

UniProt

A5I4I7 - PNP_CLOBH

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, CBO2413, CLC_2259
Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-2482-MONOMER.
CBOT441771:GIWX-2225-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:CBO2413, CLC_2259
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329596Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO2413.

Structurei

3D structure databases

ProteinModelPortaliA5I4I7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61261KHAdd
BLAST
Domaini622 – 69069S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5I4I7-1 [UniParc]FASTAAdd to Basket

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MIHTLETTVA GRKMKVDFGK TGMLSNAAIF MSYGDTVVMI NANASKEPRE    50
GIDFFPLSVE YEERLYSVGK IPGGFIKREG KPSDKSILHA RSIDRPLRPL 100
FPKGYRNDVQ IVNTVLSVEQ DNLPEILAIN GSSLALCLSS IPFTTPVAAV 150
SVGLVDGEFI INPTVAQREN TILDLTVCAT KERVMMVEAG GQEIDEETMY 200
SAIMFGFEEC KNIVAFQEEA VAKFGKTKNE PVLYKADEEV EKEVKSFAFD 250
MIKEAMYIMD KDERNAQLDK VKEKISEEFS EKYEDKVADI AEVIYKTQKE 300
IVRNMLLNED RRPDGRAFDE VRPISCEVGI LPRTHGTGLF TRGLTQVMTV 350
ATLGALGDVQ ILDGIAEEES KRYMHHYNFP SYSVGEVRPL RGPGRREIGH 400
GALAERALEP LIPSQSEFPY TIRLVSEVLS SNGSTSQASV CGSTLALLDA 450
GVPIKRPAAG IAMGLITSED LEKEKVITDI QGIEDFFGDM DFKVAGTEKG 500
ITSIQFDTKI KGLSNSCVKD ALEGAKKARL HILGKIKECI PEPRKELSKY 550
APRTEIICID PEKIRDVIGA GGKVINKIIA DTNVKIEIKE DGKIFVTSNN 600
EPEGVKKAIS IIEGLTKEVV QGEIYLGKVT KTTNFGAFVE ILPGKEGLVH 650
ISKLDFARVE KVEDVVSVGD EILVKVTDID NQGRINLSRK DAIAKKEEEK 700
DK 702
Length:702
Mass (Da):77,454
Last modified:June 26, 2007 - v1
Checksum:i70A40D92A1091252
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS36368.1.
AM412317 Genomic DNA. Translation: CAL83959.1.
RefSeqiYP_001254908.1. NC_009495.1.
YP_001388103.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36368; ABS36368; CLC_2259.
GeneIDi5186668.
5400150.
KEGGicbh:CLC_2259.
cbo:CBO2413.
PATRICi19366893. VBICloBot22612_2389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS36368.1 .
AM412317 Genomic DNA. Translation: CAL83959.1 .
RefSeqi YP_001254908.1. NC_009495.1.
YP_001388103.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5I4I7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 413999.CBO2413.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS36368 ; ABS36368 ; CLC_2259 .
GeneIDi 5186668.
5400150.
KEGGi cbh:CLC_2259.
cbo:CBO2413.
PATRICi 19366893. VBICloBot22612_2389.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CBOT413999:GJ72-2482-MONOMER.
CBOT441771:GIWX-2225-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiPNP_CLOBH
AccessioniPrimary (citable) accession number: A5I4I7
Secondary accession number(s): A7G5N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 26, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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