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A5I427

- BIOB_CLOBH

UniProt

A5I427 - BIOB_CLOBH

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Protein
Biotin synthase
Gene
bioB, CBO2259, CLC_2183
Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi268 – 2681Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-2322-MONOMER.
CBOT441771:GIWX-2150-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:CBO2259, CLC_2183
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Biotin synthaseUniRule annotation
PRO_0000381313Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi413999.CBO2259.

Structurei

3D structure databases

ProteinModelPortaliA5I427.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5I427-1 [UniParc]FASTAAdd to Basket

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MSNIIKYKKK ILNGDLLTKE EVEELLEEDI TDLAATANEI RESLCGNKFD    50
LCTIINGKSG RCQENCKYCA QSAHFDTDII EYNILNSDRI INSAISNYNK 100
GVHRFSVVTS GRALNNNEVD TLCKTYSKLK ETCSIRLCAS HGLLKYEDLK 150
RLKDSGVTRY HNNLETSRKF FTKICTTHKY DDKIETIKNA KKAGIEICSG 200
GIIGLGETME DRIDMAFTLR ELSVESVPVN ILNPIKGTPL ENQEILSYEE 250
IIKTLALFRF ILPTVQIRLA GGRTIISDKG KKALESGVNG AISGDMLTTL 300
GIETSEDIKM IKNLGFEV 318
Length:318
Mass (Da):35,506
Last modified:June 26, 2007 - v1
Checksum:i04A5868F15F62E4C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS35996.1.
AM412317 Genomic DNA. Translation: CAL83798.1.
RefSeqiYP_001254750.1. NC_009495.1.
YP_001388029.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS35996; ABS35996; CLC_2183.
GeneIDi5186514.
5401796.
KEGGicbh:CLC_2183.
cbo:CBO2259.
PATRICi19366573. VBICloBot22612_2229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000727 Genomic DNA. Translation: ABS35996.1 .
AM412317 Genomic DNA. Translation: CAL83798.1 .
RefSeqi YP_001254750.1. NC_009495.1.
YP_001388029.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5I427.
ModBasei Search...

Protein-protein interaction databases

STRINGi 413999.CBO2259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS35996 ; ABS35996 ; CLC_2183 .
GeneIDi 5186514.
5401796.
KEGGi cbh:CLC_2183.
cbo:CBO2259.
PATRICi 19366573. VBICloBot22612_2229.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CBOT413999:GJ72-2322-MONOMER.
CBOT441771:GIWX-2150-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiBIOB_CLOBH
AccessioniPrimary (citable) accession number: A5I427
Secondary accession number(s): A7G5G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 26, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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