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A5I421

- THIM2_CLOBH

UniProt

A5I421 - THIM2_CLOBH

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Protein

Hydroxyethylthiazole kinase 2

Gene

thiM2

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391Substrate; via amide nitrogenUniRule annotation
Binding sitei115 – 1151ATPUniRule annotation
Binding sitei168 – 1681ATPUniRule annotation
Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-2316-MONOMER.
CBOT441771:GIWX-2143-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 2UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 2UniRule annotation
Short name:
TH kinase 2UniRule annotation
Short name:
Thz kinase 2UniRule annotation
Gene namesi
Name:thiM2UniRule annotation
Ordered Locus Names:CBO2253, CLC_2176
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Hydroxyethylthiazole kinase 2PRO_0000383843Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO2253.

Structurei

3D structure databases

ProteinModelPortaliA5I421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

A5I421-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQIRQSIKLK KPLIHYITNP ISINDCANII LAAGAKPIMA EHPLEVSEIT
60 70 80 90 100
SVSKSLGVNL GNITDNKMKS MLISGKTAYE NKIPQVIDLV GVGCSKLRLD
110 120 130 140 150
YAKKFISECH PNVIKGNMSE IKAIYGIKSS AKGIDVGACD IITKQNFDEN
160 170 180 190 200
IEMIKRLSME TGSVVAATGV VDIISNGTYT YIISNGCEML SMITGTGCML
210 220 230 240 250
TGLIASYISS ENILDGTVLA VALMGICGEL SQHAKGTGSF RNELTDNMFS
260
ISDDIIIKKI RINSY
Length:265
Mass (Da):28,576
Last modified:September 22, 2009 - v2
Checksum:iF2F92C9C43F88AE8
GO

Sequence cautioni

The sequence CAL83792.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM412317 Genomic DNA. Translation: CAL83792.1. Different initiation.
CP000727 Genomic DNA. Translation: ABS36898.1.
RefSeqiYP_001254744.1. NC_009495.1.
YP_001388022.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36898; ABS36898; CLC_2176.
GeneIDi5186508.
5400907.
KEGGicbh:CLC_2176.
cbo:CBO2253.
PATRICi19366559. VBICloBot22612_2222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM412317 Genomic DNA. Translation: CAL83792.1 . Different initiation.
CP000727 Genomic DNA. Translation: ABS36898.1 .
RefSeqi YP_001254744.1. NC_009495.1.
YP_001388022.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5I421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 413999.CBO2253.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS36898 ; ABS36898 ; CLC_2176 .
GeneIDi 5186508.
5400907.
KEGGi cbh:CLC_2176.
cbo:CBO2253.
PATRICi 19366559. VBICloBot22612_2222.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
KOi K00878.
OrthoDBi EOG628F8M.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci CBOT413999:GJ72-2316-MONOMER.
CBOT441771:GIWX-2143-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiTHIM2_CLOBH
AccessioniPrimary (citable) accession number: A5I421
Secondary accession number(s): A7G5F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: October 29, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3