Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A5I421 (THIM2_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase 2

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 2
Short name=TH kinase 2
Short name=Thz kinase 2
Gene names
Name:thiM2
Ordered Locus Names:CBO2253, CLC_2176
OrganismClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Reference proteome] [HAMAP]
Taxonomic identifier441771 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence caution

The sequence CAL83792.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Hydroxyethylthiazole kinase 2 HAMAP-Rule MF_00228
PRO_0000383843

Sites

Binding site391Substrate; via amide nitrogen By similarity
Binding site1151ATP By similarity
Binding site1681ATP By similarity
Binding site1951Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5I421 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: F2F92C9C43F88AE8

FASTA26528,576
        10         20         30         40         50         60 
MQIRQSIKLK KPLIHYITNP ISINDCANII LAAGAKPIMA EHPLEVSEIT SVSKSLGVNL 

        70         80         90        100        110        120 
GNITDNKMKS MLISGKTAYE NKIPQVIDLV GVGCSKLRLD YAKKFISECH PNVIKGNMSE 

       130        140        150        160        170        180 
IKAIYGIKSS AKGIDVGACD IITKQNFDEN IEMIKRLSME TGSVVAATGV VDIISNGTYT 

       190        200        210        220        230        240 
YIISNGCEML SMITGTGCML TGLIASYISS ENILDGTVLA VALMGICGEL SQHAKGTGSF 

       250        260 
RNELTDNMFS ISDDIIIKKI RINSY 

« Hide

References

[1]"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes."
Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. expand/collapse author list , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J.
Genome Res. 17:1082-1092(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
[2]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM412317 Genomic DNA. Translation: CAL83792.1. Different initiation.
CP000727 Genomic DNA. Translation: ABS36898.1.
RefSeqYP_001254744.1. NC_009495.1.
YP_001388022.1. NC_009698.1.

3D structure databases

ProteinModelPortalA5I421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING413999.CBO2253.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS36898; ABS36898; CLC_2176.
GeneID5186508.
5400907.
KEGGcbh:CLC_2176.
cbo:CBO2253.
PATRIC19366559. VBICloBot22612_2222.

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OrthoDBEOG628F8M.
ProtClustDBCLSK972179.

Enzyme and pathway databases

BioCycCBOT413999:GJ72-2316-MONOMER.
CBOT441771:GIWX-2143-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
ProtoNetSearch...

Entry information

Entry nameTHIM2_CLOBH
AccessionPrimary (citable) accession number: A5I421
Secondary accession number(s): A7G5F7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: February 19, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways