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A5I421

- THIM2_CLOBH

UniProt

A5I421 - THIM2_CLOBH

Protein

Hydroxyethylthiazole kinase 2

Gene

thiM2

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391Substrate; via amide nitrogenUniRule annotation
    Binding sitei115 – 1151ATPUniRule annotation
    Binding sitei168 – 1681ATPUniRule annotation
    Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBOT413999:GJ72-2316-MONOMER.
    CBOT441771:GIWX-2143-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinase 2UniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase 2UniRule annotation
    Short name:
    TH kinase 2UniRule annotation
    Short name:
    Thz kinase 2UniRule annotation
    Gene namesi
    Name:thiM2UniRule annotation
    Ordered Locus Names:CBO2253, CLC_2176
    OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
    Taxonomic identifieri441771 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 265265Hydroxyethylthiazole kinase 2PRO_0000383843Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi413999.CBO2253.

    Structurei

    3D structure databases

    ProteinModelPortaliA5I421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    HOGENOMiHOG000114352.
    KOiK00878.
    OrthoDBiEOG628F8M.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5I421-1 [UniParc]FASTAAdd to Basket

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    MQIRQSIKLK KPLIHYITNP ISINDCANII LAAGAKPIMA EHPLEVSEIT    50
    SVSKSLGVNL GNITDNKMKS MLISGKTAYE NKIPQVIDLV GVGCSKLRLD 100
    YAKKFISECH PNVIKGNMSE IKAIYGIKSS AKGIDVGACD IITKQNFDEN 150
    IEMIKRLSME TGSVVAATGV VDIISNGTYT YIISNGCEML SMITGTGCML 200
    TGLIASYISS ENILDGTVLA VALMGICGEL SQHAKGTGSF RNELTDNMFS 250
    ISDDIIIKKI RINSY 265
    Length:265
    Mass (Da):28,576
    Last modified:September 22, 2009 - v2
    Checksum:iF2F92C9C43F88AE8
    GO

    Sequence cautioni

    The sequence CAL83792.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM412317 Genomic DNA. Translation: CAL83792.1. Different initiation.
    CP000727 Genomic DNA. Translation: ABS36898.1.
    RefSeqiWP_011986722.1. NC_009698.1.
    YP_001254744.1. NC_009495.1.
    YP_001388022.1. NC_009698.1.

    Genome annotation databases

    EnsemblBacteriaiABS36898; ABS36898; CLC_2176.
    GeneIDi5186508.
    5400907.
    KEGGicbh:CLC_2176.
    cbo:CBO2253.
    PATRICi19366559. VBICloBot22612_2222.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM412317 Genomic DNA. Translation: CAL83792.1 . Different initiation.
    CP000727 Genomic DNA. Translation: ABS36898.1 .
    RefSeqi WP_011986722.1. NC_009698.1.
    YP_001254744.1. NC_009495.1.
    YP_001388022.1. NC_009698.1.

    3D structure databases

    ProteinModelPortali A5I421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 413999.CBO2253.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS36898 ; ABS36898 ; CLC_2176 .
    GeneIDi 5186508.
    5400907.
    KEGGi cbh:CLC_2176.
    cbo:CBO2253.
    PATRICi 19366559. VBICloBot22612_2222.

    Phylogenomic databases

    eggNOGi COG2145.
    HOGENOMi HOG000114352.
    KOi K00878.
    OrthoDBi EOG628F8M.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci CBOT413999:GJ72-2316-MONOMER.
    CBOT441771:GIWX-2143-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
    2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
      Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
      PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

    Entry informationi

    Entry nameiTHIM2_CLOBH
    AccessioniPrimary (citable) accession number: A5I421
    Secondary accession number(s): A7G5F7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 58 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3