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Protein

Hydroxyethylthiazole kinase 2

Gene

thiM2

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase 1 (thiM1), Hydroxyethylthiazole kinase 2 (thiM2)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391Substrate; via amide nitrogenUniRule annotation
Binding sitei115 – 1151ATPUniRule annotation
Binding sitei168 – 1681ATPUniRule annotation
Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-2316-MONOMER.
CBOT441771:GIWX-2143-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 2UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 2UniRule annotation
Short name:
TH kinase 2UniRule annotation
Short name:
Thz kinase 2UniRule annotation
Gene namesi
Name:thiM2UniRule annotation
Ordered Locus Names:CBO2253, CLC_2176
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Hydroxyethylthiazole kinase 2PRO_0000383843Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA5I421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

A5I421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIRQSIKLK KPLIHYITNP ISINDCANII LAAGAKPIMA EHPLEVSEIT
60 70 80 90 100
SVSKSLGVNL GNITDNKMKS MLISGKTAYE NKIPQVIDLV GVGCSKLRLD
110 120 130 140 150
YAKKFISECH PNVIKGNMSE IKAIYGIKSS AKGIDVGACD IITKQNFDEN
160 170 180 190 200
IEMIKRLSME TGSVVAATGV VDIISNGTYT YIISNGCEML SMITGTGCML
210 220 230 240 250
TGLIASYISS ENILDGTVLA VALMGICGEL SQHAKGTGSF RNELTDNMFS
260
ISDDIIIKKI RINSY
Length:265
Mass (Da):28,576
Last modified:September 22, 2009 - v2
Checksum:iF2F92C9C43F88AE8
GO

Sequence cautioni

The sequence CAL83792.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM412317 Genomic DNA. Translation: CAL83792.1. Different initiation.
CP000727 Genomic DNA. Translation: ABS36898.1.
RefSeqiWP_011986722.1. NC_009698.1.
YP_001254744.1. NC_009495.1.
YP_001388022.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36898; ABS36898; CLC_2176.
GeneIDi5186508.
5400907.
KEGGicbh:CLC_2176.
cbo:CBO2253.
PATRICi19366559. VBICloBot22612_2222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM412317 Genomic DNA. Translation: CAL83792.1. Different initiation.
CP000727 Genomic DNA. Translation: ABS36898.1.
RefSeqiWP_011986722.1. NC_009698.1.
YP_001254744.1. NC_009495.1.
YP_001388022.1. NC_009698.1.

3D structure databases

ProteinModelPortaliA5I421.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS36898; ABS36898; CLC_2176.
GeneIDi5186508.
5400907.
KEGGicbh:CLC_2176.
cbo:CBO2253.
PATRICi19366559. VBICloBot22612_2222.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciCBOT413999:GJ72-2316-MONOMER.
CBOT441771:GIWX-2143-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiTHIM2_CLOBH
AccessioniPrimary (citable) accession number: A5I421
Secondary accession number(s): A7G5F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: June 24, 2015
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.