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A5I3X9

- PDXA_CLOBH

UniProt

A5I3X9 - PDXA_CLOBH

Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei141 – 1411SubstrateUniRule annotation
    Binding sitei142 – 1421SubstrateUniRule annotation
    Metal bindingi171 – 1711Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi215 – 2151Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi270 – 2701Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei278 – 2781SubstrateUniRule annotation
    Binding sitei296 – 2961SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, NADP

    Enzyme and pathway databases

    BioCyciCBOT413999:GJ72-2273-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
    Gene namesi
    Name:pdxAUniRule annotation
    Ordered Locus Names:CBO2208
    OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
    Taxonomic identifieri441771 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001986: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3343344-hydroxythreonine-4-phosphate dehydrogenasePRO_1000051498Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi413999.CBO2208.

    Structurei

    3D structure databases

    ProteinModelPortaliA5I3X9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000221593.
    KOiK00097.
    OMAiAIPMGDP.
    OrthoDBiEOG6GN6ZC.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5I3X9-1 [UniParc]FASTAAdd to Basket

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    MINNKPIIGI PIGDPAGVGP EIVVKSLTEA EVYEKCNPIL IGDAKVIKQA    50
    MGFCNVNLNI NSIKKADEGK FTLGTIDLID LNNIDIDELK IGKVQGIAGK 100
    AAFEYIKKSV EMAKEGELDA IATTPINKES LREGNVNYIG HTEILADLTD 150
    TEDPLTMFEV RGMRVFFLTR HVSLRKACDL VTKERVLDYI IRCSEALEKL 200
    GVKDGKMAVA GLNPHSGEHG LFGDEEMKAV VPAIEEAQKM GYKVEGPIGA 250
    DSVFHLALKG RYNSVLSLYH DQGHIATKTL DFERTIAVTN GMPILRTSVD 300
    HGTAFDIAGT GQASSVSMVE AIILAAKYSP KFKK 334
    Length:334
    Mass (Da):36,273
    Last modified:June 26, 2007 - v1
    Checksum:iD48D3A5B50A457F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM412317 Genomic DNA. Translation: CAL83749.1.
    RefSeqiYP_001254702.1. NC_009495.1.

    Genome annotation databases

    GeneIDi5186463.
    KEGGicbo:CBO2208.
    PATRICi19366467. VBICloBot22612_2176.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM412317 Genomic DNA. Translation: CAL83749.1 .
    RefSeqi YP_001254702.1. NC_009495.1.

    3D structure databases

    ProteinModelPortali A5I3X9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 413999.CBO2208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5186463.
    KEGGi cbo:CBO2208.
    PATRICi 19366467. VBICloBot22612_2176.

    Phylogenomic databases

    HOGENOMi HOG000221593.
    KOi K00097.
    OMAi AIPMGDP.
    OrthoDBi EOG6GN6ZC.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci CBOT413999:GJ72-2273-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

    Entry informationi

    Entry nameiPDXA_CLOBH
    AccessioniPrimary (citable) accession number: A5I3X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3