A5I348 (MSRA_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide methionine sulfoxide reductase MsrA
Short name=Protein-methionine-S-oxide reductase
EC=1.8.4.11

Alternative name(s):
Peptide-methionine (S)-S-oxide reductase
Short name=Peptide Met(O) reductase

Gene names

Name:	msrA
Ordered Locus Names:	CBO1923, CLC_1868

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity. HAMAP MF_01401
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01401 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01401
Sequence similarities	Belongs to the MsrA Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Peptide methionine sulfoxide reductase MsrA HAMAP MF_01401		PRO_1000068319
Sites
	Active site	10	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5I348 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: E4458E31DD3FC694	FASTA	157	18,178
	10 20 30 40 50 60 MKEIVLAGGC FWGVEEYMSR IEGIVETKVG YANGIKENPS YEEVCSGVTG HAEACYIKYD 70 80 90 100 110 120 ESIISLEELL NKFWSIIDPT VLNKQGNDRG TQYRTGIFYL DEKDLNVIIK SKYQEQKNYR 130 140 150 KPIVTEVEPL KCFYEAEEYH QKYLKKNPGG YCHIHLD

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS38947.1. AM412317 Genomic DNA. Translation: CAL83465.1.
RefSeq	YP_001254426.1. NC_009495.1. YP_001387723.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5I348.
SMR	A5I348. Positions 1-156.
ModBase	Search...
Protein-protein interaction databases
STRING	A5I348.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5186178. 5399299.
GenomeReviews	Gene locus CBO1923 in contig AM412317_GR. Gene locus CLC_1868 in contig CP000727_GR.
KEGG	cbh:CLC_1868. cbo:CBO1923.
PATRIC	19365905. VBICloBot22612_1895.
Phylogenomic databases
eggNOG	COG0225.
HOGENOM	HBG748152.
OMA	ERKIMTE.
ProtClustDB	PRK14054.
Enzyme and pathway databases
BioCyc	CBOT36826:CBO1923-MONOMER. CBOT441771:CLC_1868-MONOMER.
Family and domain databases
HAMAP	MF_01401. MsrA. [Tree]
InterPro	IPR002569. Peptide_Met_Sox_Rdtase_MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit.
KO	K07304.
Pfam	PF01625. PMSR. 1 hit. [Graphical view]
SUPFAM	SSF55068. MsrA. 1 hit.
TIGRFAMs	TIGR00401. MsrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRA_CLOBH

Accession

Primary (citable) accession number: A5I348
Secondary accession number(s): A7G4K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 26, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program