Reviewed, UniProtKB/Swiss-Prot A5I348 (MSRA_CLOBH)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA
      Short name=Protein-methionine-S-oxide reductase
    EC=1.8.4.11
Alternative name(s):
    Peptide-methionine (S)-S-oxide reductase
      Short name=Peptide Met(O) reductase

Gene names

Name:	msrA
Ordered Locus Names:	CBO1923, CLC_1868

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity.
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (S)-S-oxide + thioredoxin. L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin.
Sequence similarities	Belongs to the msrA Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Peptide methionine sulfoxide reductase msrA		PRO_1000068319
Sites
	Active site	10	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5I348-1 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: E4458E31DD3FC694	FASTA	157	18,178
	10 20 30 40 50 60 MKEIVLAGGC FWGVEEYMSR IEGIVETKVG YANGIKENPS YEEVCSGVTG HAEACYIKYD 70 80 90 100 110 120 ESIISLEELL NKFWSIIDPT VLNKQGNDRG TQYRTGIFYL DEKDLNVIIK SKYQEQKNYR 130 140 150 KPIVTEVEPL KCFYEAEEYH QKYLKKNPGG YCHIHLD

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000727 Genomic DNA. Translation: ABS38947.1. AM412317 Genomic DNA. Translation: CAL83465.1.
RefSeq	YP_001254426.1. YP_001387723.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5186178. 5399299.
GenomeReviews	Gene locus CBO1923 in contig AM412317_GR. Gene locus CLC_1868 in contig CP000727_GR.
KEGG	cbh:CLC_1868. cbo:CBO1923.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01401. [Tree]
InterPro	IPR002569. MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit.
Pfam	PF01625. PMSR. 1 hit. [Graphical view]
ProDom	PD003489. PMSR. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00401. msrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRA_CLOBH

Accession

Primary (citable) accession number: A5I348
Secondary accession number(s): A7G4K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 26, 2007
Last modified:	November 4, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)