A5I323 (AROK_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	CBO1899, CLC_1843

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm By similarity HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW shikimate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Shikimate kinase HAMAP MF_00109		PRO_1000094381
Regions
	Nucleotide binding	11 – 16	6	ATP By similarity
Sites
	Metal binding	15	1	Magnesium By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	79	1	Substrate; via amide nitrogen By similarity
	Binding site	119	1	ATP By similarity
	Binding site	137	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5I323 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: E78BED50CCF984EE	FASTA	170	20,068
	10 20 30 40 50 60 MENIVLIGMP LSGKSTLGRE LSKILKYDLI DTDTLIEEME DKSIKEIFKI YGEDYFREKE 70 80 90 100 110 120 LKIINKLKKE SNKVISTGGG LPIYNKNIYE LKKIGFTVYL KVPLEELIKR MVKKEYDTRP 130 140 150 160 170 LLKNNDTKFL EEMYKNRIEI YEKAHTIICN TNYKESLITI VRAYKKWKGI

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS36174.1. AM412317 Genomic DNA. Translation: CAL83440.1.
RefSeq	YP_001254401.1. NC_009495.1. YP_001387698.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5I323.
ModBase	Search...
Protein-protein interaction databases
STRING	A5I323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5186154. 5401294.
GenomeReviews	Gene locus CBO1899 in contig AM412317_GR. Gene locus CLC_1843 in contig CP000727_GR.
KEGG	cbh:CLC_1843. cbo:CBO1899.
PATRIC	19365857. VBICloBot22612_1871.
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HBG335815.
OMA	MIISERV.
ProtClustDB	CLSK912193.
Enzyme and pathway databases
BioCyc	CBOT36826:CBO1899-MONOMER. CBOT441771:CLC_1843-MONOMER.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shikimate_kinase. [Graphical view]
KO	K00891.
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_CLOBH

Accession

Primary (citable) accession number: A5I323
Secondary accession number(s): A7G4I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 26, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program