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Protein

Alanine racemase

Gene

alr

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Active sitei267 – 2671Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei315 – 3151Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-1715-MONOMER.
CBOT441771:GIWX-1653-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alrImported
Synonyms:alr-2Imported
Ordered Locus Names:CBO1660Imported, CLC_1685Imported
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)Imported
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002409 Componenti: Chromosome UP000001986 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO1660.

Structurei

3D structure databases

ProteinModelPortaliA5I2C8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiNTVMVDV.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5I2C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRNHRPVWE EVNLDNLSYN MRNIKKKVNC KEIFAVVKAN GYGHGALDIA
60 70 80 90 100
STLLENGATR LSVACLSEAT ELREGGITCP INILGITPPT LFEDIIDYNI
110 120 130 140 150
EPVVFSYDCA NQLSKAASLK NRIVKTHIAV DTGMGRLGFD PTVESLEEIK
160 170 180 190 200
RIQDLSNIKI EGLCSHFSSS DEKDKSYSEY QFKIFQNFRK KLIEMNMKIP
210 220 230 240 250
IYHMSNSAAI IDLPSTYLDG VRAGIILYGY YPSMEVNKEN LHIKQVMTLK
260 270 280 290 300
ANIVHIKTID SGKYVGYNRK FKTKRKSKIA ILPLGYGDGY TRFLSNRGKV
310 320 330 340 350
IINGQYAPIV GNICMDQCMI DVTDIKEVKV GDEVIIIGKD NINNLRYDAE
360 370 380
DIASKVGMTS GEIMSIISKR VPRVYIKNGN IVKVRNYIA
Length:389
Mass (Da):43,759
Last modified:June 26, 2007 - v1
Checksum:i832BF5FDCF7C0140
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS36354.1.
AM412317 Genomic DNA. Translation: CAL83195.1.
RefSeqiYP_001254158.1. NC_009495.1.
YP_001387541.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36354; ABS36354; CLC_1685.
GeneIDi5185915.
5400037.
KEGGicbh:CLC_1685.
cbo:CBO1660.
PATRICi19365379. VBICloBot22612_1633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS36354.1.
AM412317 Genomic DNA. Translation: CAL83195.1.
RefSeqiYP_001254158.1. NC_009495.1.
YP_001387541.1. NC_009698.1.

3D structure databases

ProteinModelPortaliA5I2C8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi413999.CBO1660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS36354; ABS36354; CLC_1685.
GeneIDi5185915.
5400037.
KEGGicbh:CLC_1685.
cbo:CBO1660.
PATRICi19365379. VBICloBot22612_1633.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiNTVMVDV.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciCBOT413999:GJ72-1715-MONOMER.
CBOT441771:GIWX-1653-MONOMER.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 3502Imported and Hall / ATCC 3502 / NCTC 13319 / Type A [Sanger]Imported.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HallImported and Hall / ATCC 3502 / NCTC 13319 / Type A [Los Alamos]Imported.
  3. Shrivastava S., Brinkac L.M., Dodson R.J., Harkins D.M., Durkin A.S., Sutton G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: HallImported.

Entry informationi

Entry nameiA5I2C8_CLOBH
AccessioniPrimary (citable) accession number: A5I2C8
Secondary accession number(s): A7G426
Entry historyi
Integrated into UniProtKB/TrEMBL: June 26, 2007
Last sequence update: June 26, 2007
Last modified: April 29, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.