Reviewed, UniProtKB/Swiss-Prot A5I0I2 (ADD_CLOBH)

Names and origin

Protein names

Recommended name:
    Adenosine deaminase
    EC=3.5.4.4
Alternative name(s):
    Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	CBO0991, CLC_1043

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Adenosine deaminase HAMAP MF_00540		PRO_1000017655
Sites
	Active site	197	1	Potential
	Active site	245	1	Potential
	Active site	278	1	Potential
	Active site	279	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A5I0I2-1 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: 4F815579CB190B07	FASTA	335	37,683
	10 20 30 40 50 60 MNFKKLPKIE LHCHLDGSLR IDTILDIAKK DNIPLPSYNK KELINYVSIM DDCNSLDEYL 70 80 90 100 110 120 NKFFIPNKVM QTKENLKRIA FELLEDVAAD NVKYIEVRFA PLLHVEKGLN IEEIIESVLA 130 140 150 160 170 180 GIKEAEKLYD IKGNLILGCM RNMDIPSAFE VVKKGAKFIG KGVVAIDLCA GEEPHFPGKY 190 200 210 220 230 240 IEVLKLAKEC GYRITIHAGE AGVGENVLEA INLLNAERIG HGIYIKNCAE AYKLVKEKNI 250 260 270 280 290 300 PLEVCPTSNL HTKAFESYET HPFMDFLKDG IKVTINTDNM TVSNTTITKE LEMLNKFCGL 310 320 330 SIEDYKILYL NAVEASFASP ETKEILKSYA NEITA

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS37648.1. AM412317 Genomic DNA. Translation: CAL82543.1.
RefSeq	YP_001253522.1. YP_001386911.1.
3D structure databases
SMR	A5I0I2. Positions 5-327.
ModBase	Search...
Protein-protein interaction databases
STRING	A5I0I2.
Genome annotation databases
GeneID	5185246. 5399812.
GenomeReviews	Gene locus CBO0991 in contig AM412317_GR. Gene locus CLC_1043 in contig CP000727_GR.
KEGG	cbh:CLC_1043. cbo:CBO0991.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1816.
HOGENOM	HBG630382.
OMA	FLKKLDW.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase. IPR006330. A_deaminase. [Graphical view]
PANTHER	PTHR11409. A/AMP_deaminase. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_CLOBH

Accession

Primary (citable) accession number: A5I0I2
Secondary accession number(s): A7FZQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 26, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)