A5HZ99 (A5HZ99_CLOBH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel HAMAP MF_00115

Gene names

Name:	mscL HAMAP MF_00115 EMBL CAL82108.1
Ordered Locus Names:	CBO0555, CLC_0611

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family. HAMAP MF_00115

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane SAAS SAAS001185 HAMAP MF_00115 Membrane
Domain	Transmembrane Transmembrane helix SAAS SAAS001185 HAMAP MF_00115
Molecular function	Ionic channel SAAS SAAS001185 HAMAP MF_00115
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Transmembrane	33 – 53	21	Helical; By similarity HAMAP MF_00115
	Transmembrane	78 – 98	21	Helical; By similarity HAMAP MF_00115

Sequences

	Sequence		Length	Mass (Da)
	A5HZ99 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: 2386092CAC59E91C	FASTA	133	14,841
	10 20 30 40 50 60 MKKIIEEFKD FAVKGNAIEL AVGVVIGSAF GKIVTSLVED IIMPLVGLLI GGIDFTGLKF 70 80 90 100 110 120 SMNLSGKTVV SINYGNFIQA AVNFLIISFS IFLFIKLINK FKQNEEEEKD EPKISNEEIL 130 LREIRDLLKE NKS

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A [Sanger].

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A [Los Alamos].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS36331.1. AM412317 Genomic DNA. Translation: CAL82108.1.
RefSeq	YP_001253097.1. NC_009495.1. YP_001386492.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5HZ99.
ModBase	Search...
Protein-protein interaction databases
STRING	A5HZ99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5184810. 5399522.
GenomeReviews	Gene locus CBO0555 in contig AM412317_GR. Gene locus CLC_0611 in contig CP000727_GR.
KEGG	cbh:CLC_0611. cbo:CBO0555.
PATRIC	19363225. VBICloBot22612_0556.
Phylogenomic databases
eggNOG	COG1970.
HOGENOM	HBG727604.
OMA	VIDFVIV.
ProtClustDB	CLSK971647.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5HZ99_CLOBH

Accession

Primary (citable) accession number: A5HZ99
Secondary accession number(s): A7G165

Entry history

Integrated into UniProtKB/TrEMBL:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)