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A5HZ44

- TRMB_CLOBH

UniProt

A5HZ44 - TRMB_CLOBH

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431S-adenosyl-L-methionineUniRule annotation
    Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
    Binding sitei101 – 1011S-adenosyl-L-methionineUniRule annotation
    Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation
    Binding sitei127 – 1271SubstrateUniRule annotation
    Binding sitei159 – 1591SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCBOT413999:GJ72-562-MONOMER.
    CBOT441771:GIWX-545-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:CBO0499, CLC_0573
    OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
    Taxonomic identifieri441771 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferasePRO_1000136346Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi413999.CBO0499.

    Structurei

    3D structure databases

    ProteinModelPortaliA5HZ44.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1346Interaction with RNAUniRule annotation
    Regioni196 – 1994Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiDNVMTEY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5HZ44-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLRKKWWAR PEIEASDKFA EEPKELRGKW NKEFNNNNDI HLELGCGRGG    50
    FISQLVEKNK DINYVGIDLK DEVIVYAIRK VKEKEEEVKR EFKNIKFVTM 100
    NIMGIAEVFD KNEISKIYIN FCNPWPKERH NKRRLTHTKL LTEYKKFLKP 150
    NTEIWFKTDD KELFEDSQEY FKESGFNIEY ITYDLHNSDF KENIKTEYET 200
    KFETMGMKIM FLKARLL 217
    Length:217
    Mass (Da):26,259
    Last modified:June 26, 2007 - v1
    Checksum:iC9CEDFA575906AD2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000727 Genomic DNA. Translation: ABS38078.1.
    AM412317 Genomic DNA. Translation: CAL82053.1.
    RefSeqiYP_001253043.1. NC_009495.1.
    YP_001386456.1. NC_009698.1.

    Genome annotation databases

    EnsemblBacteriaiABS38078; ABS38078; CLC_0573.
    GeneIDi5184754.
    5399680.
    KEGGicbh:CLC_0573.
    cbo:CBO0499.
    PATRICi19363117. VBICloBot22612_0502.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000727 Genomic DNA. Translation: ABS38078.1 .
    AM412317 Genomic DNA. Translation: CAL82053.1 .
    RefSeqi YP_001253043.1. NC_009495.1.
    YP_001386456.1. NC_009698.1.

    3D structure databases

    ProteinModelPortali A5HZ44.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 413999.CBO0499.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS38078 ; ABS38078 ; CLC_0573 .
    GeneIDi 5184754.
    5399680.
    KEGGi cbh:CLC_0573.
    cbo:CBO0499.
    PATRICi 19363117. VBICloBot22612_0502.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi DNVMTEY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci CBOT413999:GJ72-562-MONOMER.
    CBOT441771:GIWX-545-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
    2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
      Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
      PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

    Entry informationi

    Entry nameiTRMB_CLOBH
    AccessioniPrimary (citable) accession number: A5HZ44
    Secondary accession number(s): A7G137
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3