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A5HYZ4 (THIM1_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase 1

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 1
Short name=TH kinase 1
Short name=Thz kinase 1
Gene names
Name:thiM1
Ordered Locus Names:CBO0450, CLC_0524
OrganismClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Reference proteome] [HAMAP]
Taxonomic identifier441771 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Hydroxyethylthiazole kinase 1 HAMAP-Rule MF_00228
PRO_0000336548

Sites

Binding site421Substrate; via amide nitrogen By similarity
Binding site1181ATP By similarity
Binding site1641ATP By similarity
Binding site1911Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5HYZ4 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: C38F86137ECBC3D2

FASTA26328,455
        10         20         30         40         50         60 
MKNKNVIQKM REKTPLIHCI TNYVTINDCA NILLSFGASP AMCEAYDEVY DFVSISSALY 

        70         80         90        100        110        120 
INLGTLTKEQ ETAAVLASIS AKNHNVPVVI DPVGCPAIKR KVEVINRIAE VGRIDIIKGN 

       130        140        150        160        170        180 
IGEIKFLAGM DSETRGVDSL DNGENALDAC TQLAKKYNCI VAATGEKDFV SDGKRGSVIK 

       190        200        210        220        230        240 
NGTEMLTKVT GAGCMLGALC AATCANFEDK LVSTTAAILS MNIAGEKAYE EAQLPGSFRI 

       250        260 
ALIDNIYMIS DEEIWERGNV EWK 

« Hide

References

[1]"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes."
Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. expand/collapse author list , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J.
Genome Res. 17:1082-1092(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
[2]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000727 Genomic DNA. Translation: ABS39081.1.
AM412317 Genomic DNA. Translation: CAL82003.1.
RefSeqYP_001252994.1. NC_009495.1.
YP_001386409.1. NC_009698.1.

3D structure databases

ProteinModelPortalA5HYZ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING413999.CBO0450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS39081; ABS39081; CLC_0524.
GeneID5184705.
5400612.
KEGGcbh:CLC_0524.
cbo:CBO0450.
PATRIC19363017. VBICloBot22612_0453.

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAENGTEML.
OrthoDBEOG628F8M.
ProtClustDBCLSK950618.

Enzyme and pathway databases

BioCycCBOT413999:GJ72-510-MONOMER.
CBOT441771:GIWX-495-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM1_CLOBH
AccessionPrimary (citable) accession number: A5HYZ4
Secondary accession number(s): A7G0Z0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 26, 2007
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways