A5HYC6 (SURE_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-nucleotidase surE
EC=3.1.3.5

Alternative name(s):
Nucleoside 5'-monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	CBO0233, CLC_0289

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates By similarity. HAMAP MF_00060
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00060.
Sequence similarities	Belongs to the surE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	5'-nucleotidase surE HAMAP MF_00060		PRO_1000007722
Sites
	Metal binding	8	1	Divalent metal cation By similarity
	Metal binding	9	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	95	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5HYC6 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: C141981431FBB4A4	FASTA	252	28,322
	10 20 30 40 50 60 MNILLTNDDG IEAEGINTLA ELLSKYHDVI MVAPENQRSA SSHSITIYEP IIVKQVKKPY 70 80 90 100 110 120 NIEAYSISGT PADCVRVALD KLVPDNIDMV ISGINKGLNI GNDILYSGTV SAAIEGAMYK 130 140 150 160 170 180 VPSMAVSAQF IKNKKENYKI AAKYALRMLN RLKKEDLKND VVLNLNIPFC SEEEIKGIKV 190 200 210 220 230 240 CKVGNKIFNT RFSEEIDEEG NKVLKLEGDI NKDIYEGTDV YYIRNKYVTL TPLHYDLTNF 250 NILEETEQLF LS

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed: 17519437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS37099.1. AM412317 Genomic DNA. Translation: CAL81785.1.
RefSeq	YP_001252777.1. NC_009495.1. YP_001386189.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5HYC6.
ModBase	Search...
Protein-protein interaction databases
STRING	A5HYC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5184488. 5399786.
GenomeReviews	Gene locus CBO0233 in contig AM412317_GR. Gene locus CLC_0289 in contig CP000727_GR.
KEGG	cbh:CLC_0289. cbo:CBO0233.
PATRIC	19362561. VBICloBot22612_0231.
Phylogenomic databases
eggNOG	COG0496.
HOGENOM	HBG600532.
OMA	NGFYYVN.
ProtClustDB	PRK13933.
Enzyme and pathway databases
BioCyc	CBOT36826:CBO0233-MONOMER. CBOT441771:CLC_0289-MONOMER.
Family and domain databases
HAMAP	MF_00060. SurE. [Tree]
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Gene3D	G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.
KO	K03787.
Pfam	PF01975. SurE. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. SurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_CLOBH

Accession

Primary (citable) accession number: A5HYC6
Secondary accession number(s): A7G0D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 26, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program