A5HYC0 (PGK_CLOBH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	CBO0227, CLC_0283

Organism

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) [Reference proteome] [HAMAP]

Taxonomic identifier

441771 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum A

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_1000009607
Regions
	Nucleotide binding	354 – 357	4	ATP By similarity
	Region	23 – 25	3	Substrate binding By similarity
	Region	61 – 64	4	Substrate binding By similarity
Sites
	Binding site	38	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	206	1	ATP By similarity
	Binding site	297	1	ATP; via carbonyl oxygen By similarity
	Binding site	328	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5HYC0 [UniParc]. Last modified June 26, 2007. Version 1. Checksum: D2092EB5B08046A8	FASTA	398	43,062
	10 20 30 40 50 60 MNYNKKSIED IDVKGKKVLV RCDFNVPLNE GKITDENRLV GALPTIKYLM EKGAKIILCS 70 80 90 100 110 120 HMGKPKGEPK KELSLLPVAK RLSEMLNKEV IFADDDNVVG ENAKKAVEDM KDGDVVLLQN 130 140 150 160 170 180 TRYRKEETKN EEVFSKELAS LADVFVNDAF GTAHRAHCST VGVTNYLKEA ACGYLIQKEL 190 200 210 220 230 240 KFLGNAVEKP ERPFVAILGG AKVSDKINVI NNLLDKVDTL IIGGGMGYTF LKAQGYTIGN 250 260 270 280 290 300 SLVEEDKVEY SKEMIDKAKE KGVNLLLPID NVVADKFDKD ASPVITEDQN IGEGYMGLDI 310 320 330 340 350 360 GPKTAKIYSD AIKSAKTVVW NGPMGVFEFK SFANGTIEVA KAMADSDAVT IIGGGDSAAA 370 380 390 VNILGFGDKM THISTGGGAS LEFLEGKELP GIAALNDK

References

[1]

"Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. , Feltwell T., Fraser A., Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., White B., Whithead S., Parkhill J. Genome Res. 17:1082-1092(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

[2]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000727 Genomic DNA. Translation: ABS39087.1. AM412317 Genomic DNA. Translation: CAL81779.1.
RefSeq	YP_001252771.1. NC_009495.1. YP_001386183.1. NC_009698.1.
3D structure databases
ProteinModelPortal	A5HYC0.
SMR	A5HYC0. Positions 4-398.
ModBase	Search...
Protein-protein interaction databases
STRING	413999.CBO0227.
Proteomic databases
PRIDE	A5HYC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS39087; ABS39087; CLC_0283.
GeneID	5187988. 5400065.
KEGG	cbh:CLC_0283. cbo:CBO0227.
PATRIC	19362549. VBICloBot22612_0225.
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227108.
KO	K00927.
OMA	EKKNDEA.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	CBOT413999:GJ72-276-MONOMER. CBOT441771:GIWX-259-MONOMER.
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_CLOBH

Accession

Primary (citable) accession number: A5HYC0
Secondary accession number(s): A7FZK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program