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A5HY79

- SYL_CLOBH

UniProt

A5HY79 - SYL_CLOBH

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei575 – 5751ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-231-MONOMER.
CBOT441771:GIWX-217-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CBO0186, CLC_0240
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Leucine--tRNA ligasePRO_1000009326Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO0186.

Structurei

3D structure databases

ProteinModelPortaliA5HY79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi572 – 5765"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHISHELW.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A5HY79-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNYSTKIDE KWQKKWEENS LYKFNNKNLD KKLYVLEMFS YPSGSKLHAG
60 70 80 90 100
HWFNYGPVDS WARFKRMQGY NVFQPMGFDA FGLPAENYAI KTGIHPKDST
110 120 130 140 150
FKNIETMETQ LKAMGAMFNW ENEVITCSPD YYKWTQWLFL KLYEKGLAYK
160 170 180 190 200
KKAPVNWCPS CNTVLANEQV LDGKCERCDS NVDKKNLEQW FLKITDYADE
210 220 230 240 250
LLEKLDELDW PEKTKAMQKH WIGKSVGAEV TFNVADSDLS FNVFTTRVDT
260 270 280 290 300
LFGVTYVVLA PENDLVDKLT TPENKAEVES YKTQAKNQSD IERQSITREK
310 320 330 340 350
TGVFSGSYAI NPINGKKVPI WIGDYVLNTY GTGCVMAVPA HDERDFAFAT
360 370 380 390 400
KYNLPIERVI EGGDSLPYTE YGGMVNSGEF DGLLGNEAKE AVISKLESMN
410 420 430 440 450
LGRKKINYRL RDWLVSRQRY WGAPIPIIYC EKCGTVEVPI EQLPVELPYN
460 470 480 490 500
VEFSPDGKSP LGKCDDFINT TCPKCGGPAK READTLDTFV CSSWYYLRYP
510 520 530 540 550
DNNNEKDAFN PELINKMLPV DKYVGGPEHA CMHLLYARFI TKALRDMGYL
560 570 580 590 600
NFDEPFLSLT HQGLILGPDG LKMSKSKGNT ISPDDYIKEF GADVFRMYLM
610 620 630 640 650
FGFDYTEGGA WSDDAIKSIG KFVDRVERIL ENAREEIKNS KDNKSTMDKD
660 670 680 690 700
EKELNYVRHH SIKSITEDID KMQFNTSIAR LMEFTNALSK YLGIDAIKNA
710 720 730 740 750
LFLRESIIDF ITLLAPFAPH FAEEQWKLIG INSSIFNEKW PEFDPKALIK
760 770 780 790 800
DEVEIAVQVN GKIRAKINIS TSSSEDEIKE SALNNEDIKN SIGDKEIKKV
810
IVIKNRLVNI VAK
Length:813
Mass (Da):92,912
Last modified:June 26, 2007 - v1
Checksum:iF4DECBDF3244DC0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS36860.1.
AM412317 Genomic DNA. Translation: CAL81738.1.
RefSeqiWP_011948005.1. NC_009698.1.
YP_001252730.1. NC_009495.1.
YP_001386143.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36860; ABS36860; CLC_0240.
GeneIDi5187707.
5400866.
KEGGicbh:CLC_0240.
cbo:CBO0186.
PATRICi19362465. VBICloBot22612_0184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS36860.1 .
AM412317 Genomic DNA. Translation: CAL81738.1 .
RefSeqi WP_011948005.1. NC_009698.1.
YP_001252730.1. NC_009495.1.
YP_001386143.1. NC_009698.1.

3D structure databases

ProteinModelPortali A5HY79.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 413999.CBO0186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS36860 ; ABS36860 ; CLC_0240 .
GeneIDi 5187707.
5400866.
KEGGi cbh:CLC_0240.
cbo:CBO0186.
PATRICi 19362465. VBICloBot22612_0184.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200748.
KOi K01869.
OMAi HISHELW.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci CBOT413999:GJ72-231-MONOMER.
CBOT441771:GIWX-217-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiSYL_CLOBH
AccessioniPrimary (citable) accession number: A5HY79
Secondary accession number(s): A7G094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: November 26, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3