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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei575 – 5751ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-231-MONOMER.
CBOT441771:GIWX-217-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CBO0186, CLC_0240
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002409 Componenti: Chromosome UP000001986 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Leucine--tRNA ligasePRO_1000009326Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi413999.CBO0186.

Structurei

3D structure databases

ProteinModelPortaliA5HY79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi572 – 5765"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A5HY79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNYSTKIDE KWQKKWEENS LYKFNNKNLD KKLYVLEMFS YPSGSKLHAG
60 70 80 90 100
HWFNYGPVDS WARFKRMQGY NVFQPMGFDA FGLPAENYAI KTGIHPKDST
110 120 130 140 150
FKNIETMETQ LKAMGAMFNW ENEVITCSPD YYKWTQWLFL KLYEKGLAYK
160 170 180 190 200
KKAPVNWCPS CNTVLANEQV LDGKCERCDS NVDKKNLEQW FLKITDYADE
210 220 230 240 250
LLEKLDELDW PEKTKAMQKH WIGKSVGAEV TFNVADSDLS FNVFTTRVDT
260 270 280 290 300
LFGVTYVVLA PENDLVDKLT TPENKAEVES YKTQAKNQSD IERQSITREK
310 320 330 340 350
TGVFSGSYAI NPINGKKVPI WIGDYVLNTY GTGCVMAVPA HDERDFAFAT
360 370 380 390 400
KYNLPIERVI EGGDSLPYTE YGGMVNSGEF DGLLGNEAKE AVISKLESMN
410 420 430 440 450
LGRKKINYRL RDWLVSRQRY WGAPIPIIYC EKCGTVEVPI EQLPVELPYN
460 470 480 490 500
VEFSPDGKSP LGKCDDFINT TCPKCGGPAK READTLDTFV CSSWYYLRYP
510 520 530 540 550
DNNNEKDAFN PELINKMLPV DKYVGGPEHA CMHLLYARFI TKALRDMGYL
560 570 580 590 600
NFDEPFLSLT HQGLILGPDG LKMSKSKGNT ISPDDYIKEF GADVFRMYLM
610 620 630 640 650
FGFDYTEGGA WSDDAIKSIG KFVDRVERIL ENAREEIKNS KDNKSTMDKD
660 670 680 690 700
EKELNYVRHH SIKSITEDID KMQFNTSIAR LMEFTNALSK YLGIDAIKNA
710 720 730 740 750
LFLRESIIDF ITLLAPFAPH FAEEQWKLIG INSSIFNEKW PEFDPKALIK
760 770 780 790 800
DEVEIAVQVN GKIRAKINIS TSSSEDEIKE SALNNEDIKN SIGDKEIKKV
810
IVIKNRLVNI VAK
Length:813
Mass (Da):92,912
Last modified:June 26, 2007 - v1
Checksum:iF4DECBDF3244DC0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS36860.1.
AM412317 Genomic DNA. Translation: CAL81738.1.
RefSeqiWP_011948005.1. NC_009698.1.
YP_001252730.1. NC_009495.1.
YP_001386143.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS36860; ABS36860; CLC_0240.
GeneIDi5187707.
5400866.
KEGGicbh:CLC_0240.
cbo:CBO0186.
PATRICi19362465. VBICloBot22612_0184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS36860.1.
AM412317 Genomic DNA. Translation: CAL81738.1.
RefSeqiWP_011948005.1. NC_009698.1.
YP_001252730.1. NC_009495.1.
YP_001386143.1. NC_009698.1.

3D structure databases

ProteinModelPortaliA5HY79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi413999.CBO0186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS36860; ABS36860; CLC_0240.
GeneIDi5187707.
5400866.
KEGGicbh:CLC_0240.
cbo:CBO0186.
PATRICi19362465. VBICloBot22612_0184.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-231-MONOMER.
CBOT441771:GIWX-217-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiSYL_CLOBH
AccessioniPrimary (citable) accession number: A5HY79
Secondary accession number(s): A7G094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: February 4, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.