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Protein

Thymidine kinase

Gene

tdk

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Proton acceptorUniRule annotation
Metal bindingi145 – 1451ZincUniRule annotation
Metal bindingi148 – 1481ZincUniRule annotation
Metal bindingi183 – 1831ZincUniRule annotation
Metal bindingi186 – 1861ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATPUniRule annotation
Nucleotide bindingi88 – 914ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-174-MONOMER.
CBOT441771:GIWX-164-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:tdkUniRule annotation
Ordered Locus Names:CBO0136, CLC_0184
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001986: Chromosome, UP000002409: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Thymidine kinasePRO_1000018153Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi413999.CBO0136.

Structurei

3D structure databases

ProteinModelPortaliA5HY32.
SMRiA5HY32. Positions 2-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076390.
KOiK00857.
OMAiVITGPMY.
OrthoDBiEOG69D3J2.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5HY32-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYGPKDHGWI EVVAGPMYSG KTEELIRRIR RAEIAKQKVQ VFKPEIDNRY
60 70 80 90 100
SKQDVVSHAG DKIQSVPVKS SKEILEKLLD DTDVIGIDEA QFFDDFLVEI
110 120 130 140 150
VSKIANNNRR VICAGLDMDF KGEPFGPMPK LMAIAEFVDK IQAVCMVCNN
160 170 180 190
PATRTQRLIN GKPAKKSDPV VLIGAQESYE ARCRKCHRVP R
Length:191
Mass (Da):21,545
Last modified:June 26, 2007 - v1
Checksum:i524B3EA8664BD59E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS37958.1.
AM412317 Genomic DNA. Translation: CAL81691.1.
RefSeqiYP_001252683.1. NC_009495.1.
YP_001386095.1. NC_009698.1.

Genome annotation databases

EnsemblBacteriaiABS37958; ABS37958; CLC_0184.
GeneIDi5184391.
5399142.
KEGGicbh:CLC_0184.
cbo:CBO0136.
PATRICi19362357. VBICloBot22612_0135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA. Translation: ABS37958.1.
AM412317 Genomic DNA. Translation: CAL81691.1.
RefSeqiYP_001252683.1. NC_009495.1.
YP_001386095.1. NC_009698.1.

3D structure databases

ProteinModelPortaliA5HY32.
SMRiA5HY32. Positions 2-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi413999.CBO0136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS37958; ABS37958; CLC_0184.
GeneIDi5184391.
5399142.
KEGGicbh:CLC_0184.
cbo:CBO0136.
PATRICi19362357. VBICloBot22612_0135.

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076390.
KOiK00857.
OMAiVITGPMY.
OrthoDBiEOG69D3J2.

Enzyme and pathway databases

BioCyciCBOT413999:GJ72-174-MONOMER.
CBOT441771:GIWX-164-MONOMER.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.
  2. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hall / ATCC 3502 / NCTC 13319 / Type A.

Entry informationi

Entry nameiKITH_CLOBH
AccessioniPrimary (citable) accession number: A5HY32
Secondary accession number(s): A7G052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.