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A5HXZ6 (A5HXZ6_CLOBH) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase 1 HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase 1 HAMAP MF_02003
Gene names
Name:ileS1 HAMAP MF_02003
Ordered Locus Names:CBO0099
OrganismClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifier441771 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length1040 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
A5HXZ6 [UniParc].

Last modified June 26, 2007. Version 1.
Checksum: 0AEA25F1AD460F7C

FASTA1,040120,581
        10         20         30         40         50         60 
MGMYNKIDSN KSFVQREKGI ADLWKDRGVI EKSFNLNEDG EYFTFYDGPP TANGKPHVGH 

        70         80         90        100        110        120 
ILTRVMKDLI PRYKVMKGYK VLRKAGWDTH GLPVELEIEK KLGISGKPQI EEYGIEKFVT 

       130        140        150        160        170        180 
ECKDSVFKYT SLWKQMSEQL GFWVDMDDPY ITYDNNYIES VWWALKQMWK KELLYKGHRV 

       190        200        210        220        230        240 
TPYCPRCGTA LSSHEVAQGY KDVKEATAFV KFKIKGKENK YFLAWTTTPW TLPSNVTLAI 

       250        260        270        280        290        300 
NKSYDYVEIL NNDEHYILAK ELLGKVIEGE YEVVKEFKGE EIVGVEYEQL FKFEVPEKKA 

       310        320        330        340        350        360 
FYVVHADYVT LTDGTGIVHT APAYGDDDSK TGKKYDLPLI NLVDSEGKFV DSVEPWKGMF 

       370        380        390        400        410        420 
VKKADPKILE YLKENGMLYK SEKFTHSYPH CWRCNTPLLY YPKDSWFVRM TSLRDDLVKN 

       430        440        450        460        470        480 
NNTINWYPDN IRTGRFGKFV ENVIDWGISR DRYWGTPLPI WECDCGHREC IGSVEELKEK 

       490        500        510        520        530        540 
GINVPENIEL HKPYIDAVKL NCPHCGKEMT RTNEVIDCWF DSGSMPFAQH HYPFENKEVF 

       550        560        570        580        590        600 
ENTFPAQFIS EAVDQTRGWF YTLLAISTAL FGKSSYENCI VLGHVLDKHG LKMSKSKGNV 

       610        620        630        640        650        660 
VDPFDVLENE GADATRWHFY TASAPWLPTR FSEEDVKETQ RKFLSTLWNV YSFYILYADL 

       670        680        690        700        710        720 
DNFNPLEYKE FKSEHVMDKW ILSKLNSLIK NVEEHLDNYR ITQAALELEE FVDELSNWYV 

       730        740        750        760        770        780 
RRNRSRFWST ELTDDKIGAY TTLYTVLTTV IKVAAPFVPF VTEEMYQNLV VNLDKDAEES 

       790        800        810        820        830        840 
IHLCKWPSYN ESTMDKDLEE KMGLAYKIVK LGRSARNSVN IKNRQPLKKM LVSTKELPEY 

       850        860        870        880        890        900 
YEDIIREELN IKEILSGADL SDYVNFEIKP NLPILGKKYG KFIPAIRKEI SSMNQMELAQ 

       910        920        930        940        950        960 
SINNSRSVFI NVEGCEDQIE LTAENLLVTM QGLEGFAFAG EGEIGVVLDT HITEELKEEG 

       970        980        990       1000       1010       1020 
FLREILSKVQ NMRKESGFEV ADKINLYVSG NNTLNAIVKK YEDEIKVETL SVKVMYDENK 

      1030       1040 
DYIECKINGE NYNIAMEVVK

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM412317 Genomic DNA. Translation: CAL81655.1.
RefSeqYP_001252647.1. NC_009495.1.

3D structure databases

ProteinModelPortalA5HXZ6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5HXZ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5184354.
GenomeReviewsGene locus CBO0099 in contig AM412317_GR.
KEGGcbo:CBO0099.
PATRIC19362285. VBICloBot22612_0099.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA5HXZ6_CLOBH
AccessionPrimary (citable) accession number: A5HXZ6
Entry history
Integrated into UniProtKB/TrEMBL: June 26, 2007
Last sequence update: June 26, 2007
Last modified: January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info