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A5HII1

- ACTN_ACTDE

UniProt

A5HII1 - ACTN_ACTDE

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Protein

Actinidain

Gene
N/A
Organism
Actinidia deliciosa (Kiwi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cysteine protease responsible for the cleavage of kiwellin into kissper and KiTH.1 Publication

Catalytic activityi

Specificity close to that of papain.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511By similarity
Active sitei288 – 2881By similarity
Active sitei308 – 3081By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Names & Taxonomyi

Protein namesi
Recommended name:
ActinidainImportedBy similarity (EC:3.4.22.14)
Short name:
ActinidinImportedBy similarity
Alternative name(s):
Allergen Act d 1
Allergen: Act d 1
OrganismiActinidia deliciosa (Kiwi)
Taxonomic identifieri3627 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds IgE.2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1. Act d 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 126102Activation peptide3 PublicationsPRO_0000343461Add
BLAST
Chaini127 – 380254Actinidain1 PublicationPRO_0000343462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi148 ↔ 191By similarity
Disulfide bondi182 ↔ 224By similarity
Disulfide bondi282 ↔ 332By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Fruit, present in small cells of the outer pericarp of mature fruit, but not large cells.1 Publication

Developmental stagei

Expressed in ripening fruit, levels are highest at the harvest of fruit and decrease as the fruit ripens.1 Publication

Structurei

3D structure databases

ProteinModelPortaliA5HII1.
SMRiA5HII1. Positions 127-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5HII1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLPKSFVSM SLLFFSTLLI LSLAFNAKNL TQRTNDEVKA MYESWLIKYG
60 70 80 90 100
KSYNSLGEWE RRFEIFKETL RFIDEHNADT NRSYKVGLNQ FADLTDEEFR
110 120 130 140 150
STYLGFTSGS NKTKVSNRYE PRVGQVLPSY VDWRSAGAVV DIKSQGECGG
160 170 180 190 200
CWAFSAIATV EGINKIVTGV LISLSEQELI DCGRTQNTRG CNGGYITDGF
210 220 230 240 250
QFIINNGGIN TEENYPYTAQ DGECNLDLQN EKYVTIDTYE NVPYNNEWAL
260 270 280 290 300
QTAVTYQPVS VALDAAGDAF KHYSSGIFTG PCGTAIDHAV TIVGYGTEGG
310 320 330 340 350
IDYWIVKNSW DTTWGEEGYM RILRNVGGAG TCGIATMPSY PVKYNNQNHP
360 370 380
KPYSSLINPP AFSMSKDGPV GVDDGQRYSA
Length:380
Mass (Da):42,110
Last modified:June 12, 2007 - v1
Checksum:i70FDAD2235388224
GO

Sequence cautioni

The sequence AAA32630.1 differs from that shown. Reason: Frameshift at position 371.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961D → G in AAA32630. 1 PublicationCurated
Sequence conflicti108 – 1081S → G in AAA32630. 1 PublicationCurated
Sequence conflicti123 – 1231V → F in AAA32630. 1 PublicationCurated
Sequence conflicti124 – 1241G → S in AAA32630. 1 PublicationCurated
Sequence conflicti181 – 1811D → G in AAA32630. 1 PublicationCurated
Sequence conflicti184 – 1841R → G in AAA32630. 1 PublicationCurated
Sequence conflicti212 – 2121E → G in AAA32630. 1 PublicationCurated
Sequence conflicti226 – 2272LD → VE in AAA32629. (PubMed:2251128)Curated
Sequence conflicti240 – 2401E → G in AAA32630. 1 PublicationCurated
Sequence conflicti272 – 2721H → Q in AAA32629. (PubMed:2251128)Curated
Sequence conflicti307 – 3071K → E in AAA32630. 1 PublicationCurated
Sequence conflicti349 – 3491H → Y in AAA32629. (PubMed:2251128)Curated
Sequence conflicti349 – 3491H → Y in AAA32630. 1 PublicationCurated
Sequence conflicti351 – 3511K → E in AAA32629. (PubMed:2251128)Curated
Sequence conflicti360 – 3601P → S in AAA32630. 1 PublicationCurated
Sequence conflicti373 – 3731D → E in AAA32629. (PubMed:2251128)Curated
Sequence conflicti373 – 3731D → E in AAA32630. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16466 mRNA. Translation: CAA34486.1.
M38422 Genomic DNA. Translation: AAA32629.1.
EF530131 mRNA. Translation: ABQ10189.1.
X57551 Genomic DNA. Translation: CAA40778.1.
M21335 mRNA. Translation: AAA32630.1. Frameshift.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16466 mRNA. Translation: CAA34486.1 .
M38422 Genomic DNA. Translation: AAA32629.1 .
EF530131 mRNA. Translation: ABQ10189.1 .
X57551 Genomic DNA. Translation: CAA40778.1 .
M21335 mRNA. Translation: AAA32630.1 . Frameshift.

3D structure databases

ProteinModelPortali A5HII1.
SMRi A5HII1. Positions 127-343.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 1. Act d 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view ]
PANTHERi PTHR12411. PTHR12411. 1 hit.
Pfami PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view ]
PRINTSi PR00705. PAPAIN.
SMARTi SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view ]
PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of actinidin, a kiwi fruit protease."
    Podivinsky E., Forster R.L.S., Gardner R.C.
    Nucleic Acids Res. 17:8363-8363(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nucleotide sequence of an actinidin genomic clone."
    Snowden K.C., Gardner R.C.
    Nucleic Acids Res. 18:6684-6684(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. HaywardImported.
  3. "Identification and characterisation of acidic and novel basic forms of actinidin, the highly abundant cysteine protease from kiwifruit."
    Nieuwenhuizen N.J., Beuning L.L., Sutherland P.W., Sharma N.N., Cooney J.M., Bieleski L.R.F., Schroeder R., MacRae E.A., Atkinson R.G.
    Funct. Plant Biol. 34:946-961(2007)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. Hayward1 Publication.
  4. "Nucleotide sequence of the promoter region from kiwifruit actinidin genes."
    Keeling J., Maxwell P., Gardner R.C.
    Plant Mol. Biol. 15:787-788(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
  5. Praekelt U.M., McKee R.A., Smith H.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-380.
    Strain: cv. Exbury.
  6. "Kiwellin, a modular protein from green and gold kiwi fruits: evidence of in vivo and in vitro processing and IgE binding."
    Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V., Tamburrini M., Mari A., Ciardiello M.A.
    J. Agric. Food Chem. 56:3812-3817(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 127-136, FUNCTION.
    Strain: cv. Hayward1 Publication.
    Tissue: Fruit1 Publication.
  7. "IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries."
    Bublin M., Mari A., Ebner C., Knulst A., Scheiner O., Hoffmann-Sommergruber K., Breiteneder H., Radauer C.
    J. Allergy Clin. Immunol. 114:1169-1175(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 127-131, ALLERGEN.
    Strain: cv. Hayward1 Publication.
    Tissue: Fruit1 Publication.
  8. "Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms."
    Palacin A., Rodriguez J., Blanco C., Lopez-Torrejon G., Sanchez-Monge R., Varela J., Jimenez M.A., Cumplido J., Carrillo T., Crespo J.F., Salcedo G.
    Clin. Exp. Allergy 38:1220-1228(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 127-131, ALLERGEN.
    Strain: cv. Hayward1 Publication.
    Tissue: Fruit1 Publication.

Entry informationi

Entry nameiACTN_ACTDE
AccessioniPrimary (citable) accession number: A5HII1
Secondary accession number(s): Q43367, Q96227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 12, 2007
Last modified: October 1, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3