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A5HII1

- ACTN_ACTDE

UniProt

A5HII1 - ACTN_ACTDE

Protein

Actinidain

Gene
N/A
Organism
Actinidia deliciosa (Kiwi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Cysteine protease responsible for the cleavage of kiwellin into kissper and KiTH.1 Publication

    Catalytic activityi

    Specificity close to that of papain.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei151 – 1511By similarity
    Active sitei288 – 2881By similarity
    Active sitei308 – 3081By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ActinidainImportedBy similarity (EC:3.4.22.14)
    Short name:
    ActinidinImportedBy similarity
    Alternative name(s):
    Allergen Act d 1
    Allergen: Act d 1
    OrganismiActinidia deliciosa (Kiwi)
    Taxonomic identifieri3627 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds IgE.2 Publications

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei1. Act d 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 126102Activation peptide3 PublicationsPRO_0000343461Add
    BLAST
    Chaini127 – 380254Actinidain1 PublicationPRO_0000343462Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi148 ↔ 191By similarity
    Disulfide bondi182 ↔ 224By similarity
    Disulfide bondi282 ↔ 332By similarity

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Fruit, present in small cells of the outer pericarp of mature fruit, but not large cells.1 Publication

    Developmental stagei

    Expressed in ripening fruit, levels are highest at the harvest of fruit and decrease as the fruit ripens.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliA5HII1.
    SMRiA5HII1. Positions 127-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view]
    PANTHERiPTHR12411. PTHR12411. 1 hit.
    PfamiPF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00705. PAPAIN.
    SMARTiSM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view]
    PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5HII1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLPKSFVSM SLLFFSTLLI LSLAFNAKNL TQRTNDEVKA MYESWLIKYG    50
    KSYNSLGEWE RRFEIFKETL RFIDEHNADT NRSYKVGLNQ FADLTDEEFR 100
    STYLGFTSGS NKTKVSNRYE PRVGQVLPSY VDWRSAGAVV DIKSQGECGG 150
    CWAFSAIATV EGINKIVTGV LISLSEQELI DCGRTQNTRG CNGGYITDGF 200
    QFIINNGGIN TEENYPYTAQ DGECNLDLQN EKYVTIDTYE NVPYNNEWAL 250
    QTAVTYQPVS VALDAAGDAF KHYSSGIFTG PCGTAIDHAV TIVGYGTEGG 300
    IDYWIVKNSW DTTWGEEGYM RILRNVGGAG TCGIATMPSY PVKYNNQNHP 350
    KPYSSLINPP AFSMSKDGPV GVDDGQRYSA 380
    Length:380
    Mass (Da):42,110
    Last modified:June 12, 2007 - v1
    Checksum:i70FDAD2235388224
    GO

    Sequence cautioni

    The sequence AAA32630.1 differs from that shown. Reason: Frameshift at position 371.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 961D → G in AAA32630. 1 PublicationCurated
    Sequence conflicti108 – 1081S → G in AAA32630. 1 PublicationCurated
    Sequence conflicti123 – 1231V → F in AAA32630. 1 PublicationCurated
    Sequence conflicti124 – 1241G → S in AAA32630. 1 PublicationCurated
    Sequence conflicti181 – 1811D → G in AAA32630. 1 PublicationCurated
    Sequence conflicti184 – 1841R → G in AAA32630. 1 PublicationCurated
    Sequence conflicti212 – 2121E → G in AAA32630. 1 PublicationCurated
    Sequence conflicti226 – 2272LD → VE in AAA32629. (PubMed:2251128)Curated
    Sequence conflicti240 – 2401E → G in AAA32630. 1 PublicationCurated
    Sequence conflicti272 – 2721H → Q in AAA32629. (PubMed:2251128)Curated
    Sequence conflicti307 – 3071K → E in AAA32630. 1 PublicationCurated
    Sequence conflicti349 – 3491H → Y in AAA32629. (PubMed:2251128)Curated
    Sequence conflicti349 – 3491H → Y in AAA32630. 1 PublicationCurated
    Sequence conflicti351 – 3511K → E in AAA32629. (PubMed:2251128)Curated
    Sequence conflicti360 – 3601P → S in AAA32630. 1 PublicationCurated
    Sequence conflicti373 – 3731D → E in AAA32629. (PubMed:2251128)Curated
    Sequence conflicti373 – 3731D → E in AAA32630. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16466 mRNA. Translation: CAA34486.1.
    M38422 Genomic DNA. Translation: AAA32629.1.
    EF530131 mRNA. Translation: ABQ10189.1.
    X57551 Genomic DNA. Translation: CAA40778.1.
    M21335 mRNA. Translation: AAA32630.1. Frameshift.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16466 mRNA. Translation: CAA34486.1 .
    M38422 Genomic DNA. Translation: AAA32629.1 .
    EF530131 mRNA. Translation: ABQ10189.1 .
    X57551 Genomic DNA. Translation: CAA40778.1 .
    M21335 mRNA. Translation: AAA32630.1 . Frameshift.

    3D structure databases

    ProteinModelPortali A5HII1.
    SMRi A5HII1. Positions 127-343.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 1. Act d 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view ]
    PANTHERi PTHR12411. PTHR12411. 1 hit.
    Pfami PF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00705. PAPAIN.
    SMARTi SM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view ]
    PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of actinidin, a kiwi fruit protease."
      Podivinsky E., Forster R.L.S., Gardner R.C.
      Nucleic Acids Res. 17:8363-8363(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Nucleotide sequence of an actinidin genomic clone."
      Snowden K.C., Gardner R.C.
      Nucleic Acids Res. 18:6684-6684(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. HaywardImported.
    3. "Identification and characterisation of acidic and novel basic forms of actinidin, the highly abundant cysteine protease from kiwifruit."
      Nieuwenhuizen N.J., Beuning L.L., Sutherland P.W., Sharma N.N., Cooney J.M., Bieleski L.R.F., Schroeder R., MacRae E.A., Atkinson R.G.
      Funct. Plant Biol. 34:946-961(2007)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: cv. Hayward1 Publication.
    4. "Nucleotide sequence of the promoter region from kiwifruit actinidin genes."
      Keeling J., Maxwell P., Gardner R.C.
      Plant Mol. Biol. 15:787-788(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
    5. Praekelt U.M., McKee R.A., Smith H.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-380.
      Strain: cv. Exbury.
    6. "Kiwellin, a modular protein from green and gold kiwi fruits: evidence of in vivo and in vitro processing and IgE binding."
      Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V., Tamburrini M., Mari A., Ciardiello M.A.
      J. Agric. Food Chem. 56:3812-3817(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-136, FUNCTION.
      Strain: cv. Hayward1 Publication.
      Tissue: Fruit1 Publication.
    7. "IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries."
      Bublin M., Mari A., Ebner C., Knulst A., Scheiner O., Hoffmann-Sommergruber K., Breiteneder H., Radauer C.
      J. Allergy Clin. Immunol. 114:1169-1175(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-131, ALLERGEN.
      Strain: cv. Hayward1 Publication.
      Tissue: Fruit1 Publication.
    8. "Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms."
      Palacin A., Rodriguez J., Blanco C., Lopez-Torrejon G., Sanchez-Monge R., Varela J., Jimenez M.A., Cumplido J., Carrillo T., Crespo J.F., Salcedo G.
      Clin. Exp. Allergy 38:1220-1228(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-131, ALLERGEN.
      Strain: cv. Hayward1 Publication.
      Tissue: Fruit1 Publication.

    Entry informationi

    Entry nameiACTN_ACTDE
    AccessioniPrimary (citable) accession number: A5HII1
    Secondary accession number(s): Q43367, Q96227
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3