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A5HII1

- ACTN_ACTDE

UniProt

A5HII1 - ACTN_ACTDE

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Protein
Actinidain
Gene
N/A
Organism
Actinidia deliciosa (Kiwi)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cysteine protease responsible for the cleavage of kiwellin into kissper and KiTH.1 Publication

Catalytic activityi

Specificity close to that of papain.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511 By similarityBy similarity
Active sitei288 – 2881 By similarityBy similarity
Active sitei308 – 3081 By similarityBy similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actinidain (EC:3.4.22.14)
    Short name:
    Actinidin
    Alternative name(s):
    Allergen Act d 1
    Allergen: Act d 1
    OrganismiActinidia deliciosa (Kiwi)
    Taxonomic identifieri3627 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds IgE.2 Publications

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei1. Act d 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424 Reviewed prediction
    Add
    BLAST
    Propeptidei25 – 126102Activation peptide1 Publication
    PRO_0000343461Add
    BLAST
    Chaini127 – 380254Actinidain1 Publication
    PRO_0000343462Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi148 ↔ 191 By similarityBy similarity
    Disulfide bondi182 ↔ 224 By similarityBy similarity
    Disulfide bondi282 ↔ 332 By similarityBy similarity

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Fruit, present in small cells of the outer pericarp of mature fruit, but not large cells.1 Publication

    Developmental stagei

    Expressed in ripening fruit, levels are highest at the harvest of fruit and decrease as the fruit ripens.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliA5HII1.
    SMRiA5HII1. Positions 127-343.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C1 family.

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view]
    PANTHERiPTHR12411. PTHR12411. 1 hit.
    PfamiPF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00705. PAPAIN.
    SMARTiSM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view]
    PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5HII1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLPKSFVSM SLLFFSTLLI LSLAFNAKNL TQRTNDEVKA MYESWLIKYG    50
    KSYNSLGEWE RRFEIFKETL RFIDEHNADT NRSYKVGLNQ FADLTDEEFR 100
    STYLGFTSGS NKTKVSNRYE PRVGQVLPSY VDWRSAGAVV DIKSQGECGG 150
    CWAFSAIATV EGINKIVTGV LISLSEQELI DCGRTQNTRG CNGGYITDGF 200
    QFIINNGGIN TEENYPYTAQ DGECNLDLQN EKYVTIDTYE NVPYNNEWAL 250
    QTAVTYQPVS VALDAAGDAF KHYSSGIFTG PCGTAIDHAV TIVGYGTEGG 300
    IDYWIVKNSW DTTWGEEGYM RILRNVGGAG TCGIATMPSY PVKYNNQNHP 350
    KPYSSLINPP AFSMSKDGPV GVDDGQRYSA 380
    Length:380
    Mass (Da):42,110
    Last modified:June 12, 2007 - v1
    Checksum:i70FDAD2235388224
    GO

    Sequence cautioni

    The sequence AAA32630.1 differs from that shown. Reason: Frameshift at position 371.

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 961D → G in AAA32630. 1 Publication
    Sequence conflicti108 – 1081S → G in AAA32630. 1 Publication
    Sequence conflicti123 – 1231V → F in AAA32630. 1 Publication
    Sequence conflicti124 – 1241G → S in AAA32630. 1 Publication
    Sequence conflicti181 – 1811D → G in AAA32630. 1 Publication
    Sequence conflicti184 – 1841R → G in AAA32630. 1 Publication
    Sequence conflicti212 – 2121E → G in AAA32630. 1 Publication
    Sequence conflicti226 – 2272LD → VE in AAA32629. 1 Publication
    Sequence conflicti240 – 2401E → G in AAA32630. 1 Publication
    Sequence conflicti272 – 2721H → Q in AAA32629. 1 Publication
    Sequence conflicti307 – 3071K → E in AAA32630. 1 Publication
    Sequence conflicti349 – 3491H → Y in AAA32629. 1 Publication
    Sequence conflicti349 – 3491H → Y in AAA32630. 1 Publication
    Sequence conflicti351 – 3511K → E in AAA32629. 1 Publication
    Sequence conflicti360 – 3601P → S in AAA32630. 1 Publication
    Sequence conflicti373 – 3731D → E in AAA32629. 1 Publication
    Sequence conflicti373 – 3731D → E in AAA32630. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16466 mRNA. Translation: CAA34486.1.
    M38422 Genomic DNA. Translation: AAA32629.1.
    EF530131 mRNA. Translation: ABQ10189.1.
    X57551 Genomic DNA. Translation: CAA40778.1.
    M21335 mRNA. Translation: AAA32630.1. Frameshift.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16466 mRNA. Translation: CAA34486.1 .
    M38422 Genomic DNA. Translation: AAA32629.1 .
    EF530131 mRNA. Translation: ABQ10189.1 .
    X57551 Genomic DNA. Translation: CAA40778.1 .
    M21335 mRNA. Translation: AAA32630.1 . Frameshift.

    3D structure databases

    ProteinModelPortali A5HII1.
    SMRi A5HII1. Positions 127-343.
    ModBasei Search...

    Protein family/group databases

    Allergomei 1. Act d 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view ]
    PANTHERi PTHR12411. PTHR12411. 1 hit.
    Pfami PF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00705. PAPAIN.
    SMARTi SM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view ]
    PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of actinidin, a kiwi fruit protease."
      Podivinsky E., Forster R.L.S., Gardner R.C.
      Nucleic Acids Res. 17:8363-8363(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Nucleotide sequence of an actinidin genomic clone."
      Snowden K.C., Gardner R.C.
      Nucleic Acids Res. 18:6684-6684(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Hayward.
    3. "Identification and characterisation of acidic and novel basic forms of actinidin, the highly abundant cysteine protease from kiwifruit."
      Nieuwenhuizen N.J., Beuning L.L., Sutherland P.W., Sharma N.N., Cooney J.M., Bieleski L.R.F., Schroeder R., MacRae E.A., Atkinson R.G.
      Funct. Plant Biol. 34:946-961(2007)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: cv. Hayward.
    4. "Nucleotide sequence of the promoter region from kiwifruit actinidin genes."
      Keeling J., Maxwell P., Gardner R.C.
      Plant Mol. Biol. 15:787-788(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
    5. Praekelt U.M., McKee R.A., Smith H.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-380.
      Strain: cv. Exbury.
    6. "Kiwellin, a modular protein from green and gold kiwi fruits: evidence of in vivo and in vitro processing and IgE binding."
      Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V., Tamburrini M., Mari A., Ciardiello M.A.
      J. Agric. Food Chem. 56:3812-3817(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-136, FUNCTION.
      Strain: cv. Hayward.
      Tissue: Fruit.
    7. "IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries."
      Bublin M., Mari A., Ebner C., Knulst A., Scheiner O., Hoffmann-Sommergruber K., Breiteneder H., Radauer C.
      J. Allergy Clin. Immunol. 114:1169-1175(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-131, ALLERGEN.
      Strain: cv. Hayward.
      Tissue: Fruit.
    8. "Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms."
      Palacin A., Rodriguez J., Blanco C., Lopez-Torrejon G., Sanchez-Monge R., Varela J., Jimenez M.A., Cumplido J., Carrillo T., Crespo J.F., Salcedo G.
      Clin. Exp. Allergy 38:1220-1228(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 127-131, ALLERGEN.
      Strain: cv. Hayward.
      Tissue: Fruit.

    Entry informationi

    Entry nameiACTN_ACTDE
    AccessioniPrimary (citable) accession number: A5HII1
    Secondary accession number(s): Q43367, Q96227
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: June 12, 2007
    Last modified: May 14, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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