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Protein

Ribonuclease-like 3

Gene

rnasel3

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ribonuclease. Angiogenic. Plays a role in host defense. Exhibits strong antibacterial activity against Gram-negative bacteria but mild antibacterial activity against Gram-positive bacteria. The RNase activity is not required for the bactericidal activity.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38Proton acceptor1 Publication1
Active sitei142Proton donor1 Publication1

GO - Molecular functioni

  • endonuclease activity Source: UniProtKB-KW
  • ribonuclease activity Source: ZFIN
  • RNA binding Source: ZFIN

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Developmental protein, Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Angiogenesis, Differentiation, Immunity

Enzyme and pathway databases

BRENDAi3.1.27.5. 928.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease-like 31 Publication (EC:3.1.27.-)
Short name:
RNase ZF-31 Publication
Short name:
RNase-like 31 Publication
Short name:
ZF-RNase-31 Publication
Alternative name(s):
Dr-RNase 11 Publication
Cleaved into the following 2 chains:
N-terminal peptide1 Publication
LF-ZF31 Publication
Gene namesi
Name:rnasel3Imported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050809-5. rnasel3.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000039440423 – 149Ribonuclease-like 3Sequence analysisAdd BLAST127
PeptideiPRO_000039440523 – 55N-terminal peptide1 PublicationAdd BLAST33
PeptideiPRO_000039440656 – 149LF-ZF31 PublicationAdd BLAST94

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi48 ↔ 1091 Publication
Disulfide bondi66 ↔ 1201 Publication
Disulfide bondi84 ↔ 1351 Publication

Post-translational modificationi

Cleavage between Arg-55 and Arg-56 is catalyzed by a membrane-localized Gram-negative bacterium protease (OmpT in E.coli). The excised fragment is then transported to the bacterium cytosol for cleavage of the disulfide bridge linking Cys-48 and Cys-109, thus separating the N-terminal and LF-ZF3. LF-ZF3 but not the N-terminal peptide possesses bactericidal activity.1 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiA5HAK0.

Expressioni

Tissue specificityi

Strongly expressed in the adult liver and gut, and weakly in the heart and testis.1 Publication

Developmental stagei

Only expressed in adults.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000123763.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi28 – 38Combined sources11
Helixi48 – 54Combined sources7
Beta strandi58 – 63Combined sources6
Beta strandi68 – 75Combined sources8
Helixi77 – 81Combined sources5
Helixi82 – 84Combined sources3
Beta strandi87 – 90Combined sources4
Beta strandi92 – 94Combined sources3
Beta strandi97 – 102Combined sources6
Beta strandi104 – 113Combined sources10
Beta strandi121 – 136Combined sources16
Beta strandi139 – 147Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VQ9X-ray1.85A23-149[»]
SMRiA5HAK0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA5HAK0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 71Substrate binding1 Publication5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZBW. Eukaryota.
ENOG410Y4FD. LUCA.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiA5HAK0.
KOiK16631.
PhylomeDBiA5HAK0.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5HAK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIHQCTAVV LLLLCASLST YGQPAEIRRR YEHFLTQHVY GGITEQTCDR
60 70 80 90 100
VMRQRRITRF PTGNDCKEVN TFIQANGNHV RTVCTGGGTR QTDNRDLYMS
110 120 130 140
NNQFTVITCT LRSGERHPNC RYRGKESSRK IVVACEGEWP THYEKGVIV
Length:149
Mass (Da):17,011
Last modified:June 15, 2010 - v2
Checksum:i096E21A32D5B6479
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25A → M no nucleotide entry (PubMed:16861230).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti5Q → R in allele ZF-3d and allele ZF-3e. 1 Publication1
Natural varianti56R → G in allele ZF-3a and allele ZF-3c. 2 Publications1
Natural varianti93D → E in allele ZF-3c. 1 Publication1
Natural varianti102N → D in allele ZF-3e. 1 Publication1
Natural varianti137G → S in allele ZF-3c. 1 Publication1
Natural varianti141T → A in allele ZF-3e. 1 Publication1
Natural varianti145K → R in allele ZF-3d and allele ZF-3e. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF382669 mRNA. Translation: ABQ23783.1.
BX465197 Genomic DNA. No translation available.
RefSeqiNP_001092923.1. NM_001099453.1.
UniGeneiDr.134029.

Genome annotation databases

GeneIDi798787.
KEGGidre:798787.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF382669 mRNA. Translation: ABQ23783.1.
BX465197 Genomic DNA. No translation available.
RefSeqiNP_001092923.1. NM_001099453.1.
UniGeneiDr.134029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VQ9X-ray1.85A23-149[»]
SMRiA5HAK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000123763.

Proteomic databases

PaxDbiA5HAK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi798787.
KEGGidre:798787.

Organism-specific databases

CTDi798787.
ZFINiZDB-GENE-050809-5. rnasel3.

Phylogenomic databases

eggNOGiENOG410IZBW. Eukaryota.
ENOG410Y4FD. LUCA.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiA5HAK0.
KOiK16631.
PhylomeDBiA5HAK0.

Enzyme and pathway databases

BRENDAi3.1.27.5. 928.

Miscellaneous databases

EvolutionaryTraceiA5HAK0.
PROiA5HAK0.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNSL3_DANRE
AccessioniPrimary (citable) accession number: A5HAK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: November 30, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Although the OmpT protease is absent in Gram-positive bacteria, LF-ZF3 generated by a Gram-negative bacterium can penetrate a Gram-positive bacterium and exert its cytotoxicity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.