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A5H0J2

- DUR1_LACKL

UniProt

A5H0J2 - DUR1_LACKL

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Protein

Urea amidolyase

Gene

DUR1,2

Organism
Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Involved in uracil catabolism. Hydrolysis of urea to ammonia and CO2.1 Publication

Catalytic activityi

ATP + urea + HCO3- = ADP + phosphate + urea-1-carboxylate.
Urea-1-carboxylate + H2O = 2 CO2 + 2 NH3.

Cofactori

Biotin.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei740 – 7401ATPBy similarity
Binding sitei823 – 8231ATPBy similarity
Binding sitei858 – 8581ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi115 – 1228ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. allophanate hydrolase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. biotin carboxylase activity Source: InterPro
  4. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro
  5. metal ion binding Source: InterPro
  6. urea carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. arginine metabolic process Source: UniProtKB-KW
  2. urea catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

ATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00258; UER00371.
UPA00258; UER00372.

Names & Taxonomyi

Protein namesi
Recommended name:
Urea amidolyase
Alternative name(s):
Pyrimidine-degrading protein 13,15
Uracil catabolism protein 3,5
Including the following 2 domains:
Urea carboxylase (EC:6.3.4.6)
Allophanate hydrolase (EC:3.5.1.54)
Gene namesi
Name:DUR1,2
Synonyms:PYD13,15, URC3,5
OrganismiLachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Taxonomic identifieri4934 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18301830Urea amidolyasePRO_0000367271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1796 – 17961N6-biotinyllysineBy similarity

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA5H0J2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 1068444Biotin carboxylationAdd
BLAST
Domaini744 – 941198ATP-graspPROSITE-ProRule annotationAdd
BLAST
Domaini1753 – 182977Biotinyl-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the DUR1,2 family.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
Contains 1 biotin carboxylation domain.Curated
Contains 1 biotinyl-binding domain.Curated

Family and domain databases

Gene3Di2.40.100.10. 2 hits.
3.30.1360.40. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.1300.10. 1 hit.
InterProiIPR003833. Allophan_hydro_1.
IPR003778. Allophan_hydro_2.
IPR014085. Allophanate_hydrolase.
IPR000120. Amidase.
IPR023631. Amidase_dom.
IPR024946. Arg_repress_C-like.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029000. Cyclophilin-like_dom.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
IPR014084. Urea_COase.
[Graphical view]
PfamiPF02682. AHS1. 1 hit.
PF02626. AHS2. 1 hit.
PF01425. Amidase. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00796. AHS1. 1 hit.
SM00797. AHS2. 1 hit.
SM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF50891. SSF50891. 2 hits.
SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR02713. allophanate_hyd. 1 hit.
TIGR00724. urea_amlyse_rel. 1 hit.
TIGR02712. urea_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5H0J2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVDTLGWSA QDWIDFHGKS TPEHSYNTLL SLLKSQKSAP EDPAWISLIN
60 70 80 90 100
EANLAHQWKV LQSKANKQQL PLYGVPIAVK DNIDSKGSPT TAACPAFEYN
110 120 130 140 150
PSADSTVVAL LKDAGAIVIG KTNLDQFATG LVGTRSPYGK TPCVFSDKHV
160 170 180 190 200
SGGSSAGSAS AVGRGIVPIA LGTDTAGSGR VPAALNNLIG LKPTKGLFSC
210 220 230 240 250
SGVVPACKSL DCVSVFAMNL SDAERCFKVM AKPDLENDEY SRPLPSNPLQ
260 270 280 290 300
KYPKNVTIAI PKEVPWYGET ENPKLYAKAI ENLKVAGASI VTIDFEPLLA
310 320 330 340 350
LARCLYEGAW VAERYEATKD FFATNPPESS LDPTVTSIIK TATKYDAADS
360 370 380 390 400
FRYEYQRQGI LQKVDQTLKD IDVLCVPTCP LNPTFEEVAA EPVLVNSRQG
410 420 430 440 450
TWTNFVNLAD MAALAVPAGF RPDGLPQGVT LIGKKFTDFA LLELANRYFK
460 470 480 490 500
VAFPQGSRTF GKFIDRQVTT KDDELRGPDI SPEDSVKLAV VGAHLKGLPL
510 520 530 540 550
YWQLEKVNAT YLGSPKTSKN YKLYALPKTG PILKPGLRRV GEETGSQIQL
560 570 580 590 600
EVYSVPKENF GEFISMVPEP LGIGSVELES GEWVKSFICE EFGYTQKGTV
610 620 630 640 650
DITKYGGFKK YIDFLKQEEA KVKKPFETVL IANRGEIAVR IIKTLKKLNI
660 670 680 690 700
RSVAVYSDPD KYSQHVIDAD LGVALNGRTA AETYLDIDKI IKAAKDTNAQ
710 720 730 740 750
AIIPGYGFLS ENAEFADKCV EEGIVFVGPS GEAIRKLGLK HSAREIAEKA
760 770 780 790 800
GVPLVPGSGL VTSAKEAKEI ANKLEYPVMV KSTAGGGGIG LQKVDSENEI
810 820 830 840 850
ERVFETVQHQ GKAYFGDSGV FLERFVENAR HVEIQMMGDG YGKAIAIGER
860 870 880 890 900
DCSLQRRNQK IIEETPAPNL GETTRTKMRQ AAESLGSLLK YKCAGTVEFI
910 920 930 940 950
YDERRDEFYF LEVNARLQVE HPITEMVTGL DLVEWMLRIA ADDAPDFESA
960 970 980 990 1000
NIVVTGASIE ARLYAENPAK DFRPSPGLLT DVHFPEWARV DTWVSKGTTV
1010 1020 1030 1040 1050
SAEYDPTLAK IIVHGKDRND AIMKMNKALN ETVVYGCITN IDYLRSIASS
1060 1070 1080 1090 1100
EMFKTAKVAT KILDSYDYKP CAFEVTSPGA YTTVQDYPGR VGYWRIGVPP
1110 1120 1130 1140 1150
SGPMDAYSFR LANRIVGNHY KAPAIELTLN GPKILFHTET IIAISGGIAA
1160 1170 1180 1190 1200
CSLNDKPIEQ NKPIQVNRGD HLAIGKLSVG CRAYLAIRGG IDVPEYLGSR
1210 1220 1230 1240 1250
STFALGNMGG YNGRVLKLGD VLFLNQPELA SSSLPGPAYE PQAPPANLLP
1260 1270 1280 1290 1300
KISDDKEWTI GVTCGPHGSP DFFKPESVEE FFSEKWKVHY NSNRFGVRLI
1310 1320 1330 1340 1350
GPKPKWARKD GGEGGLHPSN AHDYVYSLGA INFTGDEPVI ITSDGPSLGG
1360 1370 1380 1390 1400
FVCQAVVPEA ELWKVGQVKP GDSIQFVPIS YQVARQLKES QDAAIETLED
1410 1420 1430 1440 1450
GKLQTLTSDL ILPTYEDPVL VQLPKKSNLS PKVTYRQAGD RYILVEYGEN
1460 1470 1480 1490 1500
QMDLNIAYRI NQLINLVGKH KTVGIVEMSQ GVRSVLIEYD GYKISQGALL
1510 1520 1530 1540 1550
DTLVAYESEI QFDKNWSIKS KIFKLPLAFE DSKTLECVTR YQETIRSKAP
1560 1570 1580 1590 1600
WLPNNVDFVA EVNDITHKDV ENMLYSARFL VLGLGDVFLG APCAVPLDPR
1610 1620 1630 1640 1650
HRFLGSKYNP SRTYTKNGVV GIGGMYMCIY AMDSPGGYQL VGRTIPIWDK
1660 1670 1680 1690 1700
LKLGSHSQEH PWLLTPFDQV EFYPVSEEEL DRFTEDCENG KFPVQVEESV
1710 1720 1730 1740 1750
FDHKNYLKWI NENIESITEF QKSQGGAKAD EFARLIQVAN QELESSTTNK
1760 1770 1780 1790 1800
SAVEEEYPED AEMVYSEYSG RFWKPMVSAG DTVTKGDGLV IVEAMKTEMV
1810 1820 1830
VPAKKSGKVL KIVHKNGDMV DAGDLVAVIQ
Length:1,830
Mass (Da):201,131
Last modified:June 12, 2007 - v1
Checksum:iC5D1ED66A5F4F10D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ512718 Genomic DNA. Translation: ABF58890.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ512718 Genomic DNA. Translation: ABF58890.1 .

3D structure databases

ProteinModelPortali A5H0J2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00258 ; UER00371 .
UPA00258 ; UER00372 .

Family and domain databases

Gene3Di 2.40.100.10. 2 hits.
3.30.1360.40. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.1300.10. 1 hit.
InterProi IPR003833. Allophan_hydro_1.
IPR003778. Allophan_hydro_2.
IPR014085. Allophanate_hydrolase.
IPR000120. Amidase.
IPR023631. Amidase_dom.
IPR024946. Arg_repress_C-like.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029000. Cyclophilin-like_dom.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
IPR014084. Urea_COase.
[Graphical view ]
Pfami PF02682. AHS1. 1 hit.
PF02626. AHS2. 1 hit.
PF01425. Amidase. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view ]
SMARTi SM00796. AHS1. 1 hit.
SM00797. AHS2. 1 hit.
SM00878. Biotin_carb_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50891. SSF50891. 2 hits.
SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF75304. SSF75304. 1 hit.
TIGRFAMsi TIGR02713. allophanate_hyd. 1 hit.
TIGR00724. urea_amlyse_rel. 1 hit.
TIGR02712. urea_carbox. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Entry informationi

Entry nameiDUR1_LACKL
AccessioniPrimary (citable) accession number: A5H0J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 12, 2007
Last modified: October 1, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3