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A5H0J2

- DUR1_LACKL

UniProt

A5H0J2 - DUR1_LACKL

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Protein

Urea amidolyase

Gene
DUR1,2, PYD13,15, URC3,5
Organism
Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in uracil catabolism. Hydrolysis of urea to ammonia and CO2.1 Publication

Catalytic activityi

ATP + urea + HCO3- = ADP + phosphate + urea-1-carboxylate.
Urea-1-carboxylate + H2O = 2 CO2 + 2 NH3.

Cofactori

Biotin By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei740 – 7401ATP By similarity
Binding sitei823 – 8231ATP By similarity
Binding sitei858 – 8581ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi115 – 1228ATP Reviewed prediction

GO - Molecular functioni

  1. allophanate hydrolase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. biotin carboxylase activity Source: InterPro
  4. carbon-nitrogen ligase activity, with glutamine as amido-N-donor Source: InterPro
  5. metal ion binding Source: InterPro
  6. urea carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. arginine metabolic process Source: UniProtKB-KW
  2. urea catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

ATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00258; UER00371.
UPA00258; UER00372.

Names & Taxonomyi

Protein namesi
Recommended name:
Urea amidolyase
Alternative name(s):
Pyrimidine-degrading protein 13,15
Uracil catabolism protein 3,5
Including the following 2 domains:
Urea carboxylase (EC:6.3.4.6)
Allophanate hydrolase (EC:3.5.1.54)
Gene namesi
Name:DUR1,2
Synonyms:PYD13,15, URC3,5
OrganismiLachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Taxonomic identifieri4934 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18301830Urea amidolyasePRO_0000367271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1796 – 17961N6-biotinyllysine By similarity

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliA5H0J2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 1068444Biotin carboxylationAdd
BLAST
Domaini744 – 941198ATP-graspAdd
BLAST
Domaini1753 – 182977Biotinyl-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the DUR1,2 family.
Contains 1 ATP-grasp domain.

Family and domain databases

Gene3Di2.40.100.10. 2 hits.
3.30.1360.40. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.1300.10. 1 hit.
InterProiIPR003833. Allophan_hydro_1.
IPR003778. Allophan_hydro_2.
IPR014085. Allophanate_hydrolase.
IPR000120. Amidase.
IPR023631. Amidase_dom.
IPR024946. Arg_repress_C-like.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029000. Cyclophilin-like_dom.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
IPR014084. Urea_COase.
[Graphical view]
PfamiPF02682. AHS1. 1 hit.
PF02626. AHS2. 1 hit.
PF01425. Amidase. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00796. AHS1. 1 hit.
SM00797. AHS2. 1 hit.
SM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF50891. SSF50891. 2 hits.
SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR02713. allophanate_hyd. 1 hit.
TIGR00724. urea_amlyse_rel. 1 hit.
TIGR02712. urea_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5H0J2-1 [UniParc]FASTAAdd to Basket

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MSVDTLGWSA QDWIDFHGKS TPEHSYNTLL SLLKSQKSAP EDPAWISLIN     50
EANLAHQWKV LQSKANKQQL PLYGVPIAVK DNIDSKGSPT TAACPAFEYN 100
PSADSTVVAL LKDAGAIVIG KTNLDQFATG LVGTRSPYGK TPCVFSDKHV 150
SGGSSAGSAS AVGRGIVPIA LGTDTAGSGR VPAALNNLIG LKPTKGLFSC 200
SGVVPACKSL DCVSVFAMNL SDAERCFKVM AKPDLENDEY SRPLPSNPLQ 250
KYPKNVTIAI PKEVPWYGET ENPKLYAKAI ENLKVAGASI VTIDFEPLLA 300
LARCLYEGAW VAERYEATKD FFATNPPESS LDPTVTSIIK TATKYDAADS 350
FRYEYQRQGI LQKVDQTLKD IDVLCVPTCP LNPTFEEVAA EPVLVNSRQG 400
TWTNFVNLAD MAALAVPAGF RPDGLPQGVT LIGKKFTDFA LLELANRYFK 450
VAFPQGSRTF GKFIDRQVTT KDDELRGPDI SPEDSVKLAV VGAHLKGLPL 500
YWQLEKVNAT YLGSPKTSKN YKLYALPKTG PILKPGLRRV GEETGSQIQL 550
EVYSVPKENF GEFISMVPEP LGIGSVELES GEWVKSFICE EFGYTQKGTV 600
DITKYGGFKK YIDFLKQEEA KVKKPFETVL IANRGEIAVR IIKTLKKLNI 650
RSVAVYSDPD KYSQHVIDAD LGVALNGRTA AETYLDIDKI IKAAKDTNAQ 700
AIIPGYGFLS ENAEFADKCV EEGIVFVGPS GEAIRKLGLK HSAREIAEKA 750
GVPLVPGSGL VTSAKEAKEI ANKLEYPVMV KSTAGGGGIG LQKVDSENEI 800
ERVFETVQHQ GKAYFGDSGV FLERFVENAR HVEIQMMGDG YGKAIAIGER 850
DCSLQRRNQK IIEETPAPNL GETTRTKMRQ AAESLGSLLK YKCAGTVEFI 900
YDERRDEFYF LEVNARLQVE HPITEMVTGL DLVEWMLRIA ADDAPDFESA 950
NIVVTGASIE ARLYAENPAK DFRPSPGLLT DVHFPEWARV DTWVSKGTTV 1000
SAEYDPTLAK IIVHGKDRND AIMKMNKALN ETVVYGCITN IDYLRSIASS 1050
EMFKTAKVAT KILDSYDYKP CAFEVTSPGA YTTVQDYPGR VGYWRIGVPP 1100
SGPMDAYSFR LANRIVGNHY KAPAIELTLN GPKILFHTET IIAISGGIAA 1150
CSLNDKPIEQ NKPIQVNRGD HLAIGKLSVG CRAYLAIRGG IDVPEYLGSR 1200
STFALGNMGG YNGRVLKLGD VLFLNQPELA SSSLPGPAYE PQAPPANLLP 1250
KISDDKEWTI GVTCGPHGSP DFFKPESVEE FFSEKWKVHY NSNRFGVRLI 1300
GPKPKWARKD GGEGGLHPSN AHDYVYSLGA INFTGDEPVI ITSDGPSLGG 1350
FVCQAVVPEA ELWKVGQVKP GDSIQFVPIS YQVARQLKES QDAAIETLED 1400
GKLQTLTSDL ILPTYEDPVL VQLPKKSNLS PKVTYRQAGD RYILVEYGEN 1450
QMDLNIAYRI NQLINLVGKH KTVGIVEMSQ GVRSVLIEYD GYKISQGALL 1500
DTLVAYESEI QFDKNWSIKS KIFKLPLAFE DSKTLECVTR YQETIRSKAP 1550
WLPNNVDFVA EVNDITHKDV ENMLYSARFL VLGLGDVFLG APCAVPLDPR 1600
HRFLGSKYNP SRTYTKNGVV GIGGMYMCIY AMDSPGGYQL VGRTIPIWDK 1650
LKLGSHSQEH PWLLTPFDQV EFYPVSEEEL DRFTEDCENG KFPVQVEESV 1700
FDHKNYLKWI NENIESITEF QKSQGGAKAD EFARLIQVAN QELESSTTNK 1750
SAVEEEYPED AEMVYSEYSG RFWKPMVSAG DTVTKGDGLV IVEAMKTEMV 1800
VPAKKSGKVL KIVHKNGDMV DAGDLVAVIQ 1830
Length:1,830
Mass (Da):201,131
Last modified:June 12, 2007 - v1
Checksum:iC5D1ED66A5F4F10D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ512718 Genomic DNA. Translation: ABF58890.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ512718 Genomic DNA. Translation: ABF58890.1 .

3D structure databases

ProteinModelPortali A5H0J2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00258 ; UER00371 .
UPA00258 ; UER00372 .

Family and domain databases

Gene3Di 2.40.100.10. 2 hits.
3.30.1360.40. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.1300.10. 1 hit.
InterProi IPR003833. Allophan_hydro_1.
IPR003778. Allophan_hydro_2.
IPR014085. Allophanate_hydrolase.
IPR000120. Amidase.
IPR023631. Amidase_dom.
IPR024946. Arg_repress_C-like.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029000. Cyclophilin-like_dom.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
IPR014084. Urea_COase.
[Graphical view ]
Pfami PF02682. AHS1. 1 hit.
PF02626. AHS2. 1 hit.
PF01425. Amidase. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view ]
SMARTi SM00796. AHS1. 1 hit.
SM00797. AHS2. 1 hit.
SM00878. Biotin_carb_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50891. SSF50891. 2 hits.
SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF75304. SSF75304. 1 hit.
TIGRFAMsi TIGR02713. allophanate_hyd. 1 hit.
TIGR00724. urea_amlyse_rel. 1 hit.
TIGR02712. urea_carbox. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Entry informationi

Entry nameiDUR1_LACKL
AccessioniPrimary (citable) accession number: A5H0J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi