A5GVR3 (PANCY_SYNR3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional pantoate ligase/cytidylate kinase

Including the following 2 domains:

1. Pantothenate synthetase
   Short name=PS
   EC=6.3.2.1
   Alternative name(s):
   Pantoate--beta-alanine ligase
   Pantoate-activating enzyme
2. Cytidylate kinase
   Short name=CK
   EC=2.7.4.14
   Alternative name(s):
   Cytidine monophosphate kinase
   Short name=CMP kinase

Gene names

Name:	panC/cmk
Ordered Locus Names:	SynRCC307_2069

Organism

Synechococcus sp. (strain RCC307) [Complete proteome] [HAMAP]

Taxonomic identifier

316278 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP-Rule MF_01349 Displays a CMP kinase activity By similarity.
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP-Rule MF_01349 ATP + (d)CMP = ADP + (d)CDP. HAMAP-Rule MF_01349
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP-Rule MF_01349
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the pantothenate synthetase family. In the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cytidylate kinase activity Inferred from electronic annotation. Source: EC pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Bifunctional pantoate ligase/cytidylate kinase HAMAP-Rule MF_01349		PRO_0000333301
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity
	Nucleotide binding	147 – 150	4	ATP By similarity
	Nucleotide binding	184 – 187	4	ATP By similarity
	Region	1 – 276	276	Pantoate--beta-alanine ligase HAMAP-Rule MF_01349
	Region	277 – 512	236	Cytidylate kinase HAMAP-Rule MF_01349
Sites
	Active site	34	1	Proton donor By similarity
	Binding site	58	1	Beta-alanine By similarity
	Binding site	58	1	Pantoate By similarity
	Binding site	153	1	Pantoate By similarity
	Binding site	176	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5GVR3 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: B6ACF2E754363B2E	FASTA	512	55,206
	10 20 30 40 50 60 MKLQTSADLQ RWLQAGGPGP LHLVPTMGAL HQGHAALIRA ARQQGGRVLV SVFVNPLQFS 70 80 90 100 110 120 PNEDFARYPR RLEEDHALAL EAGADALWAP QPEDVFPAGA AGLTQLAPAP ELVANLCGPS 130 140 150 160 170 180 RPGHFEGVCT VVSRLLALVQ PSHLHLGEKD WQQLQVLRRL VRDLRWPVQI VPCPTLRERD 190 200 210 220 230 240 GLPLSSRNAY LSVEQRQQAA LLPQALAQGQ QLLDAGQRQA EPLLRAVRAL MEDGGLAVDY 250 260 270 280 290 300 LQLVDLPRLQ ELEQVTGPAL LAAAVRCGEA RLIDHRVLMS RLPILAIDGP AGAGKSTVTR 310 320 330 340 350 360 QVAHELGLTY LDTGAMYRGV TWLLQQRGFE PQEGEPLQAL LADLELRFGP ASGTEQTLLV 370 380 390 400 410 420 NGVDATSAIR TAEVTASVSA VAALPSVRAA LTQQQQQLGQ QGGLVAEGRD IGTAVFPDAE 430 440 450 460 470 480 LKIYLTATVA ERARRRAADL AARSLPVPVL SQLEQEIADR DHKDSSREVA PLRQASDAVE 490 500 510 LLSDGLSIDE VVAQIVALFR EWVPVEALNA DA

References

[1]	Genoscope Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RCC307.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT978603 Genomic DNA. Translation: CAK28972.1.
RefSeq	YP_001228325.1. NC_009482.1.
3D structure databases
ProteinModelPortal	A5GVR3.
ModBase	Search...
Protein-protein interaction databases
STRING	316278.SynRCC307_2069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAK28972; CAK28972; SynRCC307_2069.
GeneID	5156505.
KEGG	syr:SynRCC307_2069.
PATRIC	23825069. VBISynSp108374_2078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0283.
HOGENOM	HOG000233355.
KO	K13799.
OMA	LGEKDWQ.
ProtClustDB	PRK13477.
Enzyme and pathway databases
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01349. PanCY.
InterPro	IPR004821. Cyt_trans-like. IPR003136. Cytidylate_kin. IPR011994. Cytidylate_kinase_dom. IPR003721. Pantoate_ligase. IPR024894. Pantoate_ligase/cytidylate_kin. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02224. Cytidylate_kin. 1 hit. PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00017. cmk. 1 hit. TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANCY_SYNR3

Accession

Primary (citable) accession number: A5GVR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program