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A5GS37

- BIOB_SYNR3

UniProt

A5GS37 - BIOB_SYNR3

Protein

Biotin synthase

Gene

bioB

Organism
Synechococcus sp. (strain RCC307)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:SynRCC307_0793
    OrganismiSynechococcus sp. (strain RCC307)
    Taxonomic identifieri316278 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001115: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Biotin synthasePRO_0000381679Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi316278.SynRCC307_0793.

    Structurei

    3D structure databases

    ProteinModelPortaliA5GS37.
    SMRiA5GS37. Positions 6-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5GS37-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALIRHDWD LAEIEALLEL PLVDLLWHAQ QVHRDAHPAG YHVQLASLLS    50
    VKTGGCEEDC AYCPQSMHHS SDVTAQPELQ LQVDGVLQQA RSARDAGAHR 100
    FCMGWAWREI REGAPFDAML AMVRGVRELG MEACVTAGML TDSQAERLAQ 150
    AGLTSYNHNL DTSPEHYDQI ISTRTYQERL DTLQRARDAG LSLCCGGIIG 200
    MGESRRDRAS LLQVLAGLNP HPESVPINGL VAVEGTPLED QEPWEPLELV 250
    RMVAVARILM PEARVRLSAG RERLSQEAQI LCLLAGADSI FYGDSLLTTS 300
    NPAVQADQAL LAAAGVSSSL EGVAAA 326
    Length:326
    Mass (Da):35,348
    Last modified:June 12, 2007 - v1
    Checksum:i7E72DDC9ECB4464A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT978603 Genomic DNA. Translation: CAK27696.1.
    RefSeqiWP_011935210.1. NC_009482.1.
    YP_001227049.1. NC_009482.1.

    Genome annotation databases

    EnsemblBacteriaiCAK27696; CAK27696; SynRCC307_0793.
    GeneIDi5156357.
    KEGGisyr:SynRCC307_0793.
    PATRICi23822456. VBISynSp108374_0785.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT978603 Genomic DNA. Translation: CAK27696.1 .
    RefSeqi WP_011935210.1. NC_009482.1.
    YP_001227049.1. NC_009482.1.

    3D structure databases

    ProteinModelPortali A5GS37.
    SMRi A5GS37. Positions 6-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316278.SynRCC307_0793.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK27696 ; CAK27696 ; SynRCC307_0793 .
    GeneIDi 5156357.
    KEGGi syr:SynRCC307_0793.
    PATRICi 23822456. VBISynSp108374_0785.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Genoscope
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RCC307.

    Entry informationi

    Entry nameiBIOB_SYNR3
    AccessioniPrimary (citable) accession number: A5GS37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3