Reviewed, UniProtKB/Swiss-Prot A5GR42 (MTNC_SYNR3)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	SynRCC307_0448

Organism

Synechococcus sp. (strain RCC307) [Complete proteome] [HAMAP]

Taxonomic identifier

316278 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP methionine salvage Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: HAMAP 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: HAMAP acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357423

Sequences

	Sequence		Length	Mass (Da)
	A5GR42-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: DBA4F9D2FEE0CD60	FASTA	249	27,233
	10 20 30 40 50 60 MIDWESLRAI VLDIEGTTCP VDFVTGSLFP YARQHLGTLL SQDDQQAPLK PLLDEVRIAW 70 80 90 100 110 120 KHENSAEAPA YSDSQDPLAL LPYLQWLIDQ DRKLAPLKEL QGLTWRHGYQ SGALTTPLFA 130 140 150 160 170 180 DVAPALKRWQ QRGLRLAVYS SGSVAAQQLF YGHTSDGDLS DLFERWYDTR LGPKNEAQSY 190 200 210 220 230 240 TLLAADLQLP AHAVLFISDS SGELAAAQAA GLQICGSQRP GNPETLSAKW PVVVSSLEAL APSGPPGLR

References

[1]	Genoscope Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CT978603 Genomic DNA. Translation: CAK27351.1.
RefSeq	YP_001226704.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5GR42.
Genome annotation databases
GeneID	5156583.
GenomeReviews	Gene locus SynRCC307_0448 in contig CT978603_GR.
KEGG	syr:SynRCC307_0448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TTDLNFI.
Family and domain databases
HAMAP	MF_01681. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_SYNR3

Accession

Primary (citable) accession number: A5GR42

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)