A5GMU7 (SYE_SYNPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	SynWH7803_1836

Organism

Synechococcus sp. (strain WH7803) [Complete proteome] [HAMAP]

Taxonomic identifier

32051 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001979
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	247 – 251	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	250	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5GMU7 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 1DB73B3D313451B8	FASTA	476	53,105
	10 20 30 40 50 60 MVRVRLAPSP TGTLHIGTAR TAVFNWLFAR NQNGSFLLRI EDTDKERSKP EFTQNILEGL 70 80 90 100 110 120 QWLGLDWDGD PVIQSEQTER HRSAIQTLLE KGLAYRCYAS EEELDAMRTQ QKAANQAPRY 130 140 150 160 170 180 DNRHRDLTPE QEAAFQAEGR DAVIRFRIDE HREIRWNDLV RGPMLWRGAD LGGDMVVARR 190 200 210 220 230 240 APADQIGDPL YNLVVVIDDA AMAISHVIRG EDHIANTAKQ LLLYEALSLP VPTFAHAPLI 250 260 270 280 290 300 LNPEGRKLSK RDGVTSINDF RAMGYTPEAL ANYMTLLGWS VPEGMNEIFT LEAAAKVFDF 310 320 330 340 350 360 DRVNKAGAKF DWDKLNWLNA QVLHSWEPAN LLNALAPLWA DQGWTLPDTT GWSLSLVELL 370 380 390 400 410 420 GPSLTLLNDG VDQAKPFFME PALEDDALKQ LDQEGAKACL QALLSTLEEA PWSGESIDQA 430 440 450 460 470 QEMLKSAAAT AGVKKGILMK SLRAALLGRL QGPDLLTTWS LLAQIGQDLP RLRRCL

References

[1]	Genoscope Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH7803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT971583 Genomic DNA. Translation: CAK24262.1.
RefSeq	YP_001225559.1. NC_009481.1.
3D structure databases
ProteinModelPortal	A5GMU7.
SMR	A5GMU7. Positions 1-476.
ModBase	Search...
Protein-protein interaction databases
STRING	A5GMU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5146563.
GenomeReviews	Gene locus SynWH7803_1836 in contig CT971583_GR.
KEGG	syx:SynWH7803_1836.
PATRIC	23829867. VBISynSp43824_1882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	WENVRVR.
PhylomeDB	A5GMU7.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	SSP32051:SYNWH7803_1836-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_SYNPW

Accession

Primary (citable) accession number: A5GMU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program