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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Synechococcus sp. (strain WH7803)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi139 – 1391MagnesiumUniRule annotation
Metal bindingi142 – 1421MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:SynWH7803_1601
OrganismiSynechococcus sp. (strain WH7803)
Taxonomic identifieri32051 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001566: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Crossover junction endodeoxyribonuclease RuvCPRO_1000002842Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi32051.SynWH7803_1601.

Structurei

3D structure databases

ProteinModelPortaliA5GM62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiMQIKLAL.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5GM62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILGIDPGL ARVGYGVIET AGREQTMLDC GIIRTDSGRP EGERMVEIAR
60 70 80 90 100
DLRQLIRAWK PELAAVEKFF FYRSSNTIAV VQARGVVIMT LSRFGLPIVE
110 120 130 140 150
FPPMQIKQAL TGHGHADKDE VLEAVMRELS LETPPRPDDA ADALAVALTG

WFQR
Length:154
Mass (Da):17,104
Last modified:June 12, 2007 - v1
Checksum:i38DAF6E2236504E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT971583 Genomic DNA. Translation: CAK24027.1.
RefSeqiWP_011933502.1. NC_009481.1.
YP_001225324.1. NC_009481.1.

Genome annotation databases

EnsemblBacteriaiCAK24027; CAK24027; SynWH7803_1601.
GeneIDi5147638.
KEGGisyx:SynWH7803_1601.
PATRICi23829361. VBISynSp43824_1636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT971583 Genomic DNA. Translation: CAK24027.1.
RefSeqiWP_011933502.1. NC_009481.1.
YP_001225324.1. NC_009481.1.

3D structure databases

ProteinModelPortaliA5GM62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi32051.SynWH7803_1601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK24027; CAK24027; SynWH7803_1601.
GeneIDi5147638.
KEGGisyx:SynWH7803_1601.
PATRICi23829361. VBISynSp43824_1636.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiMQIKLAL.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Genoscope
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WH7803.

Entry informationi

Entry nameiRUVC_SYNPW
AccessioniPrimary (citable) accession number: A5GM62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.