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A5GLZ1

- BIOB_SYNPW

UniProt

A5GLZ1 - BIOB_SYNPW

Protein

Biotin synthase

Gene

bioB

Organism
Synechococcus sp. (strain WH7803)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi101 – 1011Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi193 – 1931Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi265 – 2651Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:SynWH7803_1530
    OrganismiSynechococcus sp. (strain WH7803)
    Taxonomic identifieri32051 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001566: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Biotin synthasePRO_0000381677Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi32051.SynWH7803_1530.

    Structurei

    3D structure databases

    ProteinModelPortaliA5GLZ1.
    SMRiA5GLZ1. Positions 8-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiIDLMWEA.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5GLZ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEQVDLRHD WTLAEIEALL QSPLMDLLWK AQAVHRSANP GYKVQLASLL    50
    SVKTGGCEED CAYCPQSMHH SSDVTGQPDL EVKAVLDRAR VAAEAGADRF 100
    CMGWAWREIR EGPAFESMLS MVRGVRELGL EACVTAGMLT DSQAERLAEA 150
    GLTAYNHNLD TSPEHYDSII TTRTYQERLE TLQRVRQAGV TLCCGGIIGM 200
    GESVRDRASM LQVLACLDPH PESVPINALV AVEGTPLEAQ PSIDPLELVR 250
    MVAVARILMP QSRVRLSAGR EQLNREAQIL CLQAGADSIF YGDSLLTTSN 300
    PAVESDRALL AAAGVQASWH ESAAA 325
    Length:325
    Mass (Da):35,280
    Last modified:June 12, 2007 - v1
    Checksum:i7783BAA4BD766691
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT971583 Genomic DNA. Translation: CAK23956.1.
    RefSeqiWP_011933433.1. NC_009481.1.
    YP_001225253.1. NC_009481.1.

    Genome annotation databases

    EnsemblBacteriaiCAK23956; CAK23956; SynWH7803_1530.
    GeneIDi5148297.
    KEGGisyx:SynWH7803_1530.
    PATRICi23829212. VBISynSp43824_1563.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT971583 Genomic DNA. Translation: CAK23956.1 .
    RefSeqi WP_011933433.1. NC_009481.1.
    YP_001225253.1. NC_009481.1.

    3D structure databases

    ProteinModelPortali A5GLZ1.
    SMRi A5GLZ1. Positions 8-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 32051.SynWH7803_1530.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK23956 ; CAK23956 ; SynWH7803_1530 .
    GeneIDi 5148297.
    KEGGi syx:SynWH7803_1530.
    PATRICi 23829212. VBISynSp43824_1563.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi IDLMWEA.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Genoscope
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WH7803.

    Entry informationi

    Entry nameiBIOB_SYNPW
    AccessioniPrimary (citable) accession number: A5GLZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3